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    Öğe
    A key gene bHLH115 in iron homeostasis: comprehensive bioinformatics analyses in Arabidopsis, tomato, rice, and maize
    (Springer, 2019) Kurt, Firat; Filiz, Ertugrul; Kurt, Baris
    bHLH115 transcription factor (TF) is a positive regulator of the Fe-deficiency and plays essential roles in the stress-related regulation network. In this study, orthologous bHLH115 genes in Arabidopsis, tomato, rice, and maize were analyzed using in silico methods. All bHLH115 proteins contained PF00010 (HLH: Helix-loop-helix DNA-binding domain) domain structure andtheir sub-cellular localizations were predicted as nucleus. The bHLH115 orthologues in monocots and dicots clearly diverged from each other. The expression analyses revealed that orthologous genes of bHLH115 in queried species were highly expressed in seed parts, leaf, stem, and flower parts. The bHLH115 genes were co-expressed with genes in plant defense system, and withgenes involving inbiotic and abiotic stress responses. In terms of protein structures, OsbHLH115 and ZmbHLH115, and AtbHLH115 and SlbHLH115 had the highest protein structure similarities. In addition, bHLH115 proteins have bZIP, bHLH and MYB transcription factor binding sites strengthens their engagement in various metabolic ways. Molecular docking analyses showed the different binding sites based on plant species, suggesting functional flexibilities of bHLH115 gene.
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    Öğe
    Synthesis, characterization, DNA interaction and docking studies of novel Schiff base ligand derived from 2,6-diaminopyridine and its complexes
    (Elsevier, 2020) Kurt, Baris; Temel, Hamdi; Atlan, Metin; Kaya, Savas
    In this study [1-(4-{[(3-hydroxynaphthalen-2-yl)methylidene]amino}phenyl)ethan-1- one (B-1 ligand) were synthesized from the reaction of 2-hydroxy-1-naphthaldehyde with 4-amino-acetophenone. Following that 1-(N-{4-[(1)-1-({6-[(Z)-[1-(4-{[(3- hydroxynaphthalen-2-yl) methylidene]amino}phenyl) ethylidene]amino]pyridine2- yl}imino)ethyl]phenyl}carboximidoyl)naphthalen-2-ol (L-1 ligand) were synthesized from the reaction of 2,6-diamino pyridine with B-1 ligand. Using this Schiff base ligand Cu(II), Fe(II) and Pd(II) complexes were prepared. The structure of the ligand and its complexes were determined by H-1 NMR, FTIR, UV-Vis, elemental analysis, thermal analyses and magnetic susceptibility spectral data. Further, DNA binding properties of the ligand and its metal complexes were investigated and docking studies were carried out. As a result ligands and copper complex showed DNA binding activity and an increase with H2O2. According to these results it can be suggested that these compounds are very suitable drug candidates. (C) 2020 Elsevier B.V. All rights reserved.