Equisetum arvense Ekstraktlarının Antimikrobiyal ve Antioksidan Etkileri
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Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Fırat Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Atkuyruğu olarak bilinen Equisetum arvense L. geleneksel tıpta kullanılan tıbbi bir bitkidir. Özellikle; kanama, antiseptik, antiinflamatuar, üretrit, sarılık ve hepatit tedavisinde kullanılır. Çalışmada E. arvense'nin farklı solventlerinden elde edilen ekstraktların antimikrobiyal ve antioksidan aktiviteleri araştırılmıştır. E. arvense ekstraktlarının antimikrobiyal aktivitesi disk difüzyon yöntemi kullanılarak tespit edilmiştir. Antimikrobiyal aktivite patojenik mikroorganizmalar Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, Candida albicans ve Candida glabrata kullanılarak belirlendi. Elde edilen sonuçlarda E. arvense’nin 500 µg konsantrasonda etanol ekstraktının farklı oranlarda (14,3-28,0) antimikrobiyal aktivite gösterdiği belirlendi. Etanol ekstraktı aynı konsantrasyonda Candida glabrata'ya (28,0 mm) karşı en yüksek antimikrobiyal aktivite göstermiştir. Kloroform ekstraktının kullanılan mikroorganizmalara karşı antimikrobiyal aktivite gösterdiği (7,3-10,6 mm) tespit edilmiştir. E. arvense'nin toprak üstü kısımlarının metanol ekstraktının farklı konsantrasyonlarındaki antioksidan aktivitesi, 2,2-diphenyl-1-picrilhydrazyl radikal temizleme kapasitesi yöntemine göre tespit edilmiştir. Metanol ekstraktının en yüksek radikal temizleme kapasitesi 10mg/mL (%91,5) konsantrasyonda görülmüştür. E. arvense’nin metanol ekstraktının IC50 değeri 3,13 mg/mL olarak hesaplandı.
Equisetum arvense L, also known as horsetail, is a medicinal plant used in traditional medicine. Especially, it is used in the treatment of bleeding, antiseptic, anti-inflammatory, urethritis, jaundice and hepatitis. In the study, the antimicrobial and antioxidant activities of extracts obtained from different solvents of E. arvense were investigated. Antimicrobial activity of E. arvense extracts was determined using the disc diffusion method. The antimicrobial activity was determined utilizing the pathogenic microorganisms Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, Candida albicans and Candida glabrata. In the results obtained, it was determined that the ethanol extract of E. arvense at 500 µg concentration showed antimicrobial activity at different rates (14.3-28.0). Ethanol extract showed the highest antimicrobial activity against Candida glabrata (28.0 mm) at the same concentration. It was detected that the chloroform extract showed antimicrobial activity (7.3-10.6 mm) against the microorganisms used. The antioxidant activity of the aerial parts of E. arvense at different concentrations of methanol extract was determined according to the 2,2-diphenyl-1-picrylhydrazil radical scavenging capacity method. The highest radical scavenging capacity of the methanol extract was observed at a concentration of 10mg/mL (91.5%). The IC50 value of the methanol extract of E. arvense was calculated as 3.13 mg/mL.
Equisetum arvense L, also known as horsetail, is a medicinal plant used in traditional medicine. Especially, it is used in the treatment of bleeding, antiseptic, anti-inflammatory, urethritis, jaundice and hepatitis. In the study, the antimicrobial and antioxidant activities of extracts obtained from different solvents of E. arvense were investigated. Antimicrobial activity of E. arvense extracts was determined using the disc diffusion method. The antimicrobial activity was determined utilizing the pathogenic microorganisms Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, Candida albicans and Candida glabrata. In the results obtained, it was determined that the ethanol extract of E. arvense at 500 µg concentration showed antimicrobial activity at different rates (14.3-28.0). Ethanol extract showed the highest antimicrobial activity against Candida glabrata (28.0 mm) at the same concentration. It was detected that the chloroform extract showed antimicrobial activity (7.3-10.6 mm) against the microorganisms used. The antioxidant activity of the aerial parts of E. arvense at different concentrations of methanol extract was determined according to the 2,2-diphenyl-1-picrylhydrazil radical scavenging capacity method. The highest radical scavenging capacity of the methanol extract was observed at a concentration of 10mg/mL (91.5%). The IC50 value of the methanol extract of E. arvense was calculated as 3.13 mg/mL.
Açıklama
Anahtar Kelimeler
Equisetum arvense, antimikrobiyal, antioksidan, tıbbi bitki., Equisteum arvense, antimicrobial, antioxidant, medicinal plant
Kaynak
Turkish Journal of Science and Technology
WoS Q Değeri
Scopus Q Değeri
Cilt
19
Sayı
2
