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Yazar "Bouhafsoun, Aicha" seçeneğine göre listele

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    Determination of anticholinesterase and antioxidant activities of methanol and water extracts of leaves and fruits of Chamaerops humilis L.
    (Applied and Natural Science Foundation, 2019) Bouhafsoun, Aicha; Boğa, Mehmet; Boukeloua, Ahmed; Temel, Hamdi; Kaid-Harche, Meriem
    The present study was carried out to investigate the antioxidant activity of the water and methanol extracts of leaves and fruits extracts of Chamaerops humilis L. by using ABTS cation radicals and cupric reducing antioxidant capacity (CUPRAC). Anticholinesterase effect of the extracts was tested against both AChE and BChE using a microplate-reader assay based on the Ellman method. The methanol extracts of C. humilis leaves contained relatively higher content of flavonoids and total phenolics than those of fruits. All the extracts were found to have different levels of antioxidant activity in the systems tested. The leave extract showed the highest value of antioxidant activity, based on ABTS radical-scavenging activity, while the fruit water extract showed the highest value (0.53±0.50 µg/ mL) of cupric reducing antioxidant activity. Our data indicates that both methanol and water fruit extract were active for BChE inhibition (31.65 ± 0.37 and 30.19 ± 0.56%) respectively, whereas, all leave extracts did not show any activity against BChE. The present study demonstrated that the methanol and water extracts fractions of C. humilis have different responses with different antioxidant methods. Our results suggest that the C. humilis could be used as a source of antioxidant agents and may be beneficial in the AD treatment.
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    Simultaneous quantification of phenolic acids and flavonoids in Chamaerops humilis L. using LC-ESI-MS/MS
    (Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA, 2018) Bouhafsoun, Aicha; Yılmaz, Mustafa Abdullah; Boukeloua, Ahmed; Temel, Hamdi; Kaid, Meriem Harche
    In this study, heated reflux extraction method has been used to identify the phenolic compounds from C. humilis var. argentea leaflets, rachis and fruits. Extractions were performed in both ultrapure water and 80% methanol solvents. The efficiency of procedures was determined in terms of the quality and quantity of phenolic acids and flavonoids identified. Chamaerops extracts have been characterized by high concentrations of phenolic compounds, which play a crucial role in protection against various diseases. LC-MS/MS was used to determine the chemical profile of various extracts obtained from Chamaerops. The results showed that the major components in leaflets and fruits extracts were quinic, malic and chlorogenic acids. In addition, nine minor acidic components were identified. On the other hand, rutin and hesperidin were found to be the major flavonoids. The methanol extract was shown as being the most efficient to identify phenolic compounds in C. humilis.

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