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    Phenolic content and biomolecule oxidation protective activity of Globularia alypum extracts
    (Instituto de Tecnologia do Parana, 2017) Bouriche, Hamama; Kada, Seoussen; Senator, Abderrahmane; Demirtaş, İbrahim; Özen, Tevfik; Toptancı, Bircan Çeken; Kızıl, Göksel; Kızıl, Murat
    The protective activity of methanolic (Met E) and aqueous (Aq E) extracts of Globularia alypum L. (G. alypum) against DNA, lipid and protein oxidative damage was investigated. Moreover, the scavenging, chelating, and reducing power activities of the extracts were also evaluated. Phytochemical analysis was performed to determine phenolic compounds. Results showed that Met E and Aq E were rich in phenolic compounds, and were able to scavenge DPPH* with IC50 values of 48.61 μg/mL and 51.97 μg/mL, respectively. In addition, both extracts were able to chelate ferrous ions. At 300 μg/mL, the chelating activity was 97.53% and 91.02%, respectively. The reducing power of these extracts was also remarkable and concentration dependent. At 100 μg/mL, both extracts inhibited lipid peroxidatin by only 42.45% and 4.03%. However, the DNA oxidation damage was inhibited dose-dependently in the presence of G. alypum extracts. At 1 mg/mL, both extracts suppressed DNA cleavage by 83%-84%. The protein oxidation was also inhibited by G. alypum extracts. At 1 mg/mL, Aq E and Met E protected BSA fragmentation by 77%-99%. The overall results suggest that G. alypum extracts exerted antioxidant activity and protect biomolecules against oxidative damage; hence it may serve as a potential source of natural antioxidants.
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    Phytochemical analysis and biological activity of Corchorus olitorius L.: Quantitative analysis of bioactive compounds by LC–MS/MS, antibacterial, enzyme inhibition, and cytotoxic activities
    (Elsevier GmbH, 2023) Atalar, Mehmet Nuri; Erenler, Ramazan; Turkan, Fikret; Alma, Mehmet Hakkı; Demirtaş, İbrahim; Baran, Ayşe; Kandemir, Sevgi İrtegün
    Introduction: Corchorus olitorius L. is a significant plant in folk medicine, therefore, research on phytochemistry with biological activity may reveal its potential use in drug development. Medicinal plants are valuable sources of drug materials as they include bioactive compounds known as secondary metabolites. Determination of bioactive compounds and biological activity of plants lead to understanding their potential in drug development. Methods: A quantitative analysis of the bioactive compounds in leaf, stem, and root extracts of C. olitorius L. was carried out with Liquid Chromatography-Mass Spectrometry (LC–MS/MS). Acetylcholinesterase (AChE) and Glutathione-S-Transferase (GST) enzyme inhibition and antibacterial effects were investigated using Mueller Hinton broth microdilution assay. The anticancer activity of C. olitorius leaves was evaluated by MTT assay using HDF, U87, Skov-3, and Caco-2 cell lines. Results: In the quantitative study, leaf extract exhibited the largest concentration of bioactive compounds, and cynarin (6.680 mg/g extract) and chlorogenic acid (5.605 mg/g extract) were the major products. The leaf, stem, and root extracts showed significant AChE and GST inhibition activity. The leaf extract displayed antibacterial activity against P. aeruginosa with the value of 2.2±0.1 (IC50, μg/ mL) in comparison to the standard (4.00±0.1 IC50, μg/ mL). The leaf extract displayed good antiproliferative activity against sk-ov-3 cell lines with values of 37.1% and 42.1% at 250 μg/mL and 500 μg/mL concentrations, respectively. Conclusion: C. olitorius has the potential to be a drug agent against cancer and microbial-induced diseases due to its effective bioactive compound contents.