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Öğe Antimicrobial and Antioxidant Activities of Different Extracts of Helichrysum arenarium subsp. (L.) Moench aucheri(2023) Eren, Ayşe; İnci, Şule; Saleh, Kochar Khasro; Kırbağ, Sevda; Güven, KemalHelichrysum arenarium (L.) Moench subsp. aucheri is a herbaceous perennial herb belonging to the Asteraceae. This plant has biological activities such as antibacterial, antiviral, anti-inflammatory, antifungal, antiproliferative, antioxidant, and antiradical. In this study, antimicrobial and antioxidant activities of methanol and ethanol extracts of aerial parts of H. arenarium subsp. aucheri were investigated. To determine the antimicrobial activity pathogenic microorganisms Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus megaterium, Candida glabrata, Candida albicans and Trichophyton sp. Antioxidant activity was determined with total antioxidant value (TAS), total oxidant value (TOS) and 2.2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging capacity. In the results obtained, it was determined that the methanol extract had an antimicrobial effect (9.3 mm) only against C. albicans. It was found that the ethanol extract showed antimicrobial activity at different rates (8.8-20.4 mm) against S. aureus, B. megaterium, C. glabrata, C. albicans and Trichophyton sp. The TAS value of the methanol extract was 3.00 mmol, and the TAS value of the ethanol extract was 3.15 mmol. The TOS value of the methanol extract of the same species was calculated as 6.81 μmol, and the TOS value of the ethanol extract was calculated as 12.64 μmol. The DPPH radical scavenging effects of extracts of goldengrass was found to increase depend on concentrations.Öğe Antimicrobial and Antioxidant Activities of Different Extracts of Helichrysum arenarium subsp. (L.) Moench aucheri(Fırat University, 2023) Eren, Ayşe; İnci, Şule; Saleh, Kochar Kh.; Kırbağ, Sevda; Güven, KemalHelichrysum arenarium (L.) Moench subsp. aucheri is a herbaceous perennial herb belonging to the Asteraceae. This plant has biological activities such as antibacterial, antiviral, anti-inflammatory, antifungal, antiproliferative, antioxidant, and antiradical. In this study, antimicrobial and antioxidant activities of methanol and ethanol extracts of aerial parts of H. arenarium subsp. aucheri were investigated. To determine the antimicrobial activity pathogenic microorganisms Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus megaterium, Candida glabrata, Candida albicans and Trichophyton sp. Antioxidant activity was determined with total antioxidant value (TAS), total oxidant value (TOS) and 2.2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging capacity. In the results obtained, it was determined that the methanol extract had an antimicrobial effect (9.3 mm) only against C. albicans. It was found that the ethanol extract showed antimicrobial activity at different rates (8.8-20.4 mm) against S. aureus, B. megaterium, C. glabrata, C. albicans and Trichophyton sp. The TAS value of the methanol extract was 3.00 mmol, and the TAS value of the ethanol extract was 3.15 mmol. The TOS value of the methanol extract of the same species was calculated as 6.81 µmol, and the TOS value of the ethanol extract was calculated as 12.64 µmol. The DPPH radical scavenging effects of extracts of goldengrass was found to increase depend on concentrations.Öğe Antimicrobial and antioxidant effect of Ficaria verna Huds.(Yüzüncü Yıl Üniversitesi Ziraat Fakültesi, 2021) İnci, Şule; Eren, Ayşe; Kırbağ, Sevda; Özkan, Ahmet İsmailFicaria verna Huds. is a plant belonging to the Ranunculaceae family, known as mole grass and celandine among the people. It is known to have antiinflammatory and anti-haemorrhagic pharmaceutical effects. In this study, it was aimed to determine the antimicrobial effect of different concentrations of F. verna extracts obtained from methanol, ethanol and chloroform and the antioxidant activity of different concentrations of the extract obtained from methanol. In the results obtained, the best antimicrobial effect (17-20 mm) against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Bacillus megaterium, Salmonella thypii and Candida albicans was determined in the methanol extract of F. verna at a concentration of 1000 µg. It was observed that the scavenging effect of the DPPH radical of F. verna increased depending on increasing concentrations.Öğe The Antimicrobial and Antioxidant Effects of Equisetum arvense Extracts(2024) Eren, Ayşe; İnci, Şule; Kırbağ, SevdaEquisetum arvense L, also known as horsetail, is a medicinal plant used in traditional medicine. Especially, it is used in the treatment of bleeding, antiseptic, anti-inflammatory, urethritis, jaundice and hepatitis. In the study, the antimicrobial and antioxidant activities of extracts obtained from different solvents of E. arvense were investigated. Antimicrobial activity of E. arvense extracts was determined using the disc diffusion method. The antimicrobial activity was determined utilizing the pathogenic microorganisms Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, Candida albicans and Candida glabrata. In the results obtained, it was determined that the ethanol extract of E. arvense at 500 µg concentration showed antimicrobial activity at different rates (14.3-28.0). Ethanol extract showed the highest antimicrobial activity against Candida glabrata (28.0 mm) at the same concentration. It was detected that the chloroform extract showed antimicrobial activity (7.3-10.6 mm) against the microorganisms used. The antioxidant activity of the aerial parts of E. arvense at different concentrations of methanol extract was determined according to the 2,2-diphenyl-1-picrylhydrazil radical scavenging capacity method. The highest radical scavenging capacity of the methanol extract was observed at a concentration of 10mg/mL (91.5%). The IC50 value of the methanol extract of E. arvense was calculated as 3.13 mg/mL.Öğe Determination of Antimicrobial and Antioxidant Activity of Alchemilla alpina L.(2021) Kırbağ, Sevda; İnci, Şule; Eren, AyşeAlchemilla genus, which belongs to the Rosaceae family, is a medicinal plant used for various purposes among the people. Species of this genus are known in Turkish folk medicine as lion claw or hazelnut grass. Especially, they are used mainly women’s illnesses, in gastritis, anti- inflammatory, as carminative, and in the treatment of wound. Besides the antimutagenic effect of Alchemilla alpina L., its above-ground parts are used for antimycotic purposes in the form of tea or oral care water. In this study, it has been aimed to determine the antimicrobial effect of the above- ground parts of Alchemilla alpina extracts obtained from methanol, ethanol and chloroform and the antioxidant activity of different concentrations of the extract obtained from methanol. The antimicrobial activity of methanol, ethanol and chloroform extracts of the above-ground parts of A. alpina has been determined according to disk disc diffusion method. In the results obtained have been showed that these extracts inhibited the growth of some bacteria (Staphylococcus aureus ATCC25923, Escherichia coli ATCC25322, Klebsiella pneumoniae ATCC700603, Bacillus megaterium DSM32) and yeasts (Candida albicans FMC17 and Candida glabrata ATCC66032) at different rates (8-23 mm). The antioxidant activity of different concentrations (1.25, 2.5, 5 and 10 mg/ml) of the above-ground parts of A. alpina extract obtained from methanol has been determined according to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity method. In the results obtained, it has been observed that the effect of removing DPPH radical of A. alpina increased depending on increasing concentrations.Öğe Equisetum arvense Ekstraktlarının Antimikrobiyal ve Antioksidan Etkileri(Fırat Üniversitesi, 2024) Eren, Ayşe; İnci, Şule; Kırbağ, SevdaAtkuyruğu olarak bilinen Equisetum arvense L. geleneksel tıpta kullanılan tıbbi bir bitkidir. Özellikle; kanama, antiseptik, antiinflamatuar, üretrit, sarılık ve hepatit tedavisinde kullanılır. Çalışmada E. arvense'nin farklı solventlerinden elde edilen ekstraktların antimikrobiyal ve antioksidan aktiviteleri araştırılmıştır. E. arvense ekstraktlarının antimikrobiyal aktivitesi disk difüzyon yöntemi kullanılarak tespit edilmiştir. Antimikrobiyal aktivite patojenik mikroorganizmalar Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, Candida albicans ve Candida glabrata kullanılarak belirlendi. Elde edilen sonuçlarda E. arvense’nin 500 µg konsantrasonda etanol ekstraktının farklı oranlarda (14,3-28,0) antimikrobiyal aktivite gösterdiği belirlendi. Etanol ekstraktı aynı konsantrasyonda Candida glabrata'ya (28,0 mm) karşı en yüksek antimikrobiyal aktivite göstermiştir. Kloroform ekstraktının kullanılan mikroorganizmalara karşı antimikrobiyal aktivite gösterdiği (7,3-10,6 mm) tespit edilmiştir. E. arvense'nin toprak üstü kısımlarının metanol ekstraktının farklı konsantrasyonlarındaki antioksidan aktivitesi, 2,2-diphenyl-1-picrilhydrazyl radikal temizleme kapasitesi yöntemine göre tespit edilmiştir. Metanol ekstraktının en yüksek radikal temizleme kapasitesi 10mg/mL (%91,5) konsantrasyonda görülmüştür. E. arvense’nin metanol ekstraktının IC50 değeri 3,13 mg/mL olarak hesaplandı.Öğe Isolation and characterization of alkane hydrocarbons-degrading Delftia tsuruhatensis strain D9 from petroleum-contaminated soils(Field Crops Central Research Institute, 2022) Eren, Ayşe; Güven, KemalA bacterial strain from petroleum-contaminated soil in south-eastern Turkey was isolated and characterized to determine the potential of alkane hydrocarbon biodegradation. Phenotypic characteristics and the sequence analysis of the 16S rRNA gene revealed that the strain D9 is a member of the Delfitia genus and most similar to Delftia tsuruhatensis (100%). The optimum pH and temperature values for the growth of D. tsuruhatensis strain D9 were found to be 9.0-10.0 and 35°C, respectively. The strain was found to grow in some single, medium and long-chain hydrocarbons such as decane, hexadecane, and squalene, tested by short-time incubation in basal medium (BM) in the presence of 1% hydrocarbon concentrations under optimum conditions. After incubation for 3 days, 65% of the single hydrocarbon hexadecane was degraded by the D. tsuruhatensis strain D9, revealed by GC-MS analysis. The biodegradation of petroleum hydrocarbons by D. tsuruhatensis strain D9 isolated and characterized in the present study shows that it can be a good candidate in the bioremediation process.Öğe Isolation and characterization of alkane hydrocarbons-degrading enterobacter ludwigii strain D8 from petroleum-contaminated soils(Bitlis Eren Üniversitesi, 2022) Eren, Ayşe; Güven, KemalA bacterial strain has been isolated from petroleum contaminated soil with in southeastern Turkey. This isolated strain was characterized to determine its hydrocarbon biodegradation potential. Phenotypic features and of 16 S gene sequence analysis of rRNA revealed that strain D8 belongs to the Enterobacter genus and most closely resembles Enterobacter ludwigii (100%). The optimum temperature and pH values for the growth of E. ludwigii D8 were found to be 30°C and 5.0, respectively. This bacterial strain grew in long and medium chain hydrocarbons such as 1% decane, pentadecane and squalene separately at the end of 3 day incubation in the basal medium (BM) under optimum conditions. It was shown that E. ludwigii strain D8 degrades about 27% of crude oil incubated for 5 days, while it degrades 29% of pentadecane after 3 days of incubation determined by Gas chromatography-MS analysis. The biodegradation potential of petroleum hydrocarbons of E. ludwigii strain D8 isolated and characterized in this study indicates that this strain may play a role in the bioremediation process.Öğe PCR-based detection of alkane monooxygenase genes in the hydrocarbon and crude oil-degrading Acinetobacter strains from petroleum-contaminated soils(Serbian Chemical Society, 2024) Eren, Ayşe; Bekler, Fatma Matpan; Güven, KemalBacterial strains D11, E1 and E2 isolated from petroleum-contaminated soils were found to be members of Acinetobacter genus revealed by 16S rRNA gene sequence analysis and phenotypic characteristics. After incubation for 5 days, about 43, 9 and 12 % of total petroleum hydrocarbons of crude oil were degraded by strains D11, E1 and E2, respectively, and determined by GC-MS analysis. Moreover, about 70 and 76 % of single hydrocarbon hexadecane was degraded by the strains D11 and E1 after 3 days of short incubation time, respectively, while the strain E2 degraded about 48 % of single hydrocarbon pentadecane. By using PCR-based method, gene sequences of the strains D11 and E2 showed similarity to alkane 1-monooxygenases from Acinetobacter sp. BUU8 alkM with 93.06 and 92.72 %, respectively, while the sequence similarity of strain E1 was 95.84 % to Acinetobacter sp. 826659. The present study of hydrocarbon biodegradation by Acinetobacter strains may provide a good advantage in bioremediation process. © 2024 Physical Society of Japan. All rights reserved.Öğe Petrol ve hidrokarbon parçalayan bakterilerin izolasyonu ve biyoteknolojik potansiyellerinin belirlenmesi(Dicle Üniversitesi, Fen Bilimleri Enstitüsü, 2022) Eren, Ayşe; Güven, KemalPetrol dünyadaki en yaygın çevresel kirleticilerdir. Petrol kirleticileri balıkçılık, deniz habitatları, halobiyolar ve insan sağlığı için ciddi bir tehdit oluşturmakta ve yıllarca hatta on yıllarca sürebilen ekolojik dengeyi tahrip etmektedir. Bu çalışmada, Batman bölgesinde petrolle kirletilmiş alanlardan bakteri izole ederek, petrol parçalayan bu bakteri varyeteler üzerinde morfolojik, fizyolojik, biyokimyasal ve filogenetik analizler yapılarak tanımlanmıştır. Ayrıca, bu bakterilerin ham petrolü ve çeşitli hidrokarbonları enerji kaynağı olarak kullanabilme kapasiteleri, hidrokarbonları parçalama özellikleri ve biyoparçalamada görev alan genlerin tespit edilmesi üzerinde çalışmalar gerçekleştirildi. Batman petrol sahalarından petrol ile kirlenmiş bölgelerden Pseudomonas, Enterobacter, Delftia, Acinetobacter cinslerine ait 8 bakteri izole edilmiştir. Bu izole edilen bakterilerin fenotipik analizleri yapılmış ve 16S rRNA gen dizi analizine göre, sırasıyla D7, D8, D9, D10, D11, D12, E1 ve E2 olarak adlandırılan bakterilerin Pseudomonas aeruginosa'ya %100, Enterobacter ludwigii'ye %100, Delftia tsuruhatensis'e %100, Pseudomonas putida'ya %100, Acinetobacter pittii'ye %99,93, Pseudomonas rhodesiae'ye %100, Acinetobacter pittii'ye %100, Acinetobacter calcoaceticus'a %100 benzer oldukları belirlenmiştir. Çalışılan tüm bakteri suşlarının ham petrolü karbon ve enerji kaynağı olarak kullandıkları belirlenmiştir. Bakterilerin farklı uzunluklara sahip alifatik hidrokarbonlardan, sadece uzun zincirli n-alkanlarda (pentadekan, hekzadekan) ve dekanda iyi ürediği görülmüştür. Test edilen uzun zincirli alkanlar arasında, genel olarak bakteriler tarafından en çok tercih edilen hekzadekan olmuştur. Beş günlük inkübasyon sonucunda GC-MS analizi yapılarak, ham petrolü parçalama potansiyeli açısından yüksekten düşüğe doğru sıralayacak olursak; D11'in %43, D8'in % 27, D12'nin % 21, D10'nun %18, D9'un %12, E2 'nin ise %12 ve E1'in %9 oranında degradasyona uğrattıkları tespit edildi. Üç günlük inkübasyon sonucunda bakterilerin %1'lik alkan hidrokarbonlarını parçalama oranları GC-MS analizi ile belirlenmiştir. Hekzadekanı, D9'un yaklaşık olarak %65, D11'in %70, D12'nin %38, E1'in %76 oranında parçaladığı tespit edilmiştir. Pentadekanı, D7'in %3, D8'in %29, E2'in ise %48 oranında parçaladığı tespit edilmiştir. Ayrıca, üç günlük inkübasyon sonucunda, bakterilerin tek veya karışım halinde hekzadekan ve pentadekandan oluşan hidrokarbon karışımlarını (toplam %1) parçalama oranları belirlenmiştir. Bireysel olarak D8 %31, D11 %43, E2 %53 oranında etkili olurken, karışım halinde D8+D11 %60, D11+E2 %49, D8+E2 %61 ve D8+D11+E2 ise %72 gibi yüksek oranda biyodegradasyon yaptıkları tespit edildi. D11 ve E2 suşları için tasarlanan primerlerden ilki (primer 1) kullanılarak elde edilen PCR ürünlerinin alkM geninin dizi analizi sonuçlarına göre sırasıyla %93,06 ve %92,72 oranında Acinetobacter sp. BUU8 (A6N7F9) alkM gen dizisine benzerlik gösterdiği, ayrıca D11 ve E2 suşları için tasarlanan diğer primer (primer 3) kullanıldığında elde edilen PCR ürünlerinin alkB geni dizi analizi sonuçlarına göre, sırasıyla %94,48 oranında Psudomonas citronellolis (A0A024A3Z2) ve %95,52 oranında Pseudomonas mendocina (A4XPE8) alkB gen dizilerine benzerlik gösterdiği belirlenmiştir.
