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Öğe The effect of phenolic amino acids on differentiation of DNA, RNA, proteins and cell growth in Cicer arietinum L.(Mbr Press Inc, 1999) Otludil, B; Akbayin, H; Taskin, T; Demir, RThe effect of three amino acids (phenilalanine tyrosine triptophane) have been examined on differentiation of DNA, RNA, protein synthesis and cell growth in cell culture for Cicer arietinum L. cultivated in Murashige and Skoog medium. The Cicer arietinum L, culture were treated with 20, 40,60,80 and 100 mu g. levels of each phenolic amino acid. Amount of DNA, RNA, proteins and cell growth were determined by the levels in the time intervals of 24 h incubation time during 6 days. Obtained data were evaluated statistically Consequently, experimental results reveal that excessive amount of phenolic amino acids cause significant inhibition on RNA, protein and cell growth. Phenilalanine not strongly effect on DNA synthesis or activity of DNA polymerase was unaffected by phenilalanine but tyrosine and triptophane has significant effect on DNA. On the other hand, it has been supposed that extreme amount of phenolic amino acids may be converted to phenolic compounds by cell regulatory systems.Öğe The formation of callus cultures from unpollinated pistils in cotton plant (Gossypium hirsutum L)(Mbr Press Inc, 1998) Taskin, T; Demir, R; Otludil, B; Colak, GIn this study, unpollinated floral buds of cotton (Gossypium hirsutum L.) were cultured on the Murashige and Skoog (MS) media containing Kinetin (Kin) and Indol Asetic Acid (IAA) at different concentrations for the callus cultures, The nutrient medium containing 2 mg/l Kin + 1 mg/l IAA was observed to be the best medium for the callus formation from ovule and ovarium tissues.Öğe Investigation of the saturated and unsaturated fatty acids in the internodes and leaves of Vitis vinifera L. (Mazruma)(Mbr Press Inc, 1997) Demir, R; Otludil, BIn this study, the amounts of saturated (palmitic acid, stearic acid) and unsaturated (oleic acid, linoleic acid, linolenic acid) fatty acids in the samples belonging to internodes 1-2-3, 5-6 and 9-10, and of saturated (palmitic acid, stearic acid) and unsaturated (oleic acid, linoleic acid, linolenic acid) fatty acids in the leaf samples belonging to internodes 1-2-3, 5-6 and 9-10 of Vitis vinifera L.(Mazruma) were investigated, depending on the vegetation period. According to ripening period, great variations were seen in the total fatty acids amounts. Unsaturated fatty acids were found to be higher with respect to saturated fatty acids. However, any significant differences were not found between saturated and unsaturated fatty acids in the specimens belonging to all internodes and in the leaf samples belonging to these internodes.Öğe A study on the investigation of fatty acids in pulps and seeds of Olea europaea L. (Hirhali) and Olea eoropaea L. (Zoncuk).(Mbr Press Inc, 1998) Demir, R; Bashan, MThe composition of fatty acids in pulps and seeds of Olea europaea L. (Hirhali) and Olea europaea L. (Zoncuk) was investigated. Palmitic, palmitoleic stearic, oleic, linoleic and linolenic fatty acids were determined in phospholipid and neutral lipid fractions of pulps. In both fractions, oleic acid was seen as the highest, while palmitoleic and linolenic acids were at the lowest levels. In phospholipid fraction of Zoncuk, palmitoleic, and in neutral lipid fraction, linolenic acid were not determined in the seeds, oleic acid was found as the highest. In addition, myristic acid, which was not determined in phospholipid and neutral lipid fractions, was found it the seeds.
