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    Analysis of human omentum-associated lymphoid tissue components with S-100: an immunohistochemical study
    (Editura Acad Romane, 2010) Yildirim, A.; Aktas, A.; Nergiz, Y.; Akkus, M.
    Milky spots are opaque patches in the greater omentum. They were first described by von Recklinghausen (1863) in the omentum of rabbits. In man, milky spots are relatively uniform, highly vascularized accumulations of mononuclear cells. The objective of this study was to describe in human omental lymphoid tissue components with S-100. Tissue samples (greater omentum) were collected from 14 patients operated with different reasons in our Department of General Surgery, in order to histologically present the presence of S-100 in the cells making up the milky spots in human omentum tissue. Tissue samples were cut approximately 5-8 micrometer thick with frozen-sections and stained with an indirect immunoperoxidase technique, as described previously. Then milky spots were examined by light microscopy. These data indicate that unstimulated milky spots in the human greater omentum are to a great extent just a preformed specific accumulation of primarily macrophages within the stroma of the greater omentum, secondarily B-and T-lymphocytes. In addition to these cells, we observed that a few mast and reticular cells were seen in the milky spots by S-100 reactive cross-sections of greater omentum. In the human omentum tissue that was stained with indirect immunoperoxidase method using anti S-100 monoclonal antibody, an arteriole cross-section in the center, reactive nerve cross-sections in the adjacent stroma and endogenic peroxidase reactivity in a few granulocytes in omental tissue were observed.
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    EFFECTS OF ETHYL PYRUVATE ON TESTICULAR DAMAGE IN RATS WITH STREPTOZOTOCIN-INDUCED DIABETES
    (Editura Acad Romane, 2012) Akkoc, H.; Kelle, I.; Tunik, S.; Erdinc, M.; Erdinc, L.; Nergiz, Y.
    Diabetes mellitus is associated with impairment of testicular functions. Aim. The present study aimed to investigate the effects of ethyl pyruvate (EP) on the testicular tissue damage in rats with streptozotocin (STZ)-induced diabetes. Subjects and methods. Thirty-two Wistar albino male rats were assigned into four equal groups as follows: (1) control group (n:8); (2) EP-treated non-diabetic group (n:8); (3) diabetic group (n:8); and (4) EP-treated diabetic group (n:8). Rats with STZ-induced diabetes were kept alive for 14 weeks. After that, the EP solution was administered intraperitoneally to the rats in the EP-treated non-diabetic and diabetic groups at the dose of 50 mg/kg twice daily for 14 clays. At the end of this period, the left testes were removed from the rats for malondialdehyde (MDA) analysis, and the right testes were removed for histological examination. Results. As compared with the control group, the diabetic group had elevated MDA levels (210.9 +/- 12.7) and increased thickness of the basement membrane of the seminiferous tubules (3.01 +/- 0.16), but decreased tubular diameter (159 +/- 9.0) and Johnsen's score (5.31 +/- 0.1). In the EP-treated diabetic group, diabetes-induced impairment was significantly improved. Conclusion. These findings indicate that EP shows protective effects against diabetes-induced testicular dysfunction.
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    Effects of mesenchymal stem cells in critical size bone defect
    (Verduci Publisher, 2012) Agacayak, S.; Gulsun, B.; Ucan, M. C.; Karaoz, E.; Nergiz, Y.
    Background and Objectives: The aim of this study was to compare culture-expanded, bone marrow-derived mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) loaded to biphasic calcium phosphate (BCP) bone ceramic in the repair of rat calvarial bone. Materials and Methods: Critical-size (7 mm dia.) calvarial defects were prepared in the frontal-parietal bones of 90 adult female Sprague-Dawley rats. Rats were randomly divided into 5 groups, according to defect filling, as follows: Group I (n=21), BCP; Group II (n=21), BCP+PRP; Group III (n=21), BCP+MSC; Group IV (n=21), BCP+PRP+MSC; Group V (n=6) (control), no treatment. Animals were sacrificed at 2, 8 and 12 weeks postsurgery and bone regeneration was evaluated both histologically and immunohistochemically. Results: Statistically significant differences were observed in bone osteoblastic activity in calvarial defects among the groups (p < 0.05). PRP and MSC used in combination with BCP as a defect filling resulted in greater osteoblastic bone formation activity when compared to the use of BCP alone. Conclusions: The combination of mesenchymal stem cells, platelet rich plasma and synthetic bone substitute was found to be more effective in inducing new bone formation (osteogenesis) than the use of platelet rich plasma combined with synthetic bone substitute and the use of synthetic bone substitute alone.
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    Effects of N-acetyl-cysteine and acetylsalicylic acid on the tonsil bacterial biofilm tissues by light and electron microscopy
    (Verduci Publisher, 2014) Bulut, F.; Meric, F.; Yorgancilar, E.; Nergiz, Y.; Akkus, M.; Nergiz, S.; Nasir, Y.
    OBJECTIVE: The present study aimed to investigate the effects of the bacterial biofilm formation on the tonsil surface exposed N-acetyl-cysteine (NAC) and acetylsalicylic acid (ASA) of patients undergoing tonsillectomy by light and electron microscopy. The general process of biofilm formation comprises adhesion of free-living or planktonic bacteria to a surface, which subsequently develop into microcolonies and form a biofilm. Based on studies that have shown the presence of biofilms in common sites of chronic infections, it has become clear that bacteria may persist on mucosal surfaces through formation of biofilms. PATIENTS AND METHODS: Ten patients between 4 and 39 years of age (mean, 11.9 +/- 11.2 years). In all cases, periodic acide Schiff (PAS) staining was found to be an accurate predictor of the presence or absence of biofilm using light microscopy as a control standard. Therapeutic doses of NAC and ASA were identificated as the effective on the tonsil bacterial biofilm using light and electron microscopy. RESULTS: Biofilm formation was detected on all samples. Tonsils removed from patients with ASA-10 had showed higher-grade inhibitory effect at the biofilm formation than the other group (p <= 0.0001). The correlation was found between drug dose and decrease at the biofilm formation. CONCLUSIONS: In chronic or recurrent tonsillitis patients, decrease on the tonsils surface biofilm formation may be associated with ASA dose. Whether effect on the tonsils surface biofilm formation of other agent have a role is not known.
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    Investigation of the protective effects of melatonin, amifostine (WR-2721), and N-acetylcysteine on radiotherapy-induced uterine tissue injury in rats
    (Ijrr-Iranian Journal Radiation Res, 2020) Seker, U.; Aktas, A.; Nergiz, Y.; Zincircioglu, S. B.; Ketani, M. A.
    Background: The aim of this study was to investigate the protective potency of melatonin, amifostine (WR-2721), and N-acetylcysteine (NAC) when administered intraperitoneally (i.p.) 15 min before 10-Gy single-fraction radiotherapy. Materials and Methods: In this study, 35 female Sprague Dawley rats were divided into five groups of seven rats each. The rats in the control group did not receive any treatments. Rats in the radiotherapy, melatonin, amifostine, and NAC groups underwent abdomino-pelvic irradiation with 10-Gy single fraction gamma (gamma) irradiation. Melatonin 50 mg/kg, amifostine 200 mg/kg, and NAC 500 mg/kg were i.p. administered to the rats 15 min before irradiation. Animals were sacrificed 48 h after irradiation. Uterus samples were collected and, routine histopathological tissue processing was performed. Sections from tissue samples were stained with H&E and analyzed with the terminal deoxynucleotidyl transferase dUTP nick end labelling method (TUNEL assay). Results: Severe morphological degenerations and increases in the apoptotic index (AI) were observed in the radiotherapy group. Tissue protection and AI reduction were observed in the amifostine and NAC groups. Melatonin was more effective than amifostine and NAC. Morphological damage was almost completely repaired, and the AI of the melatonin group was quite similar to that of the control group. Conclusion: This experiment failed to determine a more successful administration technique of amifostine. The protective effects of amifostine and NAC were similar. Melatonin was more successful than these two drugs, and might be an alternative to amifostine when time, dose, or adverse effect constraints are encountered.
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    Protective effect of ethyl pyruvate on liver injury in streptozotocin-induced diabetic rats
    (Univ Catholique Louvain-Ucl, 2012) Akkoc, H.; Kelle, I.; Tunik, S.; Bahceci, S.; Sencar, L.; Ayaz, E.; Nergiz, Y.
    Background and aims : Diabetes Mellitus, leading to an increase in oxidative stress, can cause liver damage. Our aim was to investigate the antioxidant effects of Ethyl Pyruvate (EP) on the liver tissue in diabetic rats. Materials and methods : Thirty-two Wistar albino rats were separated into four equal groups. Groups were assigned as follows : (1) Non-diabetic group; (2) EP-treated non-diabetic group; (3) diabetic group; and (4) EP-treated diabetic group. In order to induce diabetes mellitus, 45 mg/kg b.w. streptozotocin was administered intraperitoneally to the rats in groups 3 and 4. On the 3rd day, blood glucose was assessed. Rats with blood glucose levels higher than 300 mg/dl were considered to be diabetic. The EP solution was administered intraperitoneally at a dose of 50 mg/kg b.w. twice daily for 14 days to the rats in groups 2 and 4. The other rats were simultaneously given the same amount of Ringer's lactate solution intraperitoneally. Liver tissue was obtained for malondialdehyde (MDA) analyses and histopathological examination. Results : In group 4, Total Antioxidant Status (TOS) and MDA levels were significantly lower as compared to group 3. Also, morphological abnormalities occurred in group 3 when compared with non-diabetic groups (groups 1 and 2), whereas the disorders resulting from diabetes improved significantly in group 4. Conclusions : These findings show that EP has protective effects against diabetes-induced liver injury. (Acta gastroenterol. belg, 2012. 75, 336-341).