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    Direct plant regeneration from in vitro-derived leaf explants of Hypericum spectabile, a medicinal plant
    (Academic Journals, 2011) Akbas, Filiz; Isikalan, Cigdem; Namli, Sureyya; Karakus, Pinar; Basaran, Davut
    An efficient and reproducible procedure is described for direct plant regeneration using in vitro regenerated leaf explants of Hypericum spectabile. The leaf explants were cultured on MS media supplemented with six different concentrations (0.25, 0.5, 1.0, 1.5, 2.0, 2.5 mg/L) of BAP and kinetin seperately. All of the BAP concentration, shoot regeneration occurred directly without callus formation, but the number of shoots changed, depending on the different concentrations of BAP. The highest and the lowest number of shoots were obtained on the medium supplemented with 1.0 and 2.5 mg/L of BAP (90 to 50%, respectively). In the present study, the medium containing 0.25, 0.5 and 2.5 mg/L Kin did not promote adventitious shoots formation. Among concentrations of Kin, the best results in terms of both shoots number and morphogenic properties were obtained from MS medium supplemented with 1.0 mg/L Kin (60%). In vitro regenerated plants induced roots on half-strength MS medium containing all the concentrations (0.25, 0.5, 1.0, 2.0 mg/L) of IAA. However, the medium supplemented with 0.5 mg/L IAA (100%) was found to be optimum for inducing root. Rooted 5- to 6-week-old in vitro regenerated plants were transferred into a greenhouse for acclimatization.
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    Öğe
    THE EFFECT OF UV-B ON FATTY ACID CONTENT AND RADICAL SCAVENGING ACTIVITY OF METHANOLIC EXTRACTS FROM Hypericum retusum Aucher GROWN UNDER IN VITRO CONDITIONS
    (Parlar Scientific Publications (P S P), 2013) Isikalan, Cigdem; Karakus, Pinar; Kuru, Ibrahim Selcuk; Celik, Kadir Serdar
    The study was aimed to evaluate the effects of UV-B radiation on fatty acid content and antioxidant activity of a methanolic extract of Hypericum retusum Aucher plantlets grown under in vitro conditions. Therefore, the plantlets were exposed to UV-B radiation for different durations (Ti: 15, T-2: 30, T-3: 45, T-4: 60 min) The UV-B application led to increase in linolenic (42.84%), oleic (6.92%) and linoleic acid (28.48%) in H. retusum. The methanol extracts of plantlets grown in vitro conditions showed the strongest free radical scavenging activities at concentrations of 50, 100, and 300 mu g/mL(-1). It has been found that UV-B radiation and in vitro growth conditions have a significant effect on the accumulation of fatty acids. However, the free radical scavenging ability of H. retusum extract was decreased after UV-B application.
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    Öğe
    INDIRECT SHOOT REGENERATION FROM IN VITRO-DERIVED ROOT AND LEAF EXPLANTS OF HYPERICUM SPECTABILE
    (Parlar Scientific Publications (P S P), 2011) Isikalan, Cigdem; Akbas, Filiz; Namli, Sureyya; Karakus, Pinar
    An applicable regeneration protocol through in vitro indirect organogenesis of Hypericum spectabile was developed. Leaf and root explants taken from in vitro grown seedlings were used as explants for callus induction. The explants were inoculated onto Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of benzilaminopurin (BAP; 0.5, 1.0, 1.5 mg l(-1)) and 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5, 1.0, 2.0 mg l(-1)). Callus initiation was observed in all media evaluated from leaf and root explants after two weeks (about 10-15 days) of culture. The best callus induction was observed in leaf explants in the treatment containing 1.0 mg l(-1) BAP + 2.0 mg l(-1) 2.4-D was compact-granular and green in color, while in the other treatments the calli were yellowish-light green and friable (loose). For shoot formation, calli were transferred into different BAP concentrations (0.25, 0.5, 1.0, 1.5 mg l(-1)) separately. Numerous shoots were obtained all media tested. Although the highest number of shoots produced on medium supplemented with 1.5 mg l(-1) BAP (25.15 shoots per explant), the best result was observed in the treatment containing 0.5 mg l(-1) BAP in terms of shoot development and morphogenic properties. The number of shoots produced was 22.75 per explant in these medium.