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    Biochemical and histopathological investigation of resveratrol, gliclazide, and losartan protective effects on renal damage in a diabetic rat model
    (Science Printers and Publishers Inc., 2015) Ezel T.; Kocyigit Y.; Deveci E.; Atamer Y.; Sermet A.; Uysal E.; Aktaş A.
    OBJECTIVE: To compare the protective effects of resveratrol, gliclazide, and losartan, at biochemical and histopathological levels, on the rat kidney with experimentally induced type 1 diabetes. STUDY DESIGN: A total of 35 adult male Wistar rats were divided into control, diabetic, diabetic gliclazide, diabetic resveratrol, and diabetic losartan groups. For biochemical analysis, based on one of the kidneys, superoxide dismutase, malondialdehyde, and catalase were used for measurement. The other kidney was stained for histochemical and immunohistochemical markers and examined by light microscopy. RESULTS: Nephropathy due to diabetes was developed at the end of the third week in the diabetic group: in the glomeruli, contraction from Bowman distance, diffuse mesangial matrix increasing and tubular dilation, and cytoplasmic vacuolar changes were observed. In tubulointerstitial areas, some tubular structures, an increased expression of VEGF was observed. CONCLUSION: As a result, in diabetic rats, the effects of gliclazide, resveratrol, and losartan cure were equivalent to each other according to the parameters which were followed. Resveratrol, gliclazide, and losartan significantly protected renal glomeruli and the proximal and distal tubules. © Science Printers and Publishers, Inc.
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    Öğe
    Insulin regulates plasma ghrelin concentrations in streptozotocin-induced diabetic rats
    (University of Dicle, 2014) Tasdemir E.; Obay B.D.; Bilgin H.M.; Sermet A.; Yildirim Y.; Kocyigit Y.
    Ghrelin, an orexigenic peptide produced in the stomach has shown to elicit peripheral actions including regulation of pancreatic ß-cell function. The aim of the study is to clarify the regulation of plasma ghrelin concentrations by insulin in streptozotocin (STZ) induced diabetic rats. Adult male Wistar rats were divided into control and three experimental groups as each group had 7 rats (n=28). To investigate the role of ghrelin in the hyperphagic response to uncontrolled diabetes, experimental groups of rats were injected once daily for 7 days with either STZ (70 mg/kg i.p.) or insulin subcutaneously (5-7 U). Plasma insulin, ghrelin and glucose concentrations were measured. STZ-induced diabetic rats were markedly hyperphagic as accompanied by hyperglycemia. Treatment of diabetic rats with insulin reversed these changes. STZ- induced diabetic rats had higher plasma ghrelin concentrations than control rats. Ghrelin levels were attenuated by the subcutaneous injection of insulin (5-7 U over 7 days). Insulin treatment also partially reversed hyperphagia observed in STZ- induced diabetic rats and there was a decrease in plasma ghrelin concentrations compared with STZ-INS pair fed rat. The results indicate that insulin treatment reverses elevated plasma ghrelin concentrations in STZ- induced diabetic rats suggesting the pathophysiological significance of ghrelin in diabetes.