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  • Küçük Resim
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    Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC-ESI-MS/MS with chemometric approach
    (Marmara University, 2021) Mokaddem-Daroui, Habiba; Mostefa, Maya Belhadj; Aydoğmuş-Öztürk, Fatma; Erol, Ebru; Öztürk, Mehmet; Ertaş, Abdulselam; Duru, Mehmet Emin; Kabouche, Ahmed; Kabouche, Zahia
    The fractions (Fr.1-5) of the hydromethanolic extract of Abies numidica cones were studied for their polyphenol constituents, antioxidant, and anticholinesterase activities. The beta-carotene-linoleic acid, cupric reducing power (CUPRAC), DPPH scavenging, and ABTS radical scavenging assays were used to determine antioxidant activity. Fr.3 exhibited the highest antioxidant activity in ABTS test (IC50: 1.29 mu g/mL), b-carotene-linoleic acid test (IC50: 18.6 mu g/mL) and CUPRAC (A(0.5): 33.8 mu g/mL) assays. Three fractions (Fr.2-4) promising antioxidant activity were analyzed using LC-MS/MS for their phenolic compositions. Taxifolin (155.9-2816.2 mu g analyte/g extract), hyperoside (353.0-2045.5 mu g/g), vanillin (1488.9-1529.9 mu g/g), tannic acid (1281.6-1416.8 mu g/g), rosmarinic acid (1063.1-1149.3 mu g/g), coumarin (683.0-772.6 mu g/g), quercetin (881.0 mu g/g), and catechin (277.7-400.2 mu g/g) were identified in the antioxidant fractions. Moreover, the anticholinesterase activity was also performed using the in vitro spectroscopic Ellman method. The antioxidant fractions exhibited moderate butyrylcholinesterase inhibitory activity. The multi-ware analysis was performed to understand the origin of bioactivities. According to principal component analysis, it is detected that the hyperoside, catechin, taxifolin, and tannic acid were responsible for the antioxidant activity. Therefore, the cones of Abies numidica can be considered as potent antioxidant and anticholinesterase sources in food and pharmaceutical industries, commercially.
  • [ X ]
    Öğe
    Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LCESI- MS/MS with chemometric approach
    (2021) Duru, Mehmet Emin; Erol, Ebru; Ertaş, Abdulselam; Kabouche, Zahia; Mostefa, Maya Belhadj; Mokaddem-Darouı, Habiba; Kabouche, Ahmed
    The fractions (Fr.1-5) of the hydromethanolic extract of Abies numidica cones were studied for theirpolyphenol constituents, antioxidant, and anticholinesterase activities. The ?-carotene-linoleic acid, cupric reducingpower (CUPRAC), DPPH scavenging, and ABTS radical scavenging assays were used to determine antioxidant activity.Fr.3 exhibited the highest antioxidant activity in ABTS test (IC50: 1.29 ?g/mL), b-carotene-linoleic acid test (IC50: 18.6?g/mL) and CUPRAC (A0.5: 33.8 ?g/mL) assays. Three fractions (Fr.2-4) promising antioxidant activity were analyzedusing LC-MS/MS for their phenolic compositions. Taxifolin (155.9-2816.2 ?g analyte/g extract), hyperoside (353.0-2045.5 ?g/g), vanillin (1488.9-1529.9 ?g/g), tannic acid (1281.6-1416.8 ?g/g), rosmarinic acid (1063.1-1149.3 ?g/g),coumarin (683.0-772.6 ?g/g), quercetin (881.0 ?g/g), and catechin (277.7-400.2 ?g/g) were identified in the antioxidantfractions. Moreover, the anticholinesterase activity was also performed using the in vitro spectroscopic Ellman method.The antioxidant fractions exhibited moderate butyrylcholinesterase inhibitory activity. The multi-ware analysis wasperformed to understand the origin of bioactivities. According to principal component analysis, it is detected that thehyperoside, catechin, taxifolin, and tannic acid were responsible for the antioxidant activity. Therefore, the cones ofAbies numidica can be considered as potent antioxidant and anticholinesterase sources in food and pharmaceuticalindustries, commercially.
  • Küçük Resim
    Öğe
    Phenolic compounds, antioxidant and antibacterial activities of cedrus Atlantica stem barks
    (Springer, 2024) Benmerache, Abbes; Kabouche, Ahmed; Kabouche, Zahia; Öztürk, Mehmet; Çakır, Cansel; Yılmaz, Mustafa Abdullah; Ertaş, Abdulselam
    Stem barks of Cedrus atlantica were investigated for their antioxidant and antibacterial activities and their chemical composition. The phenolic ingredients of the extracts were studied using LC-MS/MS against twenty-seven phenolic compounds. The antioxidant activity was evaluated by DPPH, O-2-DMSO-alkaline, ABTS, beta-carotene-linoleic acid and CUPRAC assays, while the antibacterial activity was performed using the disk diffusion and MIC assays. In addition, the phenolic and flavonoid contents were determined as gallic acid and quercetin equivalents, respectively. Ethyl acetate extract exhibited the highest activity in DPPH, O-2-DMSO alkaline, ABTS, beta-carotene linoleic acid and CUPRAC assays (IC50 = 10.85 +/- 1.41, 3.36 +/- 0.40, 0.74 +/- 0.03, 10.03 +/- 0.01, A(0.50) = 23.56 +/- 1.67 mu g/mL, respectively), followed by n-butanol and hydromethanol extracts. In addition, the ethyl acetate and n-butanol extracts showed remarkable antibacterial activity against Enterobacter aerogenes and Staphylococcus aureus (ATCC 43300), respectively. The LC-MS/MS analyses of all extracts afforded high amounts of quinic acid, malic acid, rosmarinic acid, protocatechuic acid, chlorogenic acid, coumarin, vanillin, and quercetin, exhibiting antioxidant activity.