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    Öğe
    Comparison of total lipid and fatty acid compositions of whole-body and body segments of lertha extensa adults (Neuroptera: Nemopteridae)
    (2009) Cakmak O.; Bashan M.; Satar A.
    Total lipid and fatty acid compositions of phospholipids and triacylglycerols fractions, prepared from whole body and selected body segments (head, thorax and abdomen) of adults males and females of Lertha externa Oliver (Neuroptera: Nemopteridae), were analyzed by gas chromatography and gas chromatography-mass spectrometry. The female abdomen has the highest level of total lipid in body segments. Predominant fatty acid components of phospholipid and triacylglycerol fractions in the whole body and three body segments were Cl6:0, C18:ln-9 and C18:2n-6 acids, comprising more than 80 % of the fatty acid components. Fatty acid profiles of whole body extracts differed by sex only for phospholipid and triacylglycerol fractions. Fatty acid profiles of phospholipids from the head and abdomen were similar to those of the whole body, but considerably different from that of thorax. Several minor fatty acids, comprising < 2% of the total, were noted. Detailed profiles are reported, and are compared to those of other insects.
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    Öğe
    The effects of ionic and non-ionic detergents on Escerichia coli ?-galactosidase activity
    (1998) Guven K.; Bashan M.
    The effects of several ionic and non-ionic detergents on ?-Galactosidase activity at 30°C have been evaluated. The detergent concentrations tested were in the ranges that are already used in most assays to permeablise and reveal proteins from most tissues. The results obtained indicate that the anionic detergent SDS at all concentrations inhibited ?-Galactosidase activity, whereas non-ionic detergents, Triton-X 100 and Tween 20 have no effects on the enzyme activity. The electrophoretic analysis of SDS-exposed enzyme shows two bands corresponding to 170 kD and 116 kD, only the former being active.
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    Öğe
    A study on the investigation of fatty acids in pulps and seeds of Olea europaea L. (Hirhali) and Olea europaea L. (Zoncuk)
    (1998) Demir R.; Bashan M.
    The composition of fatty acids in pulps and seeds of Olea europaea L. (Hirhali) and Olea europaea L. (Zoncuk) was investigated. Palmitic, palmitoleic stearic, oleic, linoleic and linolenic fatty acids were determined in phospholipid and neutral lipid fractions of pulps. In both fractions, oleic acid was seen as the highest, while palmitoleic and linolenic acids were at the lowest levels. In phospholipid fraction of Zoncuk, palmitoleic, and in neutral lipid fraction, linolenic acid were not determined in the seeds, oleic acid was found as the highest. In addition, myristic acid, which was not determined in phospholipid and neutral lipid fractions, was found it the seeds.