Effect of endosulfan on growth, ? amylase activity and plasmids amplification in Bacillus subtilis
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Tarih
2006
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Natl Inst Science Communication-Niscair
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Endosulfan, a chlorinated hydrocarbon insecticide of cyclodiene subgroup acts as a contact poison in a wide variety of organisms. In the present study, the effect of endosulfan on the growth, alpha amylase activity and plasmid amplification was investigated in Bacillus subtilis system. The bacteria were grown in medium, incubated with different concentrations (32, 48, 64 and 80 mu g/mL) of endosulfan. The bacterial growth was gradually seen after 1st day at up to 48 mu g/l. endosulfan. The 48 mu g/L endosulfan inhibited approximately 50% of the bacterial growth. No growth was observed at and after 64 mu g/L endosulfan, for all days (1-5). Also, no a amylase activity was found in the supernatant of the Culture medium containing 64 and 80 mu g/L endosulfan, whereas slight activity was observed with 32 and 48 mu g/L endosulfan concentration. The amount of plasmid increased up to 50% in the presence of 32 mu g/L endosulfan. Endosulfan had no effect oil the alpha amylase activity in-vitro.
Açıklama
Anahtar Kelimeler
Endosulfan, Bacillus Subtilis, Alpha Amylase, Plasmid
Kaynak
Indian Journal of Biochemistry & Biophysics
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
43
Sayı
2
