Yazar "Khawar K.M." seçeneğine göre listele

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    Direct bulblet regeneration from Sternbergia fischeriana (Herb.) Rupr. bulb scale explants
    (SEJANI Ltd., 2014) Kizil S.; Khawar K.M.; Altuntas C.; Saglam S.
    Attractive golden yellow flowered Sternbergia fischeriana (Herb.) Rupr. multiplies very slow under natural conditions. The study reports multiplication of plants using 0.5, 1.0, and 1.5 cm long bulb scale explants with two, three, four, and five scales attached by a thin base plate segment. Any concentration of 2,4-D 1.0, 2.0, 3.0, 4.0, and 5.0 mg l-1 in MS medium was ineffective to induce bulblet regeneration on any explant at 15° ± 1°C. Discursive induction of one or two 0.1 cm diameter bulblets was noted at 24 ± 1°C on 0.5 cm long twoscale explants. Variable regeneration was observed on 0.5, 1.0, and 1.5 cm long two-scale explants on MS basal medium containing combination of BAP and 0.2 mg l-1 NAA. Maximum number of 5.0 ± 0.5 bulblets per 0.5 cm long two-scale bulb explant was obtained on MS medium containing 8.5 mg l-1 BAP plus 0.20 mg l-1 NAA. The rooting as affected by the size of bulblet was achieved on MS medium containing 0.75 mg l-1 NAA. The highest rooting was recorded on 0.47 cm diameter bulblets with 4.3 ± 0.9 roots per bulblet and 3.7 ± 0.4 cm long roots.
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    Öğe
    Effect of different sucrose and ga3 concentrations on in vitro propagation of fritillaria imperialis L.
    (International Society for Horticultural Science, 2015) Kizila S.; Khawar K.M.
    Fritillaria imperialis L. is an important ornamental plant of economic importance. It can be reproduced both by generative and vegetative means. However, regenerative multiplication of the plant is very difficult due to high seed dormancy. The study aimed to germinate the seeds of F. imperials on MS medium supplemented with combinations of 10-50 g L-1 sucrose and 0.5-2.5 mg L-1 GA3. The maximum percentage of seed germination was noted on MS medium containing 20 g L-1 sucrose. Hundred percent rooting was noted on MS medium containing 10, 30 and 40 g L-1 sucrose. The induced bulbs were transferred to MS medium containing 0.5-2.5 mg L-1 GA3 and 20-30 g L-1 sucrose for the growth and development of bulblets. These bulblets induced roots on MS medium supplemented with 30 g L-1 sucrose and 2.5 mg L-1 GA3. Finally, rooted bulblets were transferred to flower pots for acclimatization.
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    Öğe
    The effects of plant growth regulators and incubation temperatures on germination and bulb formation of Fritillaria persica l.
    (SEJANI Ltd., 2014) Kizil S.; Khawar K.M.
    Fritillaria persica L. with large attractive flowers is native to Western Asian countries of the Middle East including Turkey. Wild populations of this species have shown sharp decrease during last few decades due to habitat destruction for number of socio-economical and anthropological reasons. F. persica is propagated through bulbs and seeds. The seeds have significantly high dormancy such that few seeds germinate under natural conditions. Therefore, this study developed a seed dormancy break protocol using MS medium containing variants of BAP with and without IBA incubated at 4°C in dark. Maximum seed dormancy break (80.00 ± 0.14%) was registered on MS medium enriched with 2.0 mg l-1 BAP plus 1.0 mg l-1 IBA and maximum bulblet induction (40.0 0 ± 0.71) was noted on MS medium containing 1.0 mg l-1 BAP plus 1.0 mg l-1 IBA. Similarly, alternating incubation temperatures of 4° and 10°C for variable durations in days influenced seed germination and bulblet induction variably with 100% seed germination and bulblet induction at 75 days incubation at 4°C followed by 15 days incubation at 10°C. The results also suggested that minimum incubation period of 30 days at 4°C followed by incubation at 10°C for 60 days was required to break seed dormancy. The increase in bulblet diameter was achieved on MS medium containing 50 mg l-1 sucrose by incubating the bulblets at 4°C for 30 days. Rooting of the Fritillaria bulblets was obtained on MS medium enriched with 0.5 mg l-1 NAA. This propagation method could be exploited practically avoiding any seasonal constraints to obtain plant material and suggests a positive step further for in vitro propagation of F. persica.
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    Improved in vitro propagation of Hyacinthus orientalis L. using fruits containing immature zygotic embryos and tender leaf sheath as explants
    (Wydawnictwo Akad Rolniczej W Lublinie, 2016) Kizil S.; Sesiz U.; Khawar K.M.
    Hyacinthus genus is an important group of ornamental plants that bear white, yellow, pink, red or purple coloured flowers. It has about 2000 species spread around the world that are grown commercially. Although, plant occurs naturally in Turkey yet efforts have not been made to adapt it for open field cultivation. There is need to transfer and es-tablish these plants from wild to fields for commercial use through in vitro and ex vitro approaches, that will help local economy profitably. This study reports in vitro culture of Hyacinthus orientalis L. subsp. orientalis; using fruits containing immature zygotic em-bryos cultured on MS medium containing varying concentrations of Thidiazuron (TDZ) with and without 0.2 mg l-1 naphthaleneacetic acid (NAA) supplemented with 20 or 40 g l-1 sucrose. The study also reports induction of bulblets on tender leaf sheaths on MS medi-um containing different concentrations of benzylaminopurine (BAP) + 0.1 mg l-1 NAA supplemented with 30 g l-1 sucrose. The maximum bulblet regeneration (40%) with 31.33 bulblets/explant was noted on MS medium containing 0.15 mg l-1 TDZ supple-mented with 40 g l-1 sucrose. Whereas, the best bulblet regeneration on tender leaf sheath explants was noted on 1.5 mg l-1 BAP + 0.1 mg l-1 NAA with 2.97 bulblets per explant of 0.55 cm bulb diameter and 1.20 leaves per bulblet. These bulblets were cultured singly on MS medium containing 20 mg l-1 GA3 (Gibberellic acid) + 50 g l-1 sucrose and attained a diameter of 0.75-1.00 cm in 30 days time. The bulbs regenerated on both explants were successfully rooted and acclimatised in plant growth chamber using peat moss followed by their transfer to open field conditions. © by Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie, Lublin 2016.