Yazar "Isik, Birgul" seçeneğine göre listele
Listeleniyor 1 - 8 / 8
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Antioxidant Signal and Kidney Injury Molecule-1 Levels in Shockwave Lithotripsy Induced Kidney Injury(Mary Ann Liebert, Inc, 2014) Hatipoglu, Namik Kemal; Evliyaoglu, Osman; Isik, Birgul; Bodakci, Mehmet Nuri; Bozkurt, Yasar; Sancaktutar, Ahmet Ali; Soylemez, HalukPurpose: Shockwave lithotripsy (SWL) induces acute kidney injury (AKI) that extends from the papilla to the outer cortex by causing ischemia and the production of nephrotoxic agents. Direct ischemic damage and the generation of free radicals cause injury to the proximal tubular cells. Kidney injury molecule-1 (KIM-1) is a transmembrane glycoprotein that is upregulated in proximal tubular cells after ischemic or nephrotoxic injury and is not expressed in healthy kidneys. We evaluated the extent of free radical production in response to SWL by measuring urinary total antioxidant capacity (TAC) and total oxidant status (TOS). Furthermore, we investigated the severity of SWL-induced kidney injury by measuring KIM-1 expression levels. Patients and Methods: The study population comprised 30 patients who were carefully selected and 30 age and sex matched control subjects. All patients received the same SWL procedure. Midstream urine samples were collected from patients before SWL and at 120 minutes after SWL. Urine KIM-1 levels were measured by enzyme-linked immunosorbent assay, and TAC and TOS were measured via spectrophotometry. Results: Mean levels of TAC (2.880.56mmolTxEq/L),TOS (8.27 +/- 1.57molH(2)O(2)Eq/L), and KIM-1 (0.55 +/- 0.08ng/mL) before SWL were not significantly different from mean TAC, TOS, and KIM-1 levels measured from the control group at 2.81 +/- 0.42mmolTxEq/L, 10.73 +/- 1.4molH(2)O(2)Eq/L, and 0.51 +/- 0.07ng/mL, respectively. Two hours after SWL, mean urine TAC levels (2.81 +/- 0.85mmolTxEq/L, P=0.02) were decreased and mean KIM-1 expression (0.85 +/- 0.11ng/mL, P=0.01) was significantly increased, but there was no significant difference in mean TOS levels (11.24 +/- 1.9molH(2)O(2)Eq/L, P=0.627) compared with the control group. Conclusions: The increased burden of free radical oxidants in the setting of decreasing antioxidant capacity may be one of the initial indicators of AKI after SWL. Moreover, KIM-1 demonstrates great potential as an early and noninvasive biomarker of SWL-induced kidney injury.Öğe CHANGES IN SERUM PARAOXONASE ACTIVITY, CALCIUM AND LIPID PROFILES IN PRE-ECLAMPSIA, A PRELIMINARY STUDY(Galenos Yayincilik, 2011) Babacan, Figen; Isik, Birgul; Bingol, BanuObjective: To investigate serum paraoxonase (PON1) activity, serum calcium, and lipid profiles in pre-eclampsia. Material and methods: Serum PON1 activity, calcium, and lipid profiles were measured in a cohort of 45 women with normal pregnancies (11 patients) and pre-eclampsia (34 patients). Results: Triglyceride, total cholesterol, and LDL cholesterol concentrations were significantly increased in women with pre-eclampsia, compared with women with normal pregnancies. Serum HDL cholesterol, PON1, and calcium levels in pre-eclamptic patients were significantly lower than those in healthy pregnant women. Conclusion: The significant decrease of PON1 activity in pre-eclampsia is related to lipid profiles and calcium.Öğe Chemical Characteristics of Settled Particles during a Dust-Storm(Hard, 2012) Aydin, Firat; Aydin, Isil; Erdogan, Sait; Akba, Osman; Isik, Birgul; Hamamci, CandanMajor synoptic systems bring desert dust from Arabia and the Sahara to Anatolia, Turkey. A study of particulate matter (PM) associated metal content such as As, Al, Cr, Cd, Co, Cu, Fe, Mn, Mo, Ni, Pb, Sb, Se, Sn, and Zn, etc., has been carried out for Diyarbakir, SE Anatolia, Turkey. Results show that the PM10 levels during a dust storm were much higher than in a non-dust-storm period and that four metals (Fe, Al, Cr, Mn) were dominant in settled particles. This research does not only provide background data for air quality management, but also provides valuable information about the chemical composition of the particles.Öğe Effect of ELF magnetic fields on lipid peroxidation, sperm count, p53, and trace elements(Int Scientific Literature, Inc, 2006) Akdag, M. Zulkuf; Dasdag, Suleyman; Aksen, Feyzan; Isik, Birgul; Yilmaz, FahriBackground: Some epidemiological and laboratory studies suggest a possible connection between extremely low-frequency (ELF) magnetic fields and certain illnesses, such as cancer, immune suppression, as well as reproductive toxic effects and abnormalities. Therefore, the aim of this study was to investigate the effects of ELF magnetic fields (1.35 mT) on sperm count, malondialdelivde concentration, the histology of such organs as the testes, brain, liver, and kidney tissues, p53 immunoreactivity of bone marrow, and the serum concentrations Of Cu2+, Zn2+, Mn2+, and Fe3+ in rats. Material/Methods: Sixteen Sprague-Dawley male rats were divided into two groups. The rats in the experimental group were exposed to an ELF magnetic field 2 hr/day for 2 months (7 days a week). The rats in the control group were not exposed to the ELF magnetic field. The exposure was performed in a Faraday cake (130x65x80 cm) with grounded shielding against the electric component. The Mann-Whitney U-test was used for the statistical analysis of the data. Results: Magnetic field measurements showed that, under the experimental conditions, the magnetic field-exposure system produced a stable. flux density of 1.35 +/- 0.018 mT and a stable frequency of 50 Hz, with negligible harmonics and no transients. However, no statistically significant alteration was observed in the parameters measured in this study except in Mn2+ concentrations (p < 0.001). Conclusions: The present study found no evidence of an adverse effect of ELF magnetic fields on the measured parameters except for significantly increased Mn2+ concentrations (p < 0.001).Öğe An Evaluation of Nitric Oxide, Folate, Homocysteine Levels and Lipid Peroxidation in Postmenopausal Osteoporosis(Wroclaw Medical Univ, 2013) Akpolat, Veysi; Bilgin, H. Murat; Celik, M. Yusuf; Erdemoglu, Mahmut; Isik, BirgulObjectives. In the current study, the risk coefficients of nitric oxide (NO), folate, homocysteine levels and lipid peroxidation in postmenopausal osteoporotic women were determined. Material and Methods. Bone mineral density was determined by dual-energy X-ray absorptiometry. The levels of serum lipid peroxidation products such as the thiobarbituric acid adduct of malondialdehyde (MDA) were measured spectrophotometrically. Plasma folate and homocysteine (Hcy) levels were measured by enzyme chemiluminescence immunoassay. Plasma nitrite levels were measured with the Griess reaction Results. The odds ratios and 95% Confidence Intervals (CI) of the variables MDA, folate, NO, body-mass index (BMI), menopause age and age were found significant. MDA, NO and folate variables were found statistically significant in the analysis of receiver operating charecteristic (ROCs). The areas under the curve (AUCs, 95% CI) of MDA, NO and folate were found to be statistically significant. Conclusions. The current study indicates that NO, MDA and folate are risk variables for postmenopausal osteoporosisÖğe THE EVALUATION OF PARAOXONASE 1 ACTIVITY IN PATIENTS WITH GESTATIONAL DIABETES(Carbone Editore, 2013) Atay, Ahmet Engin; Sakar, Mehmet Nafi; Culcu, Nurdagul Serife Nurani; Simsek, Hakki; Akbas, Halit; Acar, Murat; Isik, BirgulMaterial and methods: Sixty five patients with GDM and 66 healthy pregnants were enrolled. Paraoxonase activity, insulin levels, HOMA-IR, demographic features and anthropometric measurements were evaluated. Results: The mean paraoxonase activity was significantly diminished in patients with GDM (p:0,004). Insulin level and HOMA-IR were significantly higher in GDM patients (p:0,004 and p:0,001; respectively). The mean interval between present and previous pregnancy was significantly shorter in patients group (p:0,004). There was a significant correlation between PON1 activity and serum LDL, HDL levels and weight gained during pregnancy (p:0,001,p<0,001 and p:0,002; respectively) but not with weight and parity. However HOMA-IR was significantly correlated with weight and parity (p:0,001 and p:0,002; respectively). Discussion: Diminished PON1 activity and enhanced oxidative stress accompany to insulin resistance in the pathogenesis of GDM. Oxidative stress is associated with weight gained during first 2 trimesters of pregnancy rather than obesity.Öğe Oxidative stress in smokers and non-smokers(Taylor & Francis Ltd, 2007) Isik, Birgul; Ceylan, Ali; Isik, RecepOxidative stress plays an important role in the pathogenesis of some diseases such as lung cancer, chronic obstructive pulmonary disease, and atheroscleorosis. Smoking may enhance oxidative stress not only through the production of reactive oxygen radicals in smoke but also through weakening of the antioxidant defense systems. In this study, we investigated the effects of smoking on lipid peroxidation and paraoxonase activity in a healthy population. The study consisted of (n = 30) smokers and (n = 30) nonsmokers. The age of the population which is studied was 44.74 +/- 10.59 yr. The levels of serum malondialdehyde (MDA) and paraoxonase (PON1) activities were measured by the modified Buege method and the Eckerson method, respectively. Student's t-test was used to analyze the data. The serum MDA levels were significantly higher (p <.05) and serum PON1 activities were significantly lower (p <.001) in smokers than in nonsmokers. Thus, increased levels of serum MDA and decreased PON1 activities may be important in determining the oxidant/ antioxidant imbalance in smokers.Öğe Serum levels of malonyldialdehyde (MDA) and paraoxonase activities in patients with Behcet's disease(Pergamon-Elsevier Science Ltd, 2009) Isik, Birgul; Aytekin, Sema; Balci, Gokcen[Abstract Not Available]
