Phenolic content and biomolecule oxidation protective activity of Globularia alypum extracts
Yükleniyor...
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Instituto de Tecnologia do Parana
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The protective activity of methanolic (Met E) and aqueous (Aq E) extracts of Globularia alypum L. (G. alypum) against DNA, lipid and protein oxidative damage was investigated. Moreover, the scavenging, chelating, and reducing power activities of the extracts were also evaluated. Phytochemical analysis was performed to determine phenolic compounds. Results showed that Met E and Aq E were rich in phenolic compounds, and were able to scavenge DPPH* with IC50 values of 48.61 μg/mL and 51.97 μg/mL, respectively. In addition, both extracts were able to chelate ferrous ions. At 300 μg/mL, the chelating activity was 97.53% and 91.02%, respectively. The reducing power of these extracts was also remarkable and concentration dependent. At 100 μg/mL, both extracts inhibited lipid peroxidatin by only 42.45% and 4.03%. However, the DNA oxidation damage was inhibited dose-dependently in the presence of G. alypum extracts. At 1 mg/mL, both extracts suppressed DNA cleavage by 83%-84%. The protein oxidation was also inhibited by G. alypum extracts. At 1 mg/mL, Aq E and Met E protected BSA fragmentation by 77%-99%. The overall results suggest that G. alypum extracts exerted antioxidant activity and protect biomolecules against oxidative damage; hence it may serve as a potential source of natural antioxidants.
Açıklama
Anahtar Kelimeler
Antioxidants, DNA damage, Globularia alypum, Lipid peroxidation, Protein oxidation
Kaynak
Brazilian Archives of Biology and Technology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
60
Sayı
Künye
Bouriche, H., Kada, S., Senator, A., Demirtaş, İ., Özen, T., Toptancı, B. Ç. ve diğerleri. (2017). Phenolic content and biomolecule oxidation protective activity of Globularia alypum extracts. Brazilian Archives of Biology and Technology, 60, 1-15.
