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    The combined effect of rosuvastatin and ischemic pre- or post-conditioning on myocardial ischemia-reperfusion injury in rat heart
    (Verduci Publisher, 2015) Kelle, I.; Akkoc, H.; Uyar, E.; Erdinc, M.; Evliyaoglu, O.; Saribas, S.; Tunik, S.
    OBJECTIVE: To investigate the combined effect of rosuvastatin and ischemic preconditioning or postconditioning on ischemia-reperfusion injury in in vivo rat heart. MATERIALS AND METHODS: Ninenty-six male adult Wistar rats were randomly assigned to eight groups: Sham group, ischemia-reperfusion, rosuvastatin preconditioning, rosuvastatin postconditioning, ischemic preconditioning, ischemic postconditioning, ischemic + pharmacologic preconditioning and ischemic + pharmacologic postconditioning groups. Blood samples were taken for creatine kinase evaluation at selected time points. Six rats in each group were separated for either infarct size assessment or immunohistochemical staining with Bcl-2 antibody. RESULTS: The staining with Bcl-2 was significantly lower in groups Sham, ischemic + pharmacologic preconditioning and ischemic + pharmacologic postconditioning groups which is well correlated with the decrease in infarct size for the same groups. The creatine kinase enzyme levels were also reduced to their lowest levels in ischemic + pharmacologic preconditioning and ischemic + pharmacologic postconditioning groups. CONCLUSIONS: These findings suggest that enriching the composition of reperfusate with rosuvastatin along with ischemic preconditioning or postconditioning procedures at the opposite sides of ischemia may interact synergistically for protecting ischemic myocardium from reperfusion injury. The combined application of rosuvastatin with ischemic preconditioning or ischemic postconditioning may provide a new therapeutic option in clinical interventions when compared to single treatment with ischemic and rosuvastatin preconditioning or postconditioning.
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    EFFECTS OF ETHYL PYRUVATE ON TESTICULAR DAMAGE IN RATS WITH STREPTOZOTOCIN-INDUCED DIABETES
    (Editura Acad Romane, 2012) Akkoc, H.; Kelle, I.; Tunik, S.; Erdinc, M.; Erdinc, L.; Nergiz, Y.
    Diabetes mellitus is associated with impairment of testicular functions. Aim. The present study aimed to investigate the effects of ethyl pyruvate (EP) on the testicular tissue damage in rats with streptozotocin (STZ)-induced diabetes. Subjects and methods. Thirty-two Wistar albino male rats were assigned into four equal groups as follows: (1) control group (n:8); (2) EP-treated non-diabetic group (n:8); (3) diabetic group (n:8); and (4) EP-treated diabetic group (n:8). Rats with STZ-induced diabetes were kept alive for 14 weeks. After that, the EP solution was administered intraperitoneally to the rats in the EP-treated non-diabetic and diabetic groups at the dose of 50 mg/kg twice daily for 14 clays. At the end of this period, the left testes were removed from the rats for malondialdehyde (MDA) analysis, and the right testes were removed for histological examination. Results. As compared with the control group, the diabetic group had elevated MDA levels (210.9 +/- 12.7) and increased thickness of the basement membrane of the seminiferous tubules (3.01 +/- 0.16), but decreased tubular diameter (159 +/- 9.0) and Johnsen's score (5.31 +/- 0.1). In the EP-treated diabetic group, diabetes-induced impairment was significantly improved. Conclusion. These findings indicate that EP shows protective effects against diabetes-induced testicular dysfunction.
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    Protective effect of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) polymerase in distant liver injury induced by renal ischemia-reperfusion in rats
    (Verduci Publisher, 2014) Hekimoglu, A. Tas; Toprak, G.; Akkoc, H.; Evliyaoglu, O.; Tas, T.; Kelle, I.; Colpan, L.
    BACKGROUND AND AIMS: Renal ischemia followed by reperfusion causes remote liver injury. This research was planned to investigate whether 3-aminobenzamide (3-AB), has any preventive effect against distant liver injury triggered by renal IR. MATERIALS AND METHODS: Twenty four rats were randomly divided into three different groups Each group has 8 rats. The groups were as follows: (1) Sham operated group; (2) Renal ischemia-reperfusion (IR) group; (3) Renal IR+3-AB group. 3-AB (10 mg/ kg) was given intraperitoneally 10 minute before reperfusion. At the end of study, the rats were sacrificed. Their liver tissues and serum samples were collected for measurement of malondialdehyde (MDA) levels, total oxidant status (TOS), total antioxidant status (TAS), paraoxonase (PON-1) activity and nitric oxide (NO). RESULTS: Renal IR injury significantly increased Oxidative stress index (OSI) and MDA, TOS levels and significantly decreased PON-1 actvity and TAS, NO levels in serum and liver tissue (p < 0.05). Despite that, changes in these biochemical parameters related with IR injury were diminished by 3-AB administration (p < 0.05). CONCLUSIONS: The inhibition of PARP [Poly(ADP-Ribose) Polymerase] by 3-AB showed protective effects against distant liver injury triggered by renal ischemia-reperfusion by the ameliorating effects of 3-AB on oxidative stress.
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    Protective effect of ethyl pyruvate on liver injury in streptozotocin-induced diabetic rats
    (Univ Catholique Louvain-Ucl, 2012) Akkoc, H.; Kelle, I.; Tunik, S.; Bahceci, S.; Sencar, L.; Ayaz, E.; Nergiz, Y.
    Background and aims : Diabetes Mellitus, leading to an increase in oxidative stress, can cause liver damage. Our aim was to investigate the antioxidant effects of Ethyl Pyruvate (EP) on the liver tissue in diabetic rats. Materials and methods : Thirty-two Wistar albino rats were separated into four equal groups. Groups were assigned as follows : (1) Non-diabetic group; (2) EP-treated non-diabetic group; (3) diabetic group; and (4) EP-treated diabetic group. In order to induce diabetes mellitus, 45 mg/kg b.w. streptozotocin was administered intraperitoneally to the rats in groups 3 and 4. On the 3rd day, blood glucose was assessed. Rats with blood glucose levels higher than 300 mg/dl were considered to be diabetic. The EP solution was administered intraperitoneally at a dose of 50 mg/kg b.w. twice daily for 14 days to the rats in groups 2 and 4. The other rats were simultaneously given the same amount of Ringer's lactate solution intraperitoneally. Liver tissue was obtained for malondialdehyde (MDA) analyses and histopathological examination. Results : In group 4, Total Antioxidant Status (TOS) and MDA levels were significantly lower as compared to group 3. Also, morphological abnormalities occurred in group 3 when compared with non-diabetic groups (groups 1 and 2), whereas the disorders resulting from diabetes improved significantly in group 4. Conclusions : These findings show that EP has protective effects against diabetes-induced liver injury. (Acta gastroenterol. belg, 2012. 75, 336-341).