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    Phenolic content and biomolecule oxidation protective activity of Globularia alypum extracts
    (Instituto de Tecnologia do Parana, 2017) Bouriche, Hamama; Kada, Seoussen; Senator, Abderrahmane; Demirtaş, İbrahim; Özen, Tevfik; Toptancı, Bircan Çeken; Kızıl, Göksel; Kızıl, Murat
    The protective activity of methanolic (Met E) and aqueous (Aq E) extracts of Globularia alypum L. (G. alypum) against DNA, lipid and protein oxidative damage was investigated. Moreover, the scavenging, chelating, and reducing power activities of the extracts were also evaluated. Phytochemical analysis was performed to determine phenolic compounds. Results showed that Met E and Aq E were rich in phenolic compounds, and were able to scavenge DPPH* with IC50 values of 48.61 μg/mL and 51.97 μg/mL, respectively. In addition, both extracts were able to chelate ferrous ions. At 300 μg/mL, the chelating activity was 97.53% and 91.02%, respectively. The reducing power of these extracts was also remarkable and concentration dependent. At 100 μg/mL, both extracts inhibited lipid peroxidatin by only 42.45% and 4.03%. However, the DNA oxidation damage was inhibited dose-dependently in the presence of G. alypum extracts. At 1 mg/mL, both extracts suppressed DNA cleavage by 83%-84%. The protein oxidation was also inhibited by G. alypum extracts. At 1 mg/mL, Aq E and Met E protected BSA fragmentation by 77%-99%. The overall results suggest that G. alypum extracts exerted antioxidant activity and protect biomolecules against oxidative damage; hence it may serve as a potential source of natural antioxidants.
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    Phytochemical analysis, and antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex L. and Pinus halepensis Mill. methanolic extracts
    (Journal Pharmacy & Pharmacognosy Research-Jppres, 2019) Meziti, Hicham; Bouriche, Hamama; Kada, Seoussen; Demirtas, Ibrahim; Kizil, Murat; Senator, Abderrahmane
    Context: Quercus ilex and Pinus halepensis are largely used in Algerian folk medicine as a remedy for different health problems. Aims: To determine the phytochemical composition as well as to evaluate the antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex root bark extract (QIE) and Pinus halepensis young ovulate cones extract (PHE). Methods: Methanolic extracts were prepared by maceration. Phenolic compounds and flavonoids were quantified spectrophotometrically and identified by HPLC analysis. In vitro antioxidant activity of the extracts was assessed by determining the DPPH and ABTS radicals scavenging activities and ferric reducing/antioxidant power (FRAP). Protective effect of the extracts against AAPH-induced erythrocyte hemolysis was assessed. pBluescript M13 (+) plasmid DNA was used as oxidation target to evaluate DNA protective effect of the extracts. Results: Considerable phenolic compounds and flavonoids contents were found in the studied extracts. Catechin and phenolic acids (4-hydoxybenzoic, caffeic, coumaric, ferulic and gentisic acids) were identified in QIE, while catechin and cinnamic acid were identified in PHE. Both extracts scavenge DPPH radical with IC50 values of 5.67 mu g/mL and 18.87 mu g/mL, respectively. Antioxidant capacity against ABTS radical was 4.13 and 1.15 mM Trolox equivalent/mg extract. The two extracts also showed a considerable ferric reducing ability with 1.98 and 8.55 mM FeSO4/mg extract, respectively. On the other hand, both extracts exerted a significant protective effect against AAPH-induced erythrocyte hemolysis and DNA protective activities were noticed. Conclusions: Quercus ilex and Pinus halepensis extracts are important sources of bioactive compounds possessing important antioxidants, antihemolytic and genoprotective effects.
  • [ X ]
    Öğe
    Protective activity of Hertia cheirifolia extracts against DNA damage, lipid peroxidation and protein oxidation
    (Taylor & Francis Ltd, 2017) Kada, Seoussen; Bouriche, Hamama; Senator, Abderrahmane; Demirtas, Ibrahim; Ozen, Tevfik; Toptanci, Bircan Ceken; Kizil, Goksel
    Context: Hertia cheirifolia L. (Asteraceae), a perennial shrub widely distributed in Northern Africa, is traditionally used to treat inflammatory disorders. Objective: The protective effect of methanol (Met E) and aqueous (Aq E) extracts of Hertia cheirifolia against DNA, lipid and protein oxidation was investigated. Materials and methods: Different concentrations (50-1000 mu g/mL) of Hertia cheirifolia aerial part extracts were examined against DNA, lipid and protein oxidation induced by H2O2 +UV, FeSO4, and Fe3+/H2O2-ascorbic acid, respectively. The DPPH center dot, metal ion chelating, reducing power and beta-carotene bleaching tests were conducted. Results: Both extracts were rich in polyphenols, flavonoids and tannins, and were able to scavenge DPPH center dot with IC50 values of 138 and 197 mu g/mL, respectively. At 300 mu g/mL, Aq E exerted stronger chelating effect (99%) than Met E (69%). However, Met E reducing power (IC50 = 61 mu g/mL) was more than that of Aq E (IC50 = 193 mu g/mL). Both extracts protected from beta-carotene bleaching by 74% and 94%, respectively, and inhibited linoleic acid peroxidation. The inhibitory activity of Aq E extract (64%) was twice more than that of Met E (32%). Interestingly, both extracts protected DNA against the cleavage by about 96-98%. At 1 mg/mL, Met E and Aq E restored protein band intensity by 94-99%. Conclusions: Hertia cheirifolia exhibits potent antioxidant activity and protects biomolecules against oxidative damage; hence, it may serve as potential source of natural antioxidant for pharmaceutical applications and food preservation. This is the first report on the protective activity of this plant against biomolecule oxidation.