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    Effect of endosulfan on growth, ? amylase activity and plasmids amplification in Bacillus subtilis
    (Natl Inst Science Communication-Niscair, 2006) Tolan, V; Ensari, Y
    Endosulfan, a chlorinated hydrocarbon insecticide of cyclodiene subgroup acts as a contact poison in a wide variety of organisms. In the present study, the effect of endosulfan on the growth, alpha amylase activity and plasmid amplification was investigated in Bacillus subtilis system. The bacteria were grown in medium, incubated with different concentrations (32, 48, 64 and 80 mu g/mL) of endosulfan. The bacterial growth was gradually seen after 1st day at up to 48 mu g/l. endosulfan. The 48 mu g/L endosulfan inhibited approximately 50% of the bacterial growth. No growth was observed at and after 64 mu g/L endosulfan, for all days (1-5). Also, no a amylase activity was found in the supernatant of the Culture medium containing 64 and 80 mu g/L endosulfan, whereas slight activity was observed with 32 and 48 mu g/L endosulfan concentration. The amount of plasmid increased up to 50% in the presence of 32 mu g/L endosulfan. Endosulfan had no effect oil the alpha amylase activity in-vitro.
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    Öğe
    Effect of nonionizing radiation on plasmid DNA of E.coli puc9
    (Mbr Press Inc, 1999) Dasdag, S; Celik, MS; Uyar, F; Akdag, MZ; Sert, C; Ensari, Y
    The present experiment was carried out to investigate whether 9450 MHz and 2450 MHz microwave exposure (MW) affect to the plasmid DNA of Escherichia coli puc 9 or not. Suspensions of E.coli puc 9 were exposed to microwave radiation at 2450 MHz (55,110,165,220 and 275 W for 30 and 60 second) and 9450 MHz (2.65mW/cm(2) for 10,20,40 and 60 minutes). However, plasmid DNA of E.coli puc 9 were also exposed to 9450 MHz (10,20,40 and 60 minutes) and 2450 MHz (55,110,165,220 and 275 W for 30 and 60 second) Microwave. After exposures of E.coli puc 9, the plasmid DNA were isolated and evaluated by AGE assay. We found that amount of plasmid DNA was not changed in 9450 MHz and 2450 MHz MW exposure. The difference of temperatures between before and after 2450 MHz MW exposures were found to be significant (P<0.01). It was concluded that E.coli puc 9 plasmid DNA was not affected by 9450 MHz and 2450 MHz Microwave radiation.