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Öğe Culture- and polymerase chain reaction-based detection of flavobacterium psychrophilum in naturally infected rainbow trout (Oncorhynchus Mykiss Walbaum, 1792) from trout farms(Hasan ÖNDER, 2023) Özcan, Filiz; Arserim, Neval Berrin; Ketani, M. Aydın; Aydın, Kasım; Sever, Nurdan Karacan; Özcan, Nida; Çelik, Burçak AslanThe present study aimed to detect Flavobacterium psychrophilum in fish samples collected from rainbow trout (Oncorhynchus mykiss Walbaum 1792) farms in the Southeastern Anatolia region of Türkiye by means of bacteriological culture and polymerase chain reaction and to investigate the antibiotic susceptibility of the causative bacteria. A total of 40 trout farms located in Diyarbakır, Adıyaman, Şanlıurfa, and Batman provinces were visited, and 1200 samples were examined. During January and February 30 fish with an average live weight of 200–250 g were collected from each farm. Samples were obtained from the liver, spleen, kidneys, and tissues following macroscopic laboratory examination of the specimens. Antibiotic treatment is the treatment of choice owing to the lack of an effective vaccine in the control of the disease. Therefore, it is important to rapidly identify the bacterial species and investigate its susceptibility to antibiotics. In this study, the causative bacteria were detected in 5 out of 40 farms. The causative bacteria infected the liver, kidney, and tissues. The sensitivity of Enrofloxacin (5 microgram (µg)), Florfenicol (30µg), Neomycin (5µg), Amoxicillin (25µg), Oxytetracycline (30µg), Erythromycin (10µg), Gentamycin (5µg), Streptomycin (5µg) and Nalidixic acid (10µg) were defined at chancing ratios. In conclusion, these bacteria were detected in regional farms, which should minimize the stress factors by avoiding overstocking and following the required hygiene rules.Öğe Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, Turkey(Universidad del Zulia, Facultad de Ciencias Veterinarias, 2023) Özcan, Filiz; Arserim, Neval Berrin; Ketani, Muzaffer Aydın; Aydın, Kasım; Sever, Nurdan Karacan; Özcan, Nida; Çelik, Burçak AslanThe present study aimed to detect Yersinia ruckeri bacteria in fish samples collected from the rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) farms in the South Eastern Anatolia Region of Turkey through bacteriological culturing and polymerase chain reaction. Separate visits were made to trout farming facilities in Diyarbakır, Adıyaman, Şanlıurfa, and Batman in January and February 2021, and 30 fish with an average live weight of 200–250 g were collected from each facility. A total of 1,200 samples were taken from 40 trout farms. Liver, spleen, kidney, and tissue specimens were collected from rainbow trout. Yersinia ruckeri was isolated from the kidney, liver and muscle tissue of fish on McConkey Agar for bacteriological examinations. Isolated strains were identified by MALDI–TOF and PCR. It was detected the bacteria in the liver, kidney, and muscle tissue samples of fish from 23 out of 40 farms. In conclusion, these bacteria were detected in local farms. These farms must implement vaccination, minimize stress factors affecting the fish, and avoid overstocking. The best methods to protect against infection include avoiding overstocking, low oxygen levels, and underqualified labor force.Öğe First detection of Ehrlichia chaffeensis, Ehrlichia canis, and Anaplasma ovis in Rhipicephalus bursa ticks collected from sheep, Turkey(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2024) Ayan, Adnan; Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Kılınç, Özlem Orunç; Akyıldız, Gürkan; Yılmaz, Ali Bilgin; İpek, Duygu Neval SayınAnaplasmosis and ehrlichiosis are important tick -borne rickettsial diseases of medical and veterinary importance that cause economic losses in livestock. In this study, the prevalence of Anaplasma ovis, Ehrlichia canis and Ehrlichia chaffeensis was investigated in ticks collected from sheep in various farms in Van province, which is located in the Eastern Anatolian Region of Turkey. The ticks used in this study were collected by random sampling in 26 family farm business in 13 districts of Van province. A total of 688 ticks were collected from 88 sheep and 88 tick pools were created. All ticks identified morphologically as Rhipicephalus bursa. Phylogenetic analysis of Chaperonin and 16S rRNA gene sequences confirmed A. ovis, E. canis and E. chaffeensis in this study. Of the 88 tick pools tested, 28.41% (25/88) were positive for at least one pathogen. Anaplasma DNA was detected in five of the 88 pools (5.68%), E. canis DNA was detected in 19 of the 88 pools (21.59%), and E. chaffeensis DNA was detected in one of the 88 pools (1.14%) of R. bursa ticks. To our knowledge, this is the first report describing the presence of A. ovis, E. canis, and E. chaffeensis in R. bursa ticks collected from sheep in Turkey. Further studies are needed to investigate other co -infections in sheep in Turkey.Öğe Investigation of seroprevalence of toxoplasma gondii in cattle in siirt province in Turkey(Agricultural Research Communication Centre, 2018) Çelik, Özgür Yaşar; İpek, Duygu Neval Sayın; Çelik, Burçak Aslan; Irak, Kıvanç; Akgül, GülşahToxoplasmosis is an important zoonotic disease, which is caused by Toxoplasma gondii and is quite common in the world, found in all mammals including humans, poultry, and reptiles. It has been reported that; adult cattle do not have toxoplasmosis clinically, and it is possible they pass the parasite through the placenta to the fetus, and that some infected animals give birth to aborted or infected calves. This study was conducted to determine T. gondii seroprevalence in cattle of Siirt region in Turkey and seropositivity was determined in blood samples of 300 cattle by using ELISA. Results showed that 53 (18%) of the samples were seropositive, while 247 (82%) of the samples were seronegative. In addition, there was a significant difference between their average age groups.Öğe Prevalence and genotypes of Giardia duodenalis in shelter dogs of Southeastern Türkiye(Urmia University - Faculty of Veterinary Medicine, 2023) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Koçhan, Akın; Ayan, Adnan; Kılınç, Özlem Orunç; Akyıldız, Gürkan; İrak, KıvançGiardia duodenalis is a protozoan parasite found in humans and several mammals. This parasite spreads worldwide and is generally recognized as a zoonotic agent being reported to be one of the most common causes of diarrhea in humans and animals. In this study, it was aimed to determine the prevalence and genotypes of G. duodenalis in shelter dogs in Diyarbakır province being located in the southeastern Anatolia region of Türkiye. Native-Lugol method and nested polymerase chain reaction analyses of 100 fecal samples showed a prevalence of 3.00 and 4.00%, respectively. The prevalence was higher in females and in those younger than 1 year. Sequence analysis revealed the presence of zoonotic assemblage B, assemblage D and assemblage E. The detection of zoonotic assemblage B in this study suggests that dogs may be a reservoir for human giardiasis. Further molecular research is needed to determine the genotype diversity of Giardia as well as its possible role in the transmission of this parasite to humans.
