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Öğe Analysis of selected marker studies on minor poaceae forages(Hasan Önder, 2023) Bayhan, Bora; Baran, NurettinPastures are very important for agriculture since the main feeding source of the enormous cattle herd in the world is forage. Pastures comprise plants from several genera of grasses and legumes. In terms of the physical variety, ecology, and economic significance, the Poaceae is among the most significant families of Angiosperms. Regarding stress tolerance, species within this family exhibit a very wide range of variation. In recent years, the importance of using molecular markers in phylogenetic analyses of numerous organisms has increased. The development of genomic technologies and infrastructure has progressed sufficiently for their use in marker- aided selection (MAS) to be studied in several important perennial fodder species. Differences can be directly attributed to minor variations in the genetic code such as phenotype, single sequence repeats (SSRs), and single nucleotide polymorphisms (SNPs). Therefore, breeders can benefit significantly from developing and characterizing new genetic markers. This paper gives a brief analysis of some international studies on some minor Poacea forages.Öğe Bazı pamuk (Gossypium hirsutum L.) çeşitlerinin çimlenme ve erken fide gelişimi dönemlerinde tuz stresine olan tepkileri(Türk Ziraat Yüksek Mühendisleri Birliği, 2023) Çiçek, Şilan; Yalınkılıç, Nazlı Aybar; Başbağ, Sema; Baran, NurettinSalt stress is one of the most important abiotic stress factors that negatively affect the growth and development of plants and cause yield losses. Plants are more sensitive to stress factors during germination and early seedling development. It is more appropriate to determine the negative effects of salinity on plants during these periods. This study was carried out to examine the effects of different salt doses on germination and early development of some cotton seeds. The study was carried out in randomized plots in a factorial experimental design with 3 replications under controlled conditions. PG-2018, Edessa, Flash, BA-308 cotton varieties were used as materials in the experiment and the salinity doses (NaCI applications) were adjusted as 0 (control), 4, 8, 12 dS/m. At the end of a 10-day germination period, the plants; Root length, petiole length, seedling height seedling fresh weight, seedling dry weight and germination rate values were investigated. According to the data obtained as a result of the experiment, statistically significant differences were found between the varieties and doses in all the properties examined. Variety x dose interaction was insignificant only in seedling dry weight. All properties were negatively affected by increasing salt concentrations in the study. It is among the results that the most resistant variety to salt stress was PG-2018 and tolerates a dose of 4 dS/m.Öğe Bazı yerel mısır genotiplerinde SSR yöntemiyle genetik çeşitliliğin belirlenmesi(2017) Baran, Nurettin; Temiz, Mefhar Gültekin; Kurt, FıratBu çalışmada Karadeniz Bölgesinden toplanmış olan 32 yerel popülasyon arasındaki genetik çeşitlilik SSR markör tekniği analiz edilmiştir. Araştırmada kullanılan 22 primerden sadece 14 tanesi bant vermiş ve toplam 42 allel elde edilmiştir. SSR lokusu başına ortalama 3 allel olduğu saptanmıştır. En fazla allel umc1571 lokusundan (6); en az allel ise phi022 (1) lokusundan elde edilmiştir. En büyük PIC değeri umc 1450 lokusundan 0.95 olarak elde edilmiştir. En düşük PIC değeri ise PHİ032 lokusundan 0.43 olarak elde edilmiştir. Çorum ve Trabzon genotiplerinin birbirine genetik olarak en yakın popülasyonlar olduğu buna karşın Artvin ve Rize popülasyonlarının birbirlerine genetik olarak en uzak olan popülasyonlar olduğu saptanmıştır.Öğe The determination of tolerances of some cotton genotypes against wilt disease (Verticillium dahliae Kleb.) under the ecological conditions of Diyarbakir(Turkish Science and Technology Publishing, 2022) Baran, Nurettin; Andırman, Mehtap; Shimira, Flavien; Nyirahabimana, Fildaus; Temiz, Mefhar GültekinVerticillium wilt disease in cotton is a soil-borne disease that causes significant yield losses, and it is therefore important to identify disease-resistant genotypes. Current study was carried out in the experimental field of the Faculty of Agriculture, Dicle University (Diyarbakir) in the year 2020 to determine the tolerance levels of some cotton genotypes which contain different characteristics to wilt disease (Verticillium dahliae Kleb.). The experiment area has been naturally infected with Verticillium wilt pathogens. A total of 124 cotton genotypes, including four controls (DP-499, Edessa, ST-468, ES-1), were arranged in five blocks according to the Augmented Design. The indices of stem cross-section and the leaf-disease severity were found significant. The leaf-disease severity varied between 0.12-3.09 at 50-60 % at boll opening period, whilst stem cross-section values ranged between 0.36-2.30. Moreover, a positive correlation was found between the indices. In conclusion, G7, G12, G21, G22, G23, G41, G51, G55, and G101 were determined as tolerant genotypes to Verticillium wilt in terms of examining two parameters.Öğe Exploring genetic diversity and population structure of Turkish black sea region maize (Zea mays L.) germplasm using SSR markers(Erzincan Binali Yıldırım Üniversitesi, Fen Bilimleri Enstitüsü, 2022) Baran, Nurettin; Nadeem, Muhammad Azhar; Yılmaz, Abdurrahim; Andırman, Mehtap; Kurt, Fırat; Temiz, Mefhar Gültekin; Baloch, Faheem ShehzadThis study aimed to investigate the genetic diversity and population structure of 32 local maize genotypes collected from the Black Sea Region of Turkey using SSR markers. 14 most polymorphic primers yielded a total of 42 bands. An average of 3 alleles per SSR primer was detected, and the number of alleles varied from 1 (phi022) to 6 (umc1571). The unweighted pair-group method with arithmetic means (UPGMA) clustering divided maize accessions into three main populations. According to Nei's genetic distances, DZ-M-145 (Corum) and DZ-M-20 (Trabzon) genotypes were the closest (0.03) genetically related populations, while DZ-M-68 (Artvin) and DZ-M-55 (Rize) were the most genetically distant (0.63) populations. The study identified molecular genetic diversity not mentioned for maize plants from the Black Sea.Öğe Exploring the genetic diversity and population structure of upland cotton germplasm by iPBS-retrotransposons markers(Springer Science and Business Media, 2023) Baran, Nurettin; Shimira, Flavien; Nadeem, Muhammad Azhar; Altaf, Muhammad Tanveer; Andırman, Mehtap; Baloch, Faheem Shehzad; Temiz, Mefhar GültekinBackground: Upland cotton is one of the utmost significant strategic fiber crops, and play a vital role in the global textile industry. Methods and results: A total of 128 genotypes comprised Gossypium hirsutum L, Gossypium barbadense L., and pure lines were used to examine genetic diversity using iPBS-retrotransposon markers system. Eleven highly polymorphic primers yielded 287 bands and 99.65% polymorphism was recorded. The mean polymorphism information content was estimated at 0.297 and the average diversity indices for the effective number of alleles, Shannon’s information index, and overall gene diversity were 1.481, 0.443, and 0.265, respectively. The analysis of molecular variance (AMOVA) revealed that 69% of the genetic variation was within the population. A model-based STRUCTURE algorithm divided the entire germplasm into four populations and one un-classified population, the genotypes G42 (originating in Egypt) and G128 (originating in the United States), showed the highest genetic distance (0.996) so these genotypes could be suggested for breeding programs as parental lines. Conclusions: This is the first investigation using an iPBS-retrotransposon marker system to examine the genetic diversity and population structure of upland cotton germplasm. The rich diversity found in upland cotton germplasm could be exploited as a genetic resource when developing breeding programs and could also help with efforts to breed cotton around the world. These findings also show the applicability and effectiveness of iPBS-retrotransposons for the molecular characterization of cotton germplasm.Öğe Genetic Insights into Poaceae Forages:A Review of Current Marker Studies(2023) Bayhan, Bora; Baran, NurettinForage variety development for diversified environmental conditions may benefit from the use of genomic-based breeding procedures. In today's conditions, molecular markers are used by researchers in this field to track loci and genome regions in crop breeding studies. Although earlier characterization efforts yielded useful information, morphological traits and RAPD markers have limitations when used together for genetic diversity research. Different combinations of methodologies are required for diversified aims to study different forage species at the genetic level and to connect micro level traitsto macro level traits.
