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Öğe Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC-ESI-MS/MS with chemometric approach(Marmara University, 2021) Mokaddem-Daroui, Habiba; Mostefa, Maya Belhadj; Aydoğmuş-Öztürk, Fatma; Erol, Ebru; Öztürk, Mehmet; Ertaş, Abdulselam; Duru, Mehmet Emin; Kabouche, Ahmed; Kabouche, ZahiaThe fractions (Fr.1-5) of the hydromethanolic extract of Abies numidica cones were studied for their polyphenol constituents, antioxidant, and anticholinesterase activities. The beta-carotene-linoleic acid, cupric reducing power (CUPRAC), DPPH scavenging, and ABTS radical scavenging assays were used to determine antioxidant activity. Fr.3 exhibited the highest antioxidant activity in ABTS test (IC50: 1.29 mu g/mL), b-carotene-linoleic acid test (IC50: 18.6 mu g/mL) and CUPRAC (A(0.5): 33.8 mu g/mL) assays. Three fractions (Fr.2-4) promising antioxidant activity were analyzed using LC-MS/MS for their phenolic compositions. Taxifolin (155.9-2816.2 mu g analyte/g extract), hyperoside (353.0-2045.5 mu g/g), vanillin (1488.9-1529.9 mu g/g), tannic acid (1281.6-1416.8 mu g/g), rosmarinic acid (1063.1-1149.3 mu g/g), coumarin (683.0-772.6 mu g/g), quercetin (881.0 mu g/g), and catechin (277.7-400.2 mu g/g) were identified in the antioxidant fractions. Moreover, the anticholinesterase activity was also performed using the in vitro spectroscopic Ellman method. The antioxidant fractions exhibited moderate butyrylcholinesterase inhibitory activity. The multi-ware analysis was performed to understand the origin of bioactivities. According to principal component analysis, it is detected that the hyperoside, catechin, taxifolin, and tannic acid were responsible for the antioxidant activity. Therefore, the cones of Abies numidica can be considered as potent antioxidant and anticholinesterase sources in food and pharmaceutical industries, commercially.Öğe Chemical fingerprints and bioactivities of 12 Anatolian Achillea L. species by LC-MS/ MS with chemometric approach: novel phytonutrients, natural food preservatives and chlorogenic acid sources(Turkiye Klinikleri, 2022) Yılmaz, Mustafa Abdullah; Ertaş, Abdulselam; Yener, İsmail; Türkmenoğlu, Fatma Pınar; Ölmez, Özge Tokul; Öztürk, Mehmet; Altun, Muhammed; Çakır, Oğuz; Tarhan, Abbas; Boğa, MehmetThe objective of this study was to evaluate the biological activities and chemical fingerprint profiles of the extracts obtained from twelve Achillea L. species (A. lycaonica, A. biebersteinii (syn: A. arabica), A. kotschyi subsp. kotschyi, A. schischkinii, A. millefolium subsp. millefolium, A. sintenisii, A. setacea, A. teretifolia, A. wilhelmsii subsp. wilhelmsii (syn: A. santolinoides subsp. wilhelmsii), A. nobilis, A. goniocephala, A. spinulifolia). The antioxidant, enzyme inhibitory and cytotoxic effects were evaluated to investigate their bioactivity profiles. Furthermore, the total flavonoid and phenolic contents were determined and LC-MS/MS analysis was performed to reveal the phytochemical profile of the investigated extracts. A. kotschyi and A. nobilis species were detected to have very high antioxidant potential as well as high total phenolic content (260.00 ± 3.38 and 282.97 ± 3.14 μg of PEs mg extract–1, respectively). According to the LC-MS/ MS results, A. kotschyi and A. nobilis species were found to contain very high concentrations of chlorogenic acid (55812.20 and 46407 μg analyte g extract–1). Besides, the bioactivities and phenolic composition of these species were chemometrically analyzed using principal component analysis (PCA) and hierarchical clustering analysis (HCA) techniques. It has also been determined that Achillea species generally exhibit quite high cytotoxic activity against the HeLa cell line. The studied species showed high urease enzyme activities.Öğe A detailed biological and chemical investigation of sixteen schillea species' essential oils via chemometric approach(Wiley-V C H Verlag Gmbh, 2020) Yener, İsmail; Yılmaz, Mustafa Abdullah; Ölmez, Özge Tokul; Akdeniz, Mehmet; Tekin, Fetullah; Haşimi, Nesrin; Alkan, Mehmet Hüseyin; Öztürk, Mehmet; Ertaş, Abdülselam; 0000-0002-2093-4949; 0000-0001-8932-4535; 0000-0002-0988-9462; 0000-0002-4090-7227; 0000-0002-0761-6436; 0000-0002-2193-8386Representatives of the Achillea genus are widely used as foods or nutraceuticals. Considering the increasing demand for herbal dietary supplements with health promoting effects, the objective of this research was to evaluate the chemical composition and biological activities of the essential oils obtained from sixteen Achillea species (A. biebersteinii, A. wilhelmsii subsp. wilhelmsii, A. aleppica subsp. zederbaueri, A. vermicularis, A. monocephala, A. nobilis, A. goniocephala, A. sintenisii, A. coarctata, A. kotschyi subsp. kotschyi, A. millefolium subsp. millefolium, A. lycaonica, A. spinulifolia, A. teretifolia, A. setacea, and A. schischkinii). Anticholinesterase, antiurease, antityrosinase enzymes inhibition, antioxidant, antimicrobial, toxic and cytotoxic activities of obtained essential oils were investigated. DPPH activities were found to be very low in all studied samples, while ABTS and CUPRAC antioxidant activities were found to be moderate. In addition, all samples were found to have moderate anticholinesterase and antimicrobial effects. It has been determined that the studied species have low cytotoxicity and high toxicity. Besides, chemical composition of the essential oils were determined by GC/MS and the results were chemometrically analyzed. The chemometric analyses of Achillea species collected from nine different regions were accomplished by principal component analysis (PCA) and hierarchical cluster analysis (HCA) techniques. According to the PCA analysis, A. nobilis subsp. neilreichii was found to be different from all studied species in terms of essential oil composition. The major components found in these species were piperitone, camphor, alpha-terpinene, eucalyptol, artemisia ketone, endo-borneol, beta-eudesmol and verbenol. The fact that camphor was toxic and found in majority of the studied species stands out as a remarkable result.Öğe Determination and comparison of metal contents in simulated body fluid medium conditions of the plant species by flame atomic absorption spectrometry (FAAS)(Marmara University, 2022) Umaz, Adil; Aydın, Fırat; Ölmez, Özge Tokul; Fırat, Mehmet; Öztürk, Mehmet; Ertaş, Abdulselam; Aydın, IşılThe importance of plants in folk medicine and scientific studies is increasing day by day. In this context, the plants pose a danger to human health when they are eaten by the public, consumed as herbal tea or used as medicinal plants especially the toxic metals in their composition. For this reason, determining the macro, micro and toxic element content in the plants is important for health. In this study; the metal contents of root and aerial parts of nine different Salvia species were determined and the chemometric evaluation of the obtained results was made. In the principal component analysis (PCA) made with 20 elements of Salvia species, it was determined that the first two principal components explained 62.60% of the variance and the first six principal components explained 92.70%. When PCA and hierarchical cluster analysis (HCA) results are evaluated; the parts of Salvia species root and aerial parts were determined that were not clearly separated and there was no regional grouping. In addition, Salvia species prepared as herbal tea was left to different simulated body fluid medium conditions, and the changes in metal contents were examined. It was determined that were taken up Cr, Cd, Pb, Cu, Zn metals in the simulated saliva fluid (SSF), Ni, Mn metals in the simulated gastric fluid (SGF), and Na, K metals in the simulated intestinal fluid (SIF). It was determined that Fe metal in almost all Salvia species was not taken from three simulated body fluid mediums. Thus, elements determined which were taken up in body fluid mediums.Öğe Essential oil, aroma, and fatty acid profiles of five endemic Salvia taxa from Turkey with chemometric analysis(Wiley-V C H Verlag GMBH, 2022) Ertaş, Abdulselam; Akdeniz, Mehmet; Yener, İsmail; Öztürk, Mehmet; Ölmez, Özge Tokul; Fırat, Mehmet; Kolak, UfukThe essential oil, aroma, and fatty acid compositions of Salvia cerino-pruinosa var. cerino-pruinosa, S. cerino-pruinosa var. elazigensis, S. pseudeuphratica, S. rosifolia, and S. kurdica collected in 2015, 2016, and 2017 were analysed by GC-FID, GC/MS/Head Space and GC/MS. The results were evaluated chemometrically for principal component analyses and hierarchical clustering analyses using Minitab program. The main components of their essential oils have been determined as germacrene-D (41.79 % highest for these species), camphor (55.80 %), linalool (33.38 %), caryophyllene oxide (32.99 %), 1,8-cineole (26.30 %) and geraniol acetate (31.63 %), while for aroma 1,8-cineole (45.95 % highest for these species), camphor (58.54 %), D-limonene (40.83 %), linalool (27.67 %) and cis-linalool oxide (25.87 %). Oleic (72.65 % highest for these species), erucic (44.61 %), 2-palmitoleic (42.17 %) and nervonic (32.61 %) acids were determined as major components of their fatty acids. According to the PCA and HCA, the essential oil components of these Salvia taxa unaffected by the years, and accordingly, the Salvia taxa grouped among themselves.Öğe In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical fingerprints of five Moroccan seaweeds(Elsevier B.V., 2020) Grina, Fatiha; Ullah, Zain; Kaplaner, Erhan; Moujahid, Abderrahman; Eddoha, Rabiaa; Nasser, Boubker; Ertaş, Abdulselam; Öztürk, MehmetThe coastlines of Morocco have an abundant resource of diverse seaweeds but the bioactivity of many of these seaweeds is still unexplored. The aim of this study was to contribute to the evaluation of the antioxidant potential, using different in vitro systems and searching some fingerprints of phenolic constituents based on LC-MS/MS method of five seaweeds species obtained from Moroccan Atlantic Ocean: Bifurcaria bifurcata, Cystoseira humilis, Cystoseira stricta, Fucus spiralis and Gelidium sesquipedale. The total phenolic compounds, the antioxidant activity, anticholinesterase, tyrosinase inhibitory, urease inhibitory activities and the organic compounds were determined. The results showed that Fucus spiralis possessed the highest phenolic and flavonoid contents compared to the others. In addition, Fucus spiralis (IC50: 13.25 ± 0.9 µg/mL) and Cystoseira stricta (IC50: 13.58 ± 1.2 µg/mL) exhibited higher lipid peroxidation inhibitory activity. The highest1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50: 47.23 ± 3.8 µg/mL), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) dia-mmonium salt (ABTS) (IC50: 5.95 ± 0.6 µg/mL), and Cupric reducing antioxidant capacity (CUPRAC) (55.24 ± 0.04 µg trolox equivalents/mg) activity was measured in Fucus spiralis. Regarding metal chelating activity, Bifurcaria bifurcata was the best (IC50: 45.22 ± 0.9 µg/mL) compared to the others. The highest acetylcholinesterase (IC50: 28.92 ± 1.8 µg/mL), butyrylcholinesterase (IC50: 11.72 ± 2.8 µg/mL), tyrosinase (IC50: 6.19 ± 0.4 µg/mL) and urease (IC50: 10.96 ± 1.3 µg/mL) inhibitory activities was found in Fucus spiralis. According to LC-MS/MS results, quinic acid, malic acid, fumaric acid, gallic acid, protocatechuic acid, 4-OH-benzoic acid, hesperidin, were detected in ethanol extracts of the five Moroccan seaweeds. This study demonstrated that seaweeds, particularly Fucus spiralis and Cystoseira stricta could be a promising source of antioxidants and phytochemicals which can be used in cosmetics, pharmaceutical and as nutritional food supplement in food industries to deleterious free radical induced stress, disorder and diseases.Öğe Insight into isolation and elucidation of cytotoxic ergostanoids from the mushroom Sarcosphaera crassa (Santi) Pouzar: An edible mushroom(Elsevier Science INC., 2022) Ullah, Zain; Öztürk, Mehmet; Ertaş, Abdulselam; Wahab, Atia-tul; Ben Mansour, Riadh; Choudhry, M. IqbalSarcosphaera crassa is a mushroom consumed in Europe and Anatolia after being cooked well. The cytotoxic activity of the extracts of unbaked S. crassa against MCF7, HT29, HeLa cancer cell lines and toxicity against PDF fibroblast healthy cell lines were studied using MTT assay. Acetone and methanol extracts of the mushroom exhibited significant cytotoxic activity. Further investigation of cytotoxic extracts afforded two new fatty acid sterols (1-2), a new ergosterol glycoside (4), and seven known compounds, including a fatty acid sterol (3), a steroid glycoside (5), two ergostanoids (6-7) and three sugars (8-10). These compounds were identified as brassicasteryl heptadecanoate (1), brassicasteryl palmitoleate (2), brassicasteryl linoleate (3), brassicasterol beta-?-xylofuranoside (4), brassicasterol beta-?-glucoside (5), brassicasterol (6), ergosterol-endoperoxide (7), mannitol (8), erythritol (9) and turanose (10). Among them, 7 exhibited a moderate cytotoxic activity against HeLa (IC50: 70.1 +/-& nbsp; 2.0 mu g/mL) and high activity against HT29 (IC50: 38.8 +/- 0.9 mu g/mL), and MCF7 (IC50: 62.9 +/- 1.3 mu g/mL) cancer cell lines. Compounds 4, 5, and 6 also exhibited significant cytotoxic activity against HT29 and MCF7. Moreover, all compounds exhibited weak toxicity against PDF healthy cell lines. This study indicates the potential use of Sarcosphaera crassa as a natural source of cytotoxic ergostanoids, which can be considered a dietary supplement for cancer prevention.& nbsp;Öğe Lc-ms/ms simultaneous determination of 37 bioactive compounds in bunium crassifolium batt. And its biological activities(Marmara University, 2021) Souilah, Nabila; Bendif, Hamdi; Ullah, Zain; Hamel, Tarek; Djarri, Lakhdar; Öztürk, Mehmet; Ertaş, AbdulselamBunium crassifolium Batt. is an extremely rare species growing in Algeria. Its leaf or aerial part is used for flavoring and garnishing food. The aim of the current study was to determine 37 bioactive phytochemicals and biological evaluation of the methanol and methanol:water (70:30) extracts of B. crassifolium for the first time. A total of 10 phenolic acids and 8 flavonoids have been identified in the extracts of the aerial parts by LC–MS/MS. Chlorogenic, gallic and ferulic acids were the most abundant phenolic acids detected, while rhoifolin, quercitrin and rutin were the most abundant flavonoids. In addition, 3 non-phenolic organic acids (fumaric acids, quinic and malic) were detected and among them quinic and malic acids were the most abundant. Antioxidant activity was evaluted by six methods and the extracts showed significant activity. The total phenolic and flavonoid contents were determined and methanol:water (70:30) extract showed higher values which entails its greater antioxidant capacity. In addition, anticholinesterase (AChE and BChE) activity was evaluated for both extracts using Ellman method. Methanol extract showed a better anti-acetylcholinesterase activity than methanol:water (70:30) one. While, both extracts showed a weak anti-butyrylcholinesterase activity. Furthermore, the anti-tyrosinase activity was tested and the methanol:water (70:30) extract was promising and more potent. In conclusion, B. crassifolium could be used in food industries and pharmaceutical as a potential functional food ingredient.Öğe Pişirilmiş ve Pişirilmemiş Yenilebilir Ticari Mantar Türlerinden Antikanser ve Antioksidan Bileşiklerin İzolasyonu ve Yapılarının Tayinleri(2017) Duru, Mehmet Emin; Sıcak, Yusuf; Ertaş, Abdulselam; Öztürk, MehmetBu projede, Türkiye?de üzerinde çalışma yapılmamış, doğal olarak yetişen, çiğ tüketildiğinde zehirli olan ancak pişirildiğinde lezzetli bir gıda olan Sarcosphaera crassa (Santi) Pouz., Gyromitra esculenta (Pers.) Fr. ve Rhizopogon roseolus (Corda) Th. Fr. mantarları çalışıldı. Mantarın hem çiğ hemde pişirilmiş örneklerinden elde edilen aseton, metanol ve HWEP ile HWES ekstrelerin sitotoksik aktivitesi rahim ağzı (HeLa) ve meme (MCF-7) kanser hücre hatlarına karşı MMT yöntemiyle test edildi. Sarcosphaera crassa türünden SCN2, SCC2, SCN3 ve SCC3 ekstrelerinden saf madde izolasyonu kolon kromatografisi yöntemiyle silika jel adsorban kullanılarak yapıldı. İnce tabaka kromatografisi, preparatif ince tabaka kromatografisi ve recycle preperatif HPLC-UV-RI cihazı ile uygun adsorbanlar kullanılarak toplamda 11 tanesi yeni 20 madde saflaştırıldı. Sarcosphaera crassa?dan saflaştırılan maddelerin yapıları 1, 18 kodlu maddelerin ergosta- 5,22-dien 3?-ol, 2 kodlu maddenin ergosterol endoperoksit, 3 kodlu maddenin N-dodekanoil- N-(etilsülfinil) hekzadekanamit, 4 kodlu maddenin ergosta-5,22-dien 3-O-?-?-ksilofuranosil, 5 kodlu maddenin N-(1,3,4-trihidroksioktadekan-2-il) tetrakosanamit, 6 kodlu maddenin ergosta- 5,22-dien 3-O-?-?-glucopyranosil, 7 ve 20 kodlu maddelerin ?-D-glukopiranosil-(1?1)-?-D- glukopiranosit, 8 kodlu maddenin eritriyol, 9 kodlu maddenin ergosta-5,22-dienil 3-O-?- dekanoat, 10 ve 19 kodlu maddelerin N-(1,3,4-trihidroksikosan-2-il) tetrakasanamid, 11 kodlu maddenin ?-sorbitol, 12 kodlu maddenin di-4'-etiltridekan ftalat, 13 kodlu maddenin 1,2- dilinolenil gliserol, 14 kodlu maddenin sarcospin, 15 kodlu maddenin ergosta-5,22-dienil 3-O- ?-heptanoat, 16 kodlu maddenin ergosta-5,22-dienil 3-O-?-12'-cis-hegzadekenoat ve 17 kodlu maddenin mannitol olduğu belirlendi. Gyromitra esculenta türünden GEN2, GEC2, GEN3 ve GEC3 ekstrelerinden saf madde izolasyonu kolon kromatografisi yöntemiyle silika jel adsorban kullanılarak yapıldı. İnce tabaka kromatografisi, preparatif ince tabaka kromatografisi ve recycle preperatif HPLC-UV-RI cihazı ile uygun adsorbanlar kullanılarak toplamda 17 madde saflaştırıldı. Yapılan spektroskopik çalışmalarla, 21, 29, 32, 34 kodlu maddelerin ergosta-5,22-dien 3?-ol, 22 kodlu maddenin ergosta-5,22-dien 3-O-?-?-glukopiranozit, 23 ve 27 kodlu maddelerin ergosta-7,9,22-trien 3?- ol, 24 kodlu maddenin ergosta-7,9,22-trien 3-O-?-?- glukopiranozit, 25 kodlu madde etanediyal, 1,2-bis[2-formil-2-metilhidrazon, 28 ve 36 kodlu maddelerin adenozin, 30 kodlu maddenin 1-(1'-hidroksisinnamoil)-2-asetoksi-3-metoksi inositol, 31 kodlu maddenin ergosterol endo peroksit, 33 kodlu maddenin 5?,8?-epoksi-ergosta-6,22-dien 3?-ol, 35 kodlu maddenin urasil ve 37 kodlu maddenin mannitol olduğu belirlendi. Rhizopogon roseolus türünden RRN2 ve RRN3 ekstrelerinden saf madde izolasyonu kolon kromatografisi yöntemiyle silika jel adsorban kullanılarak yapıldı. İnce tabaka kromatografisi, preparatif ince tabaka kromatografisi ve recycle preperatif HPLC-UV-RI cihazı ile uygun adsorbanlar kullanılarak toplamda 4 tanesi yeni olan 7 madde saflaştırıldı. Yapılan spektroskopik çalışmalarla, 38 kodlu maddenin tetrakosanoil-4-(2',4'-di-tert-butilfenil)-4- metilpentanoat, 39 kodlu maddenin oktadesil-3-(3',5'-di-tert-butil-4-hidroksifenil)-propanoat, 40 kodlu maddenin roseolusin, 41 kodlu maddenin oktadekanol, 42 kodlu maddenin mannitol, 43 kodlu maddenin malik asit, 44 kodlu maddenin N-[3-hidroksi, 9-metil-1-glukopiranozil, trikosa- 4,8-dienil]-2-hidroksi dikosanamit olduğu belirlendi. Elde edilen maddelerin sitotoksik aktivitesi aynı metotlar ile belirlendi. Projeye konu olan mantarlardan elde edilen maddelerin bazılarının 50 ?M?ın altında sitotoksik aktivite gösterdiği tespit edildi. Proje sonuçları bu mantarların ülkemiz ekonomisine katma değer sağlayabileceği kanaatindeyiz. Ancak in vivo gibi ileri çalışmları yapılması gerekmektedir.Öğe Trace element analysis in some salvia species by inductively coupled plasma-mass spectrometry (Icp-ms) and chemometric approach(Marmara University, 2020) Tunay, Zeki; Yener, İsmail; Oral, Elif Varhan; Demirkoz, Aslı Barla; Ölmez, Özge Tokul; Fırat, Mehmet; Öztürk, Mehmet; Aydın, Işıl; Aydın, Fırat; Ertaş, AbdülselamIn this study, the stems, leaves, flowers, roots and mixed all parts of five Salvia species were analyzed for their trace element (Li, Be, V, Cr, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Ag, Cd, Cs, Ba, Hg, Tl, Pb, and U) contents using ICP-MS. The seeds, roots leaves, flowers, and mixed parts of each species were digested by concentrated nitric acid and hydrogen peroxide in a microwave by before ICP-MS the analysis. The accuracy and precision of the method were evaluated by CRM 1573a Tomato Leaves. Trace element contents in different parts of each sample were compared. Concentration of toxic elements (As, Cd, Hg and Pb) were lower than those declared by WHO, except Cr content. Cr content in the root sections of Salvia suffruticosa (SFR), S. hydrangea (SHR), S. trichoclada (STR), S. xanthocheila (SXR), leaf samples of S. kronenburgii (SKL) and S. xanthocheila (SXL). also the Cr content in the leaf sections of Salvia kronenburgii (SKL) Salvia xanthocheila (SXL) was found high. When consider the daily metal (Zn, Cu, Sr, Ba and Ni) needs It was concluded that these Salvia species can be nutritive sources. In addition, Salvia samples were classified by utilizing chemometric techniques such as Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA). While the first two main components explained 55.30% of the total variance, the first six main components explained 89.60% of total variance. © 2020 Marmara University Press.
