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Bioapplications of polythiophene-g-polyphenylalanine-covered surfaces
(Wiley-V C H Verlag Gmbh, 2015) Güler, Emine; Akbulut, Hüseyin; Bozokalfa, Guliz; Demir, Bilal; Eyrilmez, Gizem Oyman; Yavuz, Murat; Demirkol, Dilek Odacı; 0000-0003-3452-8551; 0000-0003-2381-9775; 0000-0003-1642-3481
The fabrication of electro and bioactive surfaces by electrochemical deposition of the thiophene-functionalized polyphenylalanine macromonomer (T-g-PPhe) is reported. The resulting conducting graft copolymer, polythiophene-graft-polyphenylalanine (PT-g-PPhe) formed on the indium tin oxide (ITO) glass surface, is characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and fluorescence microscopy. Then, possible uses of PT-g-PPhe as matrices in the sensor design for both electrochemical biosensing and cell adhesion studies are investigated. In the first part, PT-g-PPhe that is deposited on ITO is further functionalized with the arginylglycylaspartic acid peptide via 1-Ethyl-3-(3 dimethylaminopropyl) carbodiimide for the selective cell adhesion. Immunofluorescence staining is performed to detect the difference between adherences of integrin alpha v beta 3 receptor positive (U87-MG) and negative (HaCaT) cell lines on to the biofunctional surface. In the second part, an electrochemical glucose sensor is constructed by immobilizing glucose oxidase on the surface of PT-g-PPhe, which is deposited on a glassy carbon electrode.