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Öğe Characteristic ultrasound appearance of a rare testis tumor: Bilateral multiple large-cell calcifying Sertoli cell tumor(John Wiley and Sons Inc., 2023) Ayaz, Ercan; Okur, Nurettin; Çelebi, Canan; Özekinci, Selver ÖzşenerA 16-year-old boy was admitted to the pediatric outpatient clinicwith a long-standing firmness in the scrotum. On examination, smallmasses were palpated in both testicles. The hormone levels (testos-terone:5.09ng/mL,estradiol:29.8pg/mL,FSH:6.46mIU/mL,andLH: 6.34 mIU/mL) and tumor markers (AFP: 0.9 ng/mL,β-Hcg: 0.1,and LDH: 148 U/L) were within the normal range. On ultrasound(US), bilateral multiple testicular masses, including coarse calcifica-tions, were seen.Öğe The comparison of endogenous angiogenesis inhibitors in normotensive and preeclamptic placentas: an immunohistochemical study(Informa Healthcare, 2014) Ayaz, Ercan; Nergiz, Yusuf; Tunik, Selcuk; Yalinkaya, AhmetObjective: Recently, it has been reported that endogenous angiogenesis inhibitors play a key role in the pathophysiology of preeclampsia. Thrombospondin-1, angiostatin and vasostatin are endogenous angiogenesis inhibitors and so far have not been shown in placenta at the immunohistochemical level. The aim of this study was to compare staining patterns of these endogenous angiogenesis inhibitors immunohistochemically in placentas of preeclamptic and normotensive pregnant women. Methods: Into the study, placentas from 20 preeclamptic and 20 normotensive pregnant women were included. Central and peripheral tissues were taken from both sides of placentas. Paraffin tissue blocks were prepared and stained for immunohistochemical analysis. Slides were evaluated for syncytiotrophoblasts, cytotrophoblasts, extra-villous throphoblasts and decidual cells. The degree of staining of slides were classified as negative, weak, moderate and strong. Results: Samples from preeclamptic patients were compared with those of normotensive. Staining of thrombospondin-1 was observed to increase in decidual cells, syncytiotrophoblasts in chorionic and stem villi and stroma of stem villi. Increased staining of thrombospondin-1 was only significant in the amniotic epithelium of the central sections. In addition, increased staining intensity of angiostatin was detected in the amniotic epithelium and chorionic plate of central sections of placenta. In peripheral sections, staining of angiostatin also increased in decidual cells but decreased in chorionic plate. Vasostatin staining in decidual cells, decidual stroma and chorionic villous stroma from peripheral sections decreased, but any difference was not observed in the central sections. Conclusion: Our results suggest that thrombospondin-1, angiostatin and vasostatin may play a role in the pathophysiology of preeclampsia. Further molecular studies are required to understand this role.Öğe The effect of extremely low frequency magnetic field on heart tissue iron density(Modestum Publishing Ltd., 2011) Söker, Sevda; Sert, Cemil; Deniz, Mustafa; Ayaz, Ercan; Tunik, Selçuk; Nergiz, YusufAmaç: Bu histolojik çalışmanın amacı; aşırı düşük frekanslı, düşük yoğunluklu manyetik alanın kalp dokusu demir yoğunluğu üzerindeki etkilerini araştırmaktır. Gereç ve yöntem: Bu çalışmada; sham, kontrol ve deney grubu olarak üçe bölünen 45 Spraque Dawley erkek rat kullanıldı. Deney grubu günde üç saat 14 gün boyunca metakrilat kutularda 0.25 mT oldukça düşük frekanslı manyetik alana (ELF-MF) maruz bırakıldı. Sham grubu, ELF-MF uygulaması dışında deney grubuna benzer şekilde uygulamaya maruz bırakıldı. Kontrol grubu ratlara hiçbir şey uygulanmadı ve fareler çalışma peryodu boyunca yaşam sikluslarını kafes içinde tamamladı. Yapılan işlem sonrasında, 50 mg/kg intramuskuler ketalar anestezisi uygulanarak ratlar sakrifiye edildi Kalp dokuları hemen %10 nötral formalinde fikse edilerek parafin bloklara gömüldü. Kalp dokusunun histolojik örnekleri Hematoksilen-Eosin ve demir pigmentleri için Perls’ Prussian blue boyası ile boyandı. Nikon DS-2MV fotomikroskopta histolojik preparatlar fotoğraflandı. Bulgular: Kontrol, sham ve deney grubumuzun histolojik yapısı normal gözlendi. Kontrol, sham ve deney grubu arasında kalp dokusu demir boyaması açısından farklılık gözlenmedi. Sonuç: Çalışmamız sonucunda, kontrol ve ELF-MF (deney) grubu arasında farklılık saptamadık. Bu çalışmada, ELF-MF’ye maruz kalan farelerin kalp dokusunda demir boyaması ile değişiklik olmadığı saptandı. Klin Deney Ar Derg 2011;2(2):144-8.Öğe Effects of Extremely Low Frequency Magnetic Field on Pulp Tissue of Rats(Medwell Online, 2011) Kaya, Sadullah; Celik, M. Salih; Akdag, Mehmet Zulkuf; Adiguzel, Ozkan; Yavuz, Izzet; Nergiz, Yusuf; Ayaz, ErcanThis study aims to investigate the effect of Extremely Low Frequency Magnetic Field (ELF-ME) on dental pulp of ovariectomized rats. About 75 female Wistar-albino rats were randomly divided into 5 groups: cage-control, ovariectomy, ELF-MF exposure, ELF-MF exposure with strontium ranelate treatments and ovariectomy application, ELF-MF exposure with ovariectomy application. All groups except cage-control and ovariectomy groups were exposed to 1.5 mT ELF-MF exposure throughout 6 months, 4 h a day. After all applications, pulp tissues of rat teeth were investigated under light microscope. Between the groups, histological differences in collagen fibrils, fibroblasts, blood vessels and odontoblasts that create the pulp were found. We concluded that osteoporosis affected the odontoblasts and fibroblasts in rat dental pulp, ELF-MF exposure did not resolve the histopathological effect due to osteoporosis but enhanced this pathological effect, strontium ranelate was more efficient to eliminate the effects of osteoporosis than ELF-MF application.Öğe EFFECTS OF HYPERBARIC OXYGEN THERAPY ON RAT NASAL MUCOSA(Diagnosis Press Ltd, 2012) Yorgancilar, Ediz; Tunik, Selcuk; Deveci, Engin; Gun, Ramazan; Bakir, Salih; Kinis, Vefa; Ayaz, ErcanThe objective of this study was to evaluate the histopathologic effects of hyperbaric oxygen (HBO) therapy on the rat nasal mucosa. Twelve adult Sprague-Dawley rats, each weighing 180-220 g, were used as experimental animals. The rats were divided into HBO (hyperbaric oxygen) and control group. The rats in the HBO group (n = 6) were placed into a 20-liter HBO chamber (2.5 atmospheres absolute LAW, 25-26 degrees C with 100 % oxygen) for 90 min per day. The rats received hyperbaric oxygen over a period of 7 days. The rats in the control group (n = 6) were not given HBO. All animals were sacrificed at the end of the study, and nasal tissue samples were prepared. The sections were stained with Haematoxylin and Eosin (H-E), Periodic acid-Schiff (PAS) and Trichrome-Masson to observe the under a light microscope. Immunoreactivity of pseudostratified epithelial cells of the nasal mucosa was assessed with E-cadherin expression by immunohistochemical staining. There were significant differences in the average histopathological score between the groups exposed and non-exposed to HBO. In the HBO group, degenerative changes in epithelial cells were observed. The goblet cells showed expansion of their structure. Mononuclear lymphocyte infiltration, dilation of blood vessels, and hemorrhage were observed in considerable areas of connective tissue. In the immunohistochemical evaluation of E-cadherin expression, there were no significant differences between the two groups. Biotechnol. & Biotechnol. Eq. 2012, 26(6), 3394-3396Öğe Effects of Pulsed and Sinusoidal Electromagnetic Fields on MMP-2, MMP-9, Collagen Type IV and E-cadherin Expression Levels in the Rat Kidney An Immunohistochemical Study(Sci Printers & Publ Inc, 2013) Tunik, Selcuk; Ayaz, Ercan; Akpolat, Veysi; Nergiz, Yusuf; Isen, Kenan; Celik, M. Salih; Seker, UgurOBJECTIVE: To investigate the role of extremely low frequency pulsed and sinusoidal electromagnetic fields on kidney tissues. STUDY DESIGN: Twenty-seven male Wistar albino rats were used. The rats were divided into 3 groups (n = 9): control group, sinusoidal electromagnetic field (SEMF) group, and pulsed electromagnetic field (PEMF) group. The SEMF and PEMF groups (pulse time 25 mu sn, pulse frequency 50 Hz) were subjected to 1.5 mT, 50 Hz, exposure 6 hours a day, 5 days a week for 28 days in methacrylate boxes. Formalin-fixed, paraffin-embedded kidney tissue sections were stained with hematoxylin-eosin, Gomori and periodic acid-Schiff. In addition, matrix metalloproteinase-2 (MMP-2) and 9 (MMP-9), E-cadherin and collagen type IV expression levels were examined immunohistochemically. RESULTS: Thickening of glomerular basement membranes was evident in electromagnetic fields, especially in the SEMF group. In addition, expression levels of E-cadherin were decreased with electromagnetic field (EMF) exposure. The expression level of MMP-9 increased, and MMP-2 and collagen type IV expression levels were not altered with EMF exposure. CONCLUSION: Both EMFs changed the molecular component of the kidney adversely.Öğe Effects of Pulsed and Sinusoidal Electromagnetic Fields on Rat Testes(Sci Printers & Publ Inc, 2020) Tunik, Selcuk; Baran, Ozlem Pamukcu; Akpolat, Veysi; Ayaz, Ercan; Nergiz, Yusuf; Celik, M. Salih; Yumusak, OzkanOBJECTIVE: To investigate the role of 50-Hz extremely low frequency pulsed (PEMF) and sinusoidal (SEMF) electromagnetic fields on the testis using a rat model. STUDY DESIGN: Twenty-seven male Wistar albino rats were used. The rats were divided into three groups (n=9 each): sham group, SEMF group, and PEMF group. The SEMF and PEMF groups (pulse time: 20 milliseconds, pulse frequence: 50 Hz) were subjected to 1.5 mT, 50 Hz exposure 6 hours a day, 5 days a week for 28 days in methacrylate boxes. Formalin-fixed, paraffin-embedded tissue sections were stained with H-E and PAS. In addition, E-cadherin and type IV collagen expressions were examined immunohistochemically. RESULTS: Seminiferous tubule basement membranes decreased with EMFs, especially those treated with SEMF. In addition, expression levels of E-cadherin on seminiferous epithelium decreased in PEMF and SEMF groups. The expression level of type IV collagen was also decreased in perivascular and seminiferous tubule basement membrane as compared to that in the sham group. CONCLUSION: PEMF and SEMF have adverse effects on the testis at a histopathological level. EMF leads to a decrease in E-cadherin and type IV collagen expression levels.Öğe The Effects of Systemic Use of Nicotine on the Rat Nasal Mucosa: a Histopathologic and Immunohistochemical Study(Soc Chilena Anatomia, 2012) Yorgancilar, Ediz; Tunik, Selcuk; Deveci, Engin; Gun, Ramazan; Bakir, Salih; Kinis, Vefa; Ayaz, ErcanThe objective of this study was to evaluate the histopathologic effects of systemic use of nicotine on the rat nasal mucosa. Twelve adult Sprague-Dawley rats weighing 180-220 g, were used as experimental animals. The rats were divided into Nicotine and control groups. The rats of Nicotine groups (n=6) were administered 2mg/kg Nicotine sulphate for 28 days. The rats of control group (n=6) were only administered 1,5 ml physiologic saline solution subcutaneously for 28 days. All animals were sacrified at the end of the study and nasal tissue samples were removed and prepared for histologic examination. The sections were stained with Hematoxylin and Eosin (H-E) and Periodic acid-Schiff(PAS) and Trichrome-Masson were observed under light microscope. E-cadherin immunreactivity of pseudostrafied epithelial cells of nasal mucosa was assessed by immunohistochemical staining. There were significant differences in average histopathological score between the groups treated and non-treated to nicotine. In nicotine group, degenerative change of epithelial cells and hypertrophy of goblet cells were observed. Leukocytes infiltration was observed in significant areas of connective tissue. E-cadherin expression was significantly decreased in epithelial cells of the nasal mucosa of Nicotine group.Öğe Endojen anjiyogenez inhibitörlerinin normotansif ve preeklamptik plasentalardaki ekspresyonlarının immünohistokimyasal olarak karşılaştırılması(2016) Ayaz, Ercan; Nergiz, YusufPreeklampsi gebeliğe özgü hipertansif bir hastalık olup, plasentadan kaynaklanan ve yaygın maternal endotel disfonksiyonuyla karekterize sistemik bir sendromdur. Son zamanlarda preeklampsi patofizyolojisinin etiyolojisinde antianjiyogenetiklerin anahtar rol aldığı bildirilmektedir. Bu çalışmanın amacı daha önce preeklamside etkinlikleri ortaya konulmamış olan trombospondin-1,anjiyostatin, vazostatin gibi antianjiogenetiklerin preeklamptik ve normotansif anne plasentalarındaki ekspresyonlarını immünohistokimyasal olarak karşılaştırmaktır. Çalışma için 20 preeklampsi, 20 normotansif (kontrol) gebe plasentası kullanıldı. Çalışmada kullanılan plasentaların maternal ve fetal yüzlerinden, biri periferden diğeri ise sentralden olmak üzere iki doku parçası 1X1cm3 boyutlarında alındı. Ardından rutin parafin doku takibi yapıldı. Parafin bloklardan elde edilen kesitler histokimyasal inceleme amacıyla H-E, Trikrom Masson, PAS boyaları ve immünohistokimyasal inceleme için ise Trombospondin-1, Angiostatin, Vazostatin immün boyamaları yapılarak histopatolojik değerlendirmeler yapıldı. Preeklamtik plasentaların histopatolojik incelemesinde perivillöz fibrin birikimi, intervillöz aralıkta artış, sinsityal düğüm artışı, bazal membranda kalınlaşma, kapiller dilatasyon, kapillerlerde konjesyon, damar duvarlarında kalınlaşma ve damar lümeninde obliterasyon tespit edildi. Matriks kökenli antianjiyojenik bir molekül olan trombospondin-1’in ekspresyonu plasentanın neredeyse tamamında olurken, sinsityotrofoblastlarda ve desidual hücrelerde sık gözlenen bu ekspresyonun preeklamptiklerde artmış olduğu izlendi. Non-matriks kökenli antianjiyojenik bir molekül olan anjiyostatin, sinsityal düğüm ve köprülerde eksprese olurken iki grup arasında bir farklılık bulunmadı. Vazostatinde desidual hücrelerde, desidual stromada, sinsityotrofoblast hücrelerinde, immün reaksiyon izlendi. Kontrol ve preeklampsi gruplar arası karşılaştırma yapıldığında herhangi bir farklılık saptanmadı. Sonuç olarak antianjiyogenik ajanlardan trombospondin-1, anjiyostatin ve vazostatin, preeklampsi patofizyolojisinde rol aldığı, bunlardan en önemlilerinin trombospondin-1 ve anjiyostatin olduğu, bu moleküllerin etkilerinin merkezinde en çok desidual hücreler ve sinsiyotrofoblast hücrelerinin yer aldığı gözlendi. Anahtar Kelimeler: Preeklampsi, Trombospondin-1, Vazostatin, Anjiyostatin, PlasentaÖğe The Prophylactic Effects of Folic Acid and Vitamin E against Valproic Acid During Fetal Thymus Development: an Ultrastructural Study(Soc Chilena Anatomia, 2011) Baran, Ozlem Pamukcu; Tunik, Selcuk; Akkoc, Hasan; Devieci, Engin; Ayaz, Ercan; Soker, Sevda; Tas, Sevgi KalkanliTo evaluate histopathologic differences in the thymus of Wistar Albino rat fetuses prenatally exposed to valproic acid (VPA), folic acid (FA) and vitamin E (Vit-E). VPA (400 mg/kg), FA (400 mcg/kg) and Vit -E (250 mg/kg) were administered to rats on each of gestation days 8,9 and 10. The fetuses (n:24) were divided into four groups: control, VPA, VPA+Vit-E and VPA+FA groups. On the 20th day of gestation, all pregnant rats were sacrificed and the fetuses were extracted. Thin sections from thymus of live fetuses were stained with uranyl acetate-lead citrate and were examined under transmission electron microscope. The histopathological findings of control group was normal. In VPA group, it showed extensive degenerative changes by VPA were on all tissue compartments when compared to controls. In VPA-FA group, vacuoles, mitochondrial cristalysis and swelling were decreased in cytoplasm. In VPA-Vit-E group, lipid storage and vacuolization were observed. Mitochondrial cristalysis decreased. Our aim in the present study is to analyze histopathological changes which may occur in a high risk experimental model after giving of VPA. In addition, protective roles of the administration of FA and Vit-E are assessed.Öğe Santral Puberte Prekokslu Her Olguya Kraniyal Manyetik Rezonans Görüntüleme Gerekli Mi?(2023) Yıldırım, Ruken; Unal, Edip; Ayaz, ErcanAmaç: Puberte prekoks kız çocuklarında 8, erkek çocuklarında 9 yaşından önce sekonder seks karakterlerinin gelişmesi olarak tanımlanmaktadır. Santral ve periferik puberte prekoks diye ikiye ayrılmaktadır. Santral puberte prekoks (SPP) kız çocuklarında daha sık görülür ve genellikle idiyopatik nedenlidir. Altta yatan patolojiyi aydınlatmak için 6 yaşından önce başlayan SPP’li olgulara kraniyal manyetik rezonans görüntüleme (MRG) çekilmesi önerilir iken, 6-8 yaş arasındaki olgulara çekilmesi tartışmalıdır. Bu çalışmada SPP tanısı konulan kız olgularda intrakraniyal lezyonların sıklığını ve kraniyal MRG sonuçlarını değerlendirmeyi amaçladık. Gereç ve Yöntem: Çalışmaya SPP'li 121 kadın hasta dahil edildi. Hastaların tıbbi kayıtlarının incelenmesinde antropometrik ölçümler, laboratuvar bulguları ve kraniyal MRG bulguları değerlendirildi. Bulgular: Çalışmaya alınan olguların ortalama yaşı 7.24 ± 1.04 idi. Yüz yirmi bir olgunun 7’sinde (% 5.8) SPP ile kesin ilişkili lezyonlar saptanırken, 12’sinde (% 9.9) SPP ile muhtemel ilişkili lezyonlar, beşinde ( %4.1) ise SPP’ye yol açmayan tesadüfi bulgular (insidentaloma) tespit edildi. Kraniyal patoloji saptanan tüm hastalarda nörolojik bulgu mevcut değildi. İntrakraniyal lezyon saptanan olguların tümü 6-8 yaş aralığında idi. Sonuç: Çalışmamızda önceki birçok çalışmanın aksine, 6 yaşından sonra başlayan SPP olgularında kraniyal patoloji sıklığının az olmadığı gösterilmiştir. Bu nedenle 8 yaşından önce SPP tanısı konulan tüm kız olgulara nörolojik bulguların varlığına bakılmaksızın kranial MRG yapılmasını önermekteyiz.
