Decreased expression of HN1 sensitizes prostate cancer cells to apoptosis induced by Docetaxel and 2-Methoxyestradiol

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dc.authorid0000-0002-4337-9327en_US
dc.contributor.authorVarışli, Lokman
dc.date.accessioned2022-09-02T08:04:36Z
dc.date.available2022-09-02T08:04:36Z
dc.date.issued2022en_US
dc.departmentDicle Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractObjective: Prostate cancer is one of the most frequently diagnosed cancer in men and ranks as the second most common cause of cancer-related deaths in developed countries. HN1 is a highly expressed gene in prostate cancer and controls the levels of several cell cycle regulatory proteins including Cyclin B1. Cyclin B1 is a cell cycle control protein but is also involved in Docetaxel and 2-Methoxyestradiol induced apoptosis. Since Cyclin B1 level may affect chemotherapy success and HN1-Cyclin B1 negative correlation has already been shown, so in this study, we investigated the possible role of HN1 in chemotherapeutic resistance in prostate cancer cells. Methods: Androgen-dependent and independent prostate cancer cells were used in the study. A full-length human HN1 cDNA fragment was cloned to a mammalian expression vector and this construct was used for overexpression experiments. A siRNA that specifically targets HN1 was used for HN1 depletion experiments. Evaluation of apoptosis was performed by the level of PARP cleavage and an apoptosis kit that measure Caspase 3 activity. Results: The HN1 protein level is decreased in the Docetaxel or 2-Methoxyestradiol treated LNCaP and PC-3 PCa cells whereas the Cyclin B1 level is increased. HN1 overexpression inhibited Docetaxel and 2-Methoxyestradiol induced apoptosis. In concordance, its depletion further stimulated apoptosis in drug-treated cells. However, silencing of Cyclin B1 along with HN1 abolished the increased apoptotic response induced by silencing of HN1 in Docetaxel or 2-Methoxyestradiol treated cells. Conclusion: HN1 is an anti-apoptotic molecule and inhibits Docetaxel and 2-Methoxyestradiol induced apoptosis by targeting Cyclin B1.en_US
dc.identifier.citationVarışli, L. (2022). Decreased expression of HN1 sensitizes prostate cancer cells to apoptosis induced by Docetaxel and 2-Methoxyestradiol, Annals of Clinical & Laboratory Science, 52(2), 1-6.en_US
dc.identifier.endpage6en_US
dc.identifier.issn0091-7370
dc.identifier.issn1550-8080
dc.identifier.issue2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://hdl.handle.net/11468/10315
dc.identifier.volume52en_US
dc.institutionauthorVarışli, Lokman
dc.language.isoenen_US
dc.publisherAssociation of Clinical Scientists, Inc.en_US
dc.relation.ispartofAnnals of Clinical & Laboratory Science
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergien_US
dc.relation.tubitak113S290
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.subject2-Methoxyestradiolen_US
dc.subjectApoptosisen_US
dc.subjectCyclin B1en_US
dc.subjectDocetaxelen_US
dc.subjectHN1en_US
dc.titleDecreased expression of HN1 sensitizes prostate cancer cells to apoptosis induced by Docetaxel and 2-Methoxyestradiolen_US
dc.titleDecreased expression of HN1 sensitizes prostate cancer cells to apoptosis induced by Docetaxel and 2-Methoxyestradiol
dc.typeArticleen_US

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