HN1 is enriched in the S-phase, phosphorylated in mitosis, and contributes to Cyclin B1 degradation in prostate cancer cells

Basit Öğe Kaydı
dc.authorid0000-0002-4337-9327en_US
dc.contributor.authorJaved, Aadil
dc.contributor.authorÖzduman, Gülseren
dc.contributor.authorVarışli, Lokman
dc.contributor.authorÖztürk, Bilge Esin
dc.contributor.authorKormaz, Kemal Sami
dc.date.accessioned2023-01-30T13:20:10Z
dc.date.available2023-01-30T13:20:10Z
dc.date.issued2023en_US
dc.departmentDicle Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.descriptionBu bir erken erişim sürümüdür.en_US
dc.description.abstractHN1 has previously been shown as overexpressed in various cancers. In Prostate cancer, it regulates AR signaling and centrosome-related functions. Previously, in two different studies, HN1 expression has been observed as inversely correlated with Cyclin B1. However, HN1 interacting partners and the role of HN1 interactions in cell cycle pathways have not been completely elucidated. Therefore, we used Prostate cancer cell lines again and utilized both transient and stable inducible overexpression systems to delineate the role of HN1 in the cell cycle. HN1 characterization was performed using treatments of kinase inhibitors, western blotting, flow cytometry, immunofluorescence, cellular fractionation, and immunoprecipitation approaches. Our findings suggest that HN1 overexpression before mitosis (post-G2), using both transient and stable expression systems, leads to S-phase accumulation and causes early mitotic exit after post-G2 overexpression. Mechanistically, HN1 interacted with Cyclin B1 and increased its degradation via ubiquitination through stabilized Cdh1, which is a co-factor of the APC/C complex. Stably HN1- expressing cells exhibited a reduced Cdt1 loading onto chromatin, demonstrating an exit from a G1 to S phenotype. We found HN1 and Cdh1 interaction as a new regulator of the Cyclin B1/CDK1 axis in mitotic regulation which can be explored further to dissect the roles of HN1 in the cell cycle.en_US
dc.identifier.citationJaved, A., Özduman, G., Varışli, L., Öztürk, B.E., Korkmaz, K.S. (2023). HN1 is enriched in the S-phase, phosphorylated in mitosis, and contributes to Cyclin B1 degradation in prostate cancer cells. Biology. 12(2), 189. https://doi.org/10.3390/biology12020189en_US
dc.identifier.doi10.3390/biology12020189
dc.identifier.issn2079-7737
dc.identifier.issue2en_US
dc.identifier.startpage189en_US
dc.identifier.urihttps://www.mdpi.com/2079-7737/12/2/189
dc.identifier.urihttps://hdl.handle.net/11468/11226
dc.identifier.volume12en_US
dc.institutionauthorVarışli, Lokman
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofBiology
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHN1en_US
dc.subjectCell cycleen_US
dc.subjectMitosisen_US
dc.subjectKinasesen_US
dc.subjectProstate canceren_US
dc.subjectStable cell lineen_US
dc.titleHN1 is enriched in the S-phase, phosphorylated in mitosis, and contributes to Cyclin B1 degradation in prostate cancer cellsen_US
dc.titleHN1 is enriched in the S-phase, phosphorylated in mitosis, and contributes to Cyclin B1 degradation in prostate cancer cells
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
biology-12-00189.pdf
Boyut:
1.23 MB
Biçim:
Adobe Portable Document Format
Açıklama:
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Moleküler Biyoloji ve Genetik Bölümü Koleksiyonu