Effect of UV-C on production of hypericin in Hypericum triquetrifolium turra grown under in-vitro conditions

In this study, the effects of UV-C radiation on the increasing of hypericin in Hypericum triquetrifolium Turra were investigated by using in vitro techniques. The mature seeds of Hypericum triquetrifolium Turra were germinated in Murashige and Skoog (MS) medium without hormone under in vitro conditions, and the shoots obtained were successfully reproduced in MS medium supplemented with 0.25 mg L-1 BA (N6-benzylaminopurine). The UV-C radiation was applied to Hypericum triquetrifolium Turra at different periods (15, 30, 45, 60 min). Although the hypericin content increased at 15-min UV-C radiation, it decreased after 60 min of application. Depending upon different parameters of UV-C radiation, some morphological alterations were observed in shoots.