Effect of different sucrose and ga3 concentrations on in vitro propagation of fritillaria imperialis L.

Fritillaria imperialis L. is an important ornamental plant of economic importance. It can be reproduced both by generative and vegetative means. However, regenerative multiplication of the plant is very difficult due to high seed dormancy. The study aimed to germinate the seeds of F. imperials on MS medium supplemented with combinations of 10-50 g L-1 sucrose and 0.5-2.5 mg L-1 GA3. The maximum percentage of seed germination was noted on MS medium containing 20 g L-1 sucrose. Hundred percent rooting was noted on MS medium containing 10, 30 and 40 g L-1 sucrose. The induced bulbs were transferred to MS medium containing 0.5-2.5 mg L-1 GA3 and 20-30 g L-1 sucrose for the growth and development of bulblets. These bulblets induced roots on MS medium supplemented with 30 g L-1 sucrose and 2.5 mg L-1 GA3. Finally, rooted bulblets were transferred to flower pots for acclimatization.