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    EFFECTS OF MANGANESE ETHYLENE-BIS-DITHIOCARBAMATE (MANEB) ON RAT NASAL RESPIRATORY MUCOSA
    (Carbone Editore, 2013) Deveci, E.; Yorgancilar, E.; Ekinci, C.; Koparal, M.; Akkus, Z.; Yavuz, D.
    Maneb (Mn-ethylene(bis)dithiocarbamate is a dithiocarbamate pesticides. It is a fungicide pesticide used in the control of mildew disease on certain crops. In general, dithiocarbamates are considered chemicals of low toxicity for humans. However, the effects of maneb in humans are diverse. It has some effects to humans via absorption through skin and respiratory system. The nasal mucosa is the first part of the airways in contact with the environment and toxic agents. The aim of this study was todetermine the histopathological effects of numb on nasal mucosa. In experimental group, the maneb was administered with inhalation to 10 male Wistar-Albino rats for five days a week. The control group (n=10) received distilled water with spray at the same time period. The experiment was terminated after three weeks. In each case, sections of the nosewere taken. In experimental group,microscopic examination of nasal respiratory mucosa revealed; Hypertrophy of epithelial cells and goblet cells, increasing of mucus secretion, decreasing in sizes of cilia and flattening of the cells near the basement membrane and apoptotic cells in the degenerated respiratory epithelial cells, detected a mild inflammatory reaction and a vascular dilatation in the connective tissue. All changes were statistically significant. In control group of 10 rats no significant histopathologic lesions were found. As a result, our study, maneb is potentially toxic agent to respiratory mucosa.
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    Effects of Melatonin on Tibia Bone Defects in Rats
    (Soc Chilena Anatomia, 2016) Koparal, M.; Irtegun, S.; Alan, H.; Deveci, E.; Gulsun, B.; Pektanc, G.
    The aim of this study was to evaluate the effects of melatonin healing in a tibial bone defect model in rats by means of histopathological and immunohistochemistry analysis. Twenty one male Wistar albino rats were used in this study. In each animal, bone defects (6 mm length) were created in the tibias. The animals were divided into three groups. In group 1 control group (rats which tibial defects). Group 2 melatonin (10 mg/kg) + 14 days in the tibial defect group) was administered intraperitoneally to rats. Group 3 melatonin (10 mg/kg) + 28 days in the tibial defect group) was administered intraperitoneally to rats. Histopathological analysis of samples was performed to evaluate the process of osteoblastic activity, matrix formation, trabecular bone formation and myeloid tissue in bone defects. Immunohistochemical and immunoblot analysis demonstrated non-collagenous proteins (osteopontin and osteonectin) differences in tibial bone defects. The expression of osteopontin on tibia was increased by 14 days melatonin treatment. The expression of osteonectin on tibia was dramatically increased by 14 days melatonin treatment.
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    Experimental investigation of relationship between trauma and bisphosphonate-related osteonecrosis
    (MEDKNOW PUBLICATIONS & MEDIA PVT LTD, B-9, KANARA BUSINESS CENTRE, OFF LINK RD, GHAKTOPAR-E, MUMBAI, 400075, INDIA, 2014) Ağaçayak, K. S.; Yüksel, H.; Atılgan, S.; Koparal, M.; Uçan, M. C.; Özgöz, M.; Yaman, F.; Atalay, Y.; Acıkan, I.
    Background: Bisphosphonate (BP)-related osteonecrosis of the jaw (BRONJ) disease is rare, but there are serious side-effects of BP therapy in patients. In some patients, surgery is needed and could not be cured. A standard test is not available showing the risk of jaw osteonecrosis in routine use. The measurement of serum C-terminal telopeptide (CTX) levels has been used in diseases of BRONJ resorption and antiresorptive therapy. Aim: This paper is aimed at investigating the relationship between traumatic procedures and presence of BP-related osteonecrosis. Materials and Methods: Thirty male Wistar albino rats with weighing 200 +/- 20 g were used for the experimental procedures. Rats were randomly divided into three groups each containing 10 rats as follows: Group 1 (traumatic extraction group), Group 2 (atraumatic extraction group), and Group 3 (control group). All groups, zoledronic acid (ZA) (0.3 mg/kg/week)([1]) was diluted with physiological saline and given subcutaneously for 2 months. After the 2 months, Group 1 was subjected to traumatic extraction of right first lower molars, and Group 2 was subjected to atraumatic extractions of the right first lower molars. Group 3 was subjected to no extractions as a control group. Animals were euthanized 32 days after tooth extractions, and the ZA administration protocol was maintained until the animals' death. After sacrifice, blood samples were collected for C-terminal cross-linking telopeptide of type I collagen (CTX-1) levels, clinical and radiological findings were recorded. Results: The bone resorption marker CTX-1 showed a significant difference among the groups. CTX-1 was measured significantly higher in blood samples of Group 2 (4.15 +/- 0.34; P = 0.001) than Group 1 (3.77 +/- 0.34; P = 0.0001). No, statistically significant changes were found between Groups 1 and 2 as for clinical and radiological assessment. Conclusion: This study provides preliminary observations for the development of an animal model of BRONJ. Although clinical and radiological findings were not relevant, serum CTX values are reliable biochemical markers for predicting BRONJ and also atraumatic surgical procedures are important to prevent BRONJ.
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    Influence of caffeic acid phenethyl ester on bone healing in a rat model
    (Sage Publications Ltd, 2013) Ucan, M. C.; Koparal, M.; Agacayak, S.; Gunay, A.; Ozgoz, M.; Atilgan, S.; Yaman, F.
    Objective To examine the effects of caffeic acid phenethyl ester (CAPE; a component of honey bee-hive propolis with antioxidant, anti-inFLammatory, antiviral and anticancer properties) on bone regeneration and fibrotic healing in a rat model. Methods Male Sprague-Dawley rats (n=63; mean age 7 weeks; weight 280-490g) were randomly divided into three groups: A, cranial defect with no bone healing treatment (n=21); B, cranial defect treated with CAPE (n=21); C, cranial defect treated with CAPE and -tricalcium phosphate/hydroxyl apatite (n=21). Rats were anaesthetized with ketamine (8mg/100g) by intraperitoneal injection and a cranial critical size bone defect was created. Following surgery, CAPE (10 mu mol/kg) was administered by daily intraperitoneal injection. Seven rats in each group were killed at days 7, 15 and 30 following surgery. Bone regeneration, fibrotic healing and osteoblast activity were evaluated by histopathology. Results Statistically significant differences in healing were found between all groups. There were no statistically significant within-group differences between day 7 and 15. At day 30, bone healing scores were significantly higher in groups B and C compared with group A. Conclusion CAPE significantly improved bone-defect healing in a rat model, suggesting that CAPE has beneficial effects on bone healing.