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Öğe Determination and assesment of the sex chromosomes of male trees of pistachio (Pistacia vera L.) using in vitro culture(2011) Tilkat E.A.; Namli S.; Işikalan Ç.The chromosome number and karyotype analysis of male trees of Pistacia vera L. was investigated to determine the sex chromosomes. For karyological determination, root tips were obtained from in vitro derived shoots. Afterwards fifty plates of mitosis were used for the karyotyping, the five best metaphase plates were measured for karyotype analysis, karyogram and idiogram. The conclusions obtained from the chromosome studies illustrated that the chromosome complement of P.vera L. has 2n = 30 chromosome pairs with metacentric, submetacentric, subtelocentric and telocentric centromeres. Karyotype analysis revealed that male trees of P.vera L. have heteromorphic sex chromosomes (Xy). The largest pair of chromosomes have different physical features from the other pairs. Neither polyploidy nor satellite was detected on any of these chromosome plates.Öğe Effect of UV-C on production of hypericin in Hypericum triquetrifolium turra grown under in-vitro conditions(2009) Namli S.; Toker Z.; Işikalan Ç.; Özen H.Ç.In this study, the effects of UV-C radiation on the increasing of hypericin in Hypericum triquetrifolium Turra were investigated by using in vitro techniques. The mature seeds of Hypericum triquetrifolium Turra were germinated in Murashige and Skoog (MS) medium without hormone under in vitro conditions, and the shoots obtained were successfully reproduced in MS medium supplemented with 0.25 mg L-1 BA (N6-benzylaminopurine). The UV-C radiation was applied to Hypericum triquetrifolium Turra at different periods (15, 30, 45, 60 min). Although the hypericin content increased at 15-min UV-C radiation, it decreased after 60 min of application. Depending upon different parameters of UV-C radiation, some morphological alterations were observed in shoots.
