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Öğe Comparison of Cytotoxicity of Four Different Adhesive Materials Before and After Polymerisation(Quintessence Publishing Co Inc, 2020) Cangul, Suzan; Adiguzel, Ozkan; Tekin, SametIntroduction: The aim of this study was to make a quantitative comparison of the cytotoxic potentials of four different polymerised and unpolymerised self-etching adhesives which were newly used clinically. Materials and Methods: Cytotoxic effects of both polymerised and unpolymerised forms of all test adhesives were evaluated against L929 cell line using the MTT test. The activity for unpolymerised adhesives was assessed in different doses and incubation times manner. On the other hand, cytotoxicity of the polymerised adhesives prepared at different extraction times were evaluated as dependent on incubation times. Two-Way Variance Analysis and Bonferroni post-test was used for statistical evaluation. Results: There were statistically significant differences between the groups (p < 0.05). In general, it was shown that unpolymerised and polymerised forms of each of the test compounds exhibited a time-dependent cytotoxic effect. However, the effect on polymerised forms was found to be independent of the duration of the extraction, while the effect on the unpolymerised forms increased dose-dependently. It was also determined that the most cytotoxic material in the unpolymerised form was Dentsply and in the polymerised form was Tokuyama. Conclusions: Dentsply should be preferred over Tokuyama to be able to provide clinically long-lasting restorations.Öğe Do ozone and boric acid affect microleakage in class V composite restorations?(Taylor & Francis Inc, 2019) Cangul, Suzan; Yildirim, Zehra Susgun; Bahsi, Emrullah; Sagmak, Savas; Satici, OmerThe aim of this in vitro study was to evaluate the effects of chlorhexidine gluconate (2%), sodium hypochloride (2.5%), ozone gas, and boric acid at different concentrations (1%, 3%, 5%, and 7%) on microleakage from composite restorations.In a total of 80 extracted human canine teeth, a class V cavity was opened on the buccal surface and the samples were separated into eight groups. In the control group, no procedure was applied for cavity disinfection, then composite restoration (Z250, 3M) was made using single-stage, self-etch adhesive (Single Bond 3M). In the other groups, seven different disinfectants were used, then the cavity was restored. The teeth were split into two in the buccolingual direction, parallel to the long axes. Stain penetration was examined under stereomicroscope and scored. Examination with SEM was made on one sample from each group, selected at random. Statistical evaluations were made using Dunnett C Post Hoc Comparison and Kruskal-Wallis H tests.In the occlusal region evaluation, the groups with the lowest level of leakage were the 3% and 5% boric acid groups, and the highest levels of microleakage were determined in the chlorhexidine group and the 1% boric acid group. In the gingival region, the lowest level of microleakage was in the 5% boric acid group and the highest levels were determined in the 1% and 7% boric acid groups.Boric acid disinfectants used at suitable concentrations were not seen to create a risk in respect of microleakage.Öğe Does the Use of Ozone as a Cavity Disinfectant Affect the Bonding Strength of Antibacterial Bonding Agents?(Taylor & Francis Inc, 2020) Cangul, Suzan; Erpacal, Begum; Adiguzel, Ozkan; Sagmak, Savas; Unal, Server; Tekin, SametThe aim of this study was to evaluate the use of ozone as cavity disinfectant on the bonding strength to dentin of different antibacterial bonding agents. The study was conducted on 60 cavity-free 3rd molar teeth. The prepared samples were separated into 3 groups. These groups were each sub-divided into 2 groups of 10 as a group with ozone applied and a control group. In Group 1a, ozone was applied as cavity disinfectant for 6 secs to all the exposed dentin surfaces then restoration was applied. In Group 1b as the control group, the restoration stages were applied without any cavity disinfection. Gluma 2 Bond, our first antibacterial adhesive material, was applied to all dentin surfaces and polymerized. The second and third groups were divided into two groups. Clearfil SE Protect Primer and Clearfil SE Protect Bond were applied in the group 2. Peak Universal was used in Group 3. After the application of the adhesive materials and then Estelite posterior quick composite was applied and polymerized. The bonding strengths of all the samples were measured in an Instron test device. The data were statistically analyzed using the Mann Whitney U-test and the Kruskal Wallis test. The highest bonding strength was determined in Gluma 2 Bond (7,79), and the lowest bonding strength was in Peek Universal (3,43). It was concluded that ozone adhesive systems can be safely currently used as they have been shown to increase bonding strength and can eliminate bacteria.Öğe Evaluation of Microleakage Caused by Different LED Light Sources in Composite Resins with AutoCAD Program(Sci Printers & Publ Inc, 2020) Cangul, Suzan; Atas, Osman; Adiguzel, Ozkan; Erpacal, Begum; Bakir, Metin; Eskibaglar, MehmetOBJECTIVE: To use AutoCAD software to examine the microleakage of bulk-fill composites occurring as a result of polymerization with different light sources. STUDY DESIGN: In a total of 40 extracted first molar teeth, class I cavities were opened. Then 4 groups were formed of 10 samples in each group. All the samples were restored with SDR bulk-fill composite. The samples were polymerized with an LED light source as follows: Group 1, Mikado; Group 2, Woodpecker; Group 3, Planmeca; and Group 4, CarboLED. Each tooth was divided into 2 sections. Each section was examined under stereomicroscope, and the dimensions of the leakage surface area were measured with AutoCAD software. In the statistical evaluations of the data, the Kruskal-Wallis and Tukey B tests were used. RESULTS: Significant differences were found between the groups in the statistical evaluations (p <0.05). The highest value was found in the CarboLED group and the lowest value was found in the group where Mikado light source was used. CONCLUSION: There are many factors affecting polymerization. Since there are insufficient studies on this subject, it should be supported with new research.Öğe Evaluation of the use of PEEK material in post-core and crown restorations using finite element analysis(Mosher & Linder, Inc, 2020) Tekin, Samet; Adiguzel, Ozkan; Cangul, Suzan; Atas, Osman; Erpacal, BegumPurpose: To compare the stresses generated after the use of low elastic modulus polyetheretherketone (PEEK) as dental post and crown material with glass fiber post and metal supported ceramic crowns using finite element analysis. Methods: First an image of a maxillary central incisor taken on micro-computed tomography (microCT) was uploaded to a computer program. Modeling was made of all the materials in the computer environment and 3-dimensional (3D) virtual models were obtained. Using two different post materials (glass fiber and PEEK post) and two different crown materials (metal supported ceramic and PEEK crown), the modeling of the maxillary central tooth was completed and a total of four groups were obtained. Finally, 135 degrees and 100 N forces were applied from the midpoint of the palatal surface. Results: The FEA analysis showed the use of PEEK as post material decreased the stress values in the post, post cement and composite core models and did not have any significant change in the dentin and crown cement models. The use of composite veneer with PEEK substructure as crown material reduced stress values in dentin, the post and composite core and increased stress values in both the post cement and the crown cement.