DSpace Arşivi :: by Yazar "Allender, Rebecca" değerine göre listeleniyor

Listeleniyor 1 - 1 / 1

The toxicity of dithiocarbamate fungicides to soil nematodes, assessed using a stress-inducible transgenic strain of Caenorhabditis elegans
(John Wiley and Sons Inc., 1999) Güven, Kemal; Power, Rowena S.; Avramides, Sophia; Allender, Rebecca; de Pomerai, David I.; 0000-0002-0181-3746
The dithiocarbamate fungicides maneb and mancozeb induce a short-term stress response in a transgenic Caenorhabditis elegans strain (PC72) carrying a reporter lacZ gene under the control of a homologous heat shock (hsp16) promoter. This response can be readily monitored as induced beta-galactosidase activity, either by in situ staining or by a quantitative fluorometric enzyme assay. Particularly strong responses are induced by mancozeb (three- to fivefold above controls at 500 pg mL(-1)), causing acute toxicity at concentrations comparable to those recommended for field application (2 mg mL(-1)). Although much of this fungicide is adsorbed by soil, sufficient (ca. 6%) enters the soil water compartment to cause mild stress in the transgenic worm assay. Among possible metabolites from mancozeb breakdown, neither Mn2+ nor ethylenethiourea (ETU) is particularly toxic even at 10% of the optimum mancozeb dosage. Stress responses to a range of other pesticides are also reported, and in several cases it is clear that a nontarget soil species there, transgenic C. elegans) may be sensitive to low-level contamination. (C) 1999 John Wiley & Sons, Inc.