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    ?-AMYLASE FROM BACILLUS SIMPLEX- PRODUCTION, CHARACTERIZATION AND PARTIAL PURIFICATION
    (Parlar Scientific Publications (P S P), 2017) Ortakaya, Veysi; Fincan, Sema Aguloglu; Enez, Baris
    In this study, some bacteria were isolated from the soil obtained from Ergani Makam Mountain. The isolated bacteria were defined as Bacillus simplex with the biochemical tests and 16S rRNA analysis. The optimum conditions for bacterial growth were determined as the 32th hour, 37 degrees C and pH 7.0. While the maximum amylase activity was observed of the 72nd hour, the optimum temperature and pH of the enzyme were determined to be 37 degrees C and 7.0 reciprocally. It was observed that the enzyme production increased with ammonium sulfate and ammonium nitrate from nitrogen sources and decreased with the addition of carbon sources. Amylase was partially purified with 70% ammonium sulfate precipitations and dialysis. When the effect of detergents was examined on the partially purified enzyme it was determined that SDS considerably inhibited and the other detergents increased the enzyme activity. It was also determined that while CaCl2 increased the activity whereas FeCl2, CuCl2, ZnCl2 and HgCl2 considerably inhibited the enzyme activity.
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    Comparison of Methods for the Preconcentration of Cadmium (II) Using Amberlite XAD-16 Resin Modified with Anoxybacillus caldiproteolyticus and Geobacillus stearothermophilus as Novel Biosorbents
    (Taylor & Francis Inc, 2020) Enez, Baris; Oral, Elif Varhan; Fincan, Sema Aguloglu; Ziyadanogullari, Berrin
    The isolation, identification and characterization of bacteria obtained from soil of Ergani Makam Mountain were performed and the results revealed that the bacteria were thermophilic Anoxybacillus caldiproteolyticus. The characterized bacteria and purchased Geobacillus stearothermophilus were immobilized on Amberlite XAD-16 in order to prepare two biosorbents for preconcentration experiments for the determination of cadmium (II) ions. The produced biosorbents were enriched separately using mini-columns and the analyte was determined by flame atomic absorption spectrometry. The effects of solution parameters were investigated for the separation and preconcentration yields. The recovery efficiencies of Amberlite XAD-16 immobilized separately with A. caldiproteolyticus and G. stearothermophilus were determined to be 98.23 +/- 2.40 and 98.93 +/- 1.3 (n = 5) for the optimum working conditions, respectively. Moreover, the Cd (II) ion was recovered with 10 mL of 1 mol L-1 of HCl and 2 mL of 0.5 mol L-1 HNO3 solutions. The optimum working conditions were determined to be at pH 6.0 and a flow rate of 2 mL min(-1) for both biosorbents. The recovery efficiencies of matrix ions were characterized to investigate the feasibility of the developed preconcentration methods. The accuracy of the proposed methods were controlled by analyzing a SCP Science EnviroMAT Waste Water, Low (EU-L-2) certified reference material. The obtained results were comparable to the certified values. These methods were also applied to the analysis of water samples from Dicle River, Hazar Lake and Diyarbakir tap water for Cd (II).
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    Isolation and characterization of ?-amylase from thermophilic Geobacillus stearothermophilus
    (Elsevier Sci Ltd, 2011) Enez, Baris; Fincan, Sema Aguloglu
    [Abstract Not Available]
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    ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF THERMOSTABLE XYLANASE FROM THERMOPHILIC ANOXYBACILLUS SP ISOLATED FROM HOT SPRINGS
    (Parlar Scientific Publications (P S P), 2016) Enez, Baris; Fincan, Sema Aguloglu
    Isolation, partial purification and characterization of thermostable xylanase from thermophilic Anoxybacillus isolated from hot springs located in Afyonkarahisar were performed. The bacterium was determined to be bacilli (rods) shape, gram-positive and movable. Optimum conditions for bacterial growth and enzyme production were determined to be 55 degrees C, pH 7.0 and 48 hours. Maximum anzyme activity was reached at 60 degrees C and pH 7.0. Partial purification of xylanase enzyme was performed with precipitation of 80% ammonium sulfate and dialysis procedures (1.2-fold and 35% recovery, respectively). The molecular weight of xylanase was similar to 34 kDa, as estimated by SDS-PAGE. Partially purified anzyme was observed to maintain its stability after 2 hours in 50 degrees C and 60 degrees C and after 1 hour in 70 degrees C temperature. It was determined that enzyme activity was maintained 100% at the end of the 1-hour period at pH 6.0, 7.0 and 8.0. A particularly strong inhibition was observed with the SDS (83%) effect caused by detergents used on enzyme inhibition.
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    Preparation and characterization of a novel magnetic biosorbent functionalized with biomass of Bacillus Subtilis: Kinetic and isotherm studies of biosorption processes in the removal of Methylene Blue
    (Elsevier Sci Ltd, 2017) Tural, Bilsen; Ertas, Erdal; Enez, Baris; Fincan, Sema Aguloglu; Tural, Servet
    A novel magnetic biosorbent (M-BSub) was successfully prepared by the immobilization of Bacillus subtilis (B. subtilis) with nano-sized magnetic silica which was confirmed by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), vibrating sample magnetometry (VSM), scanning electron microscope (SEM). Optimum biosorption conditions were determined as a function of pH, contact time and initial concentration of Methylene Blue (MB). The maximum biosorption efficiency of MB was obtained at pH 6.8 and 30 degrees C. The uptake of dye was very fast initially, and achieved equilibrium after 3 h. The isotherm models like Langmuir, Freundlich, Dubinin-Radushkevich (D-R) were used to analyze the equilibrium data. The equilibrium data fit better to the Freundlich model compared to the Langmuir model in concentration range studied (50-300 mg/L). The maximum biosorption capacity of biosorbent reached up to 59 +/- 0.6 mg g(-1) at pH value of 6.8 and 30 degrees C. The kinetics data were analyzed using adsorption kinetic models like the pseudo-first order, pseudo-second order and intraparticle diffusion equation. Kinetic data showed good agreement with the pseudo-second order kinetic model. In addition, biosorbent can be easily regenerated and reused for five cycles with high biosorption capacity. This study indicated that M-BSub is a reusable biosorbent for rapid, convenient, and efficient removal of MB from contaminated aquatic systems.
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    Purification and characterization of a thermostable ?-amylase from Geobacillus stearothermophilus
    (Elsevier Sci Ltd, 2011) Fincan, Sema Aguloglu; Enez, Baris; Rezzukoglu, Ihsan
    [Abstract Not Available]
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    Purification and characterization of thermostable a-amylase produced from Bacillus licheniformis So-B3 and its potential in hydrolyzing raw starch
    (Pergamon-Elsevier Science Ltd, 2021) Fincan, Sema Aguloglu; Ozdemir, Sadin; Karakaya, Adem; Enez, Baris; Mustafov, Sibel Demiroglu; Ulutas, Mehmet Sefa; Sen, Fatih
    Aims: This work was achieved to obtain the optimum culture conditions of the thermostable alpha-amylase produced by thermophilic Bacillus licheniformis SO-B3. Furthermore, the alpha-amylase was purified and then characterized, and also its kinetic parameters were determined. Materials and methods: A new thermotolerant bacteria called Bacillus licheniformis SO-B3 employed in this work was isolated from a sample of thermal spring mud in Sirnak (Meyremderesi). Several parameters such as the impact of temperature, time, and pH on enzyme production were examined. Thin-Layer Chromatography (TLC) was employed to analyze the end-products of soluble starch hydrolysis, and the utilization of purified alpha-amylase in the clarification of unripe apple juices was studied. Key findings: The highest enzyme production conditions were determined as 35 degrees C, 36th hour, and pH 7.0. Thermostable alpha-amylase was purified by 70% ammonium sulfate precipitation, DEAE-cellulose ion-exchange chromatography, and dialysis, with a 51-purification fold and 30% yield recovery. The K-m and V-max values for this enzyme were 0.004 mM and 3.07 mu mol min(-1) at 70 degrees C, respectively. The alpha-amylase's molecular weight was found as 74 kDa. In addition, alpha-amylase showed a good degradation rate for raw starch. Significance: It was hypothesized that Bacillus licheniformis SO-B3 could be used as an alpha-amylase source. These findings displayed that purified enzyme could be utilized in fruit juice industries for clarification of apple juice and raw starch hydrolyzing.