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Öğe Adsorption of heavy metal ions by poplar sawdust(2007) Bulut Y.; Yokus B.Decontamination of synthetic Ni(II), Cd(II) and Pb(II) solutions using poplar sawdust was investigated. Batch sorption studies have been carried out to determine the effect of contact time, temperature and initial concentration of heavy metal ions (HMI) on adsorption. Equilibrium time was found to be around 60 min. Pseudo-first-order, pseudo-second-order and intraparticle diffusion models were used to fit the experimental data. Adsorption isotherms showed that HMI adsorption on sawdust deviated from the Freundlich and Langmuir equations. The Freundlich and Langmuir constants were calculated at different temperatures (25, 45 and 60 °C). Thermodynamic parameters, such as free energy, entropy and enthalpy changes for the adsorption of heavy metal ions have been also computed and discussed. In addition, the adsorption competition of Ni(II), Cd(II) and Pb(II) ions was determined from the A% values obtained from double and triple mixtures of these ions. Ion exchange is probably one of the major adsorption mechanisms for binding divalent metal ions to poplar sawdust.Öğe Blocade of the ?-adrenoceptors with carazolol improves fertility of artificial insemination in Awassi sheep(2007) Bademkiran S.; Celik R.; Gurbulak K.; Yokus B.; Ozdemir N.This investigation aimed to study the effect on fertility parameters of one or two artificial inseminations (AI) and the administration (carazalol) before insemination of ?-adrenoceptor blocker in Awassi sheep. A total of 144 Awassi sheep with their oestrous synchronized were divided into 4 groups. Group A1 had a single artificial insemination applied at 52 hours after (the sponge was removed), group A2 had a single artifical insemination applied while for preinsemination 0.01 mg/kg carazalol were given i.v. Group B1 had artificial insemination appled twice at 52 and 60 hours. B2 group had artificial insemination appled twice at 52 and 60 hours and for preinsemination 0.01 mg/kg carazalol were given i.v. As a results, the ratios of not observed oestrus after 25 days were 55.6%, 61.1%, 63.9% and 69.4% in groups A1, A2, B1 and B 2 respectively. The ratios of pregnancy were diagnosed ultrasonographically after the 30th day of artificial insemination as 47.2%, 50.0%, 52.8% and 61.1% in groups A1, A2, B 1, and B2 respectively. The ratios of birth by means of artificial insemination were 41.7%, 47.2%, 47.2% and 55% in groups A 1, A2, B1 and B2 respectively. The degree of fertility was found to be higher in the group in which the sheep applied artificial insemination twice with 8 hours intervals and administrated ?-adrenoceptor blocker preinsemination. However, the difference between groups was not significant (p>0.05).Öğe Clinical and radiographic comparison by analyzed cone beam CT between one stage and two stage dental implants(University of Dicle, 2017) Yildirm T.T.; Kaya F.A.; Yokus B.; Colak M.; Ozdemir E.; Tekin G.G.; Saribas E.The aim of this study is to evaluate the relationship between the periodontal clinical parameters and alveolar bone loss rate (ABLR) values in one and two stage dental implants using dental tomography A total of 40 dental implant was applied to patients, 20 a one-stage surgical procedure (group I) other 20 two-stage surgical procedure (group II), who had a single missing tooth in the lower jawbone. All clinical measurements were recorded at before loading (T0), after loading at the 3 rd month (T1) and after loading at the 6 th month (T2). Alveolar bone loss rate (ABLR) values were evaluated at T0 and T3 using dental tomography because of minimal artifacts and distortions. In our study, PPD was found to be significantly high in group I than group II at T1 and T2. There was a significant relation between the mPI and ABLR at T2 in Group I, also KSTI and ABLR at T0 in Group II. The data of this study confirms that; one stage surgery procedure PPD is high than two stage surgery procedure. Larger scale studies, particularly in peri-implantitis cases, may shed more light on this subject.Öğe Comparison of Pelargonium sidoides, placebo and antibiotic treatment of chronic endometritis in dairy cows: A field trial(2009) Bademkiran S.; Kurt D.; Yokus B.; Celik R.We compared the effects of the intrauterine application of an extract of Pelargonium sidoides (EPs 7630) to parenteral antibiotic injection in the treatment of chronic endometritis, which is commonly encountered postpartum in cows. Forty six cows diagnosed with chronic endometritis 21-27 days postpartum were selected and divided into 3 groups. Cows in Group I (n = 16) received a physiological parenteral injection of 10 mL of serum per day for 3 days. Similarly, cows in Group II (n = 15) received 1 mg kg-1 ceftiofur (Ceftivil®) parenterally. Those in Group III (n = 15) received an 8 mg (10 mL)/cow intrauterine dose of EPs 7630 (Umca®) for 3 days. When examined between the 35th and 41st day, clinical recovery rates were found to be as follows: for Group 1,1/16 (6.2%); for Group II, 8/15 (53.3%) and for Group II, 4/15 (26.69%). After this examination, cows whose chronic entrometritis symptoms persisted according to clinical and rectal examination received a further 3 days of the treatment appropriate to their group. Later on various parameters were identified thus, for Groups I-III, respectively: insemination rate 87.5, 66.6 and 93.3%; days to 1st service 103.5, 91.0 and 96.0; 1st service conception rate 28.5, 40.0 and 35.7%; days open 140,120 and l31 and cows pregnant within 250 DIM 56.25, 80.0 and 66.6%. © Medwell Journals, 2009.Öğe Effects of gallic acid on ischemia-reperfusion induced testicular injury in a rat testicular torsion model(Science Printers and Publishers Inc., 2021) Sogiitcii N.; Yokus B.Objective: Testicular torsion is a common urological complication mostly affecting adolescents and young men. In this study we investigated biochemical and immunohistochemical effects of gallic acid on the damage induced by testicular torsion-detorsion. Study Design: Forty male rats were divided into 4 groups of 10 animals each: control, torsion, torsion/detorsion, and torsion/detorsion+gallic acid. Testicles were removed from the scrotum, and 2.5-hour ischemia was applied to the left testis by twisting the spermatic cord 720° clockwise. Then 3-hour reperfusion was allowed for detorsion. Gallic acid 20 mg/kg was orally administered to the torsion/detorsion+gallic acid group before reperfusion. Biochemical parameters of testicular tissue (MDA, SOD, CAT, and GSH levels) were measured. Testicular tissues were immune-stained with caspase-3 and TNF-a antibody. Results: MDA levels in the torsion/detorsion+gallic acid group were close to those in the control group; however, it was higher in the torsion/detorsion group as compared to the control group. Compared to control group, SOD, CAT, and GSH activities were significantly increased in the torsion/detorsion+gallic acid group. However, those values were decreased in the torsion-detorsion group. Spermatogenic cells and interstitial cells showed positive caspase-3 expression in the torsion and torsion-detorsion groups; however, expression level was decreased in the torsion/detorsion+gallic acid group. TNF-a expression was observed in degenerated spermatogonia, Leydig cells, and macrophages in the torsion and torsion-detorsion groups. In the torsion/detorsion+gallic acid group, TNF-a expression was observed in some interstitial region rather than in cells of seminiferous tubules. Conclusion: Gallic acid treatment could be an alternative therapy in testicular ischemia-reperfusion injury to decrease inflammation, germ cell degeneration, and apoptosis. © The Author(s), 2021.Öğe Effects of lycopene and vitamine E administration over gastric mucosal damage induced by Aflatoxin B1(2009) Kurt D.; Saruhan B.G.; Yokus B.; Cakir D.U.In the present study, we aimed to determine, the changes induced by Aflatoxin B1 (AFBj) administration on rat gastric mucosal barrier and gastric mucins alongside revealing the protective effects of lycopene and Vit E. Thirty-five Wistar-Albino male rats weighing 180-220 g, were divided into 7 groups as to include 5 rats in each group: control, lycopene (10 mg/kg/day lycopene for 15 days (Lycopene 10% FS), AFB, (single dose of 2.5 mg AFBj kg-1 on the 12th day of the study), Lycopene + AFB1 (10 mg lycopene/kg/day for 15 days and single dose of 2.5 mg AFB1 kg-1 on the 12th day of the study) and Vitamin E + AFB1, (10 mg/kg/day Vitamin E for 15 days and single dose 2.5 mg AFB1, kg-1 on 12th day). Following the sacrifice of study subjects on the 15th day, gastric mucus and phospholipid levels were determined and their stomachs were examined histopathologically. Examination of mucus and phospholipid levels revealed a significant reduction in group 3-5, in which AFB1 has been applied (respectively, p<0.001, p<0.001). When lycopene and vitamine E groups are compared with the AFB1 group, a significant elevation was detected in mucus and phospholipid levels (respectively, p<0.001, p<0.001). Whereas, histopafhological examination of gastric mucosas of the aflatoxin group showed degenerative changes, gastric mucosas of the control group and the remaining study groups were normal. Histochemically, while neutral mucins were predominant in general structure of stomach, mixed and sialomucins were observed, as well. Particularly acid mucins with suplhate and periodate reactive acid mucins were found to be more predominant in the aflatoxin group compared to control and other groups. Histochemical features of mucins were observed to be consistent with specific functions of the different regions of stomach. Lycopene and vitamine E administrations were found to be protective against the damage induced by aflatoxin on gastric mucosa. © Medwell Journals, 2009.Öğe Significance of changes in lipid peroxides and antioxidant enzyme activities in pregnant women with preeclampsia and eclampsia(2000) Bayhan G.; Atamer Y.; Atamer A.; Yokus B.; Baylan Y.This review addresses the general hypothesis that the pathogenesis of preeclampsia and enclampsia are related to an imbalance of increased oxidative stress and lipid peroxidation coupled with a deficiency of antioxidant protection. Accordingly, this study was initiated to assess total antioxidant status and free-radical activity in preeclampsia and eclampsia. The patients studied were 44 healthy pregnant women and 45 women with hypertension classified as having preeclampsia (n = 27), and eclampsia (n = 18). The serum levels of lipid peroxide were significantly increased (p < 0.0001) and antioxidant enzyme activities (superoxide dismutase and gluthathione levels) in erythrocytes were significantly decreased (p < 0.0001) in women with preeclampsia and eclampsia compared with the controls. The groups of preeclampsia and eclampsia had similar values of catalase activities as the controls (p > 0.05). There were no correlations between serum levels of lipid peroxide and antioxidant enzyme activities or systolic-diastolic blood pressure of pregnant women with preeclampsia and eclampsia. The mean systolic and diastolic blood pressure, the serum lactate dehydrogenase (LDH) and aspartate transaminase (AST) levels of preeclamptic and eclamptic women were high, whereas haemoglobin (Hb), Hematocrit (Htc) and platelet levels were lower than those of the control subjects (p < 0.0001). There were no differences in mean gestational week, whereas the mean age of eclamptic women was lower than that of the other two groups (p < 0.001). The serum levels of Alanin-transaminasis (ALT) and urea in eclamptic women were significantly higher compared with the other two groups (p < 0.0001), whereas creatinin levels were lower than those of the other two groups (p < 0.05). Our findings give support to those few studies considering lipid peroxidation as an important factor in the pathogenesis of preeclampsia and eclampsia. Further studies are needed to clarify the relations between lipid peroxidation and antioxidative function and their pathophysiological significance in preeclampsia and eclampsia.Öğe Time-dependent changes in plasma ghrelin and angiotensin II levels in the diabetic nephropathy model(Parlar Scientific Publications, 2021) Gul C.; Kelle M.; Baylan M.; Yokus B.; Tasdemir E.Ghrelin is an appetite-enhancing anabolic hormone secreted from the stomach. Angiotensin II maintains sodium and potassium levels in body fluids and plays a very important role in the regulation of arterial blood pressure. Although their relationship with Type 2 diabetes and complications have been reported, their role in diabetic nephropathy is not fully understood. We investigated time dependent possible changes in plasma ghrelin and angiotensin II levels during the development and progression of diabetic nephropathy in experimental diabetic rat model. Adult 63 male Wistar Albino rats were randomly divided into 9 groups as 4 control (C1-C4), 4 diabetic (D1-D4) and one treatment (T) group. Group D1, sacrificed by cardiac puncture one week after diabetes, group D2 three weeks later, group D3 six weeks later, and groups D4 and T eight weeks later. Antidiabetic treatment was not administered to the D1-D4 group diabetic rats. Group T diabetic rats were treated with antidiabetic metformin (100 mg / kg / day) for 8 weeks. A single dose of 35 mg / kg intraperitoneal streptozotocin was administered to the rats to induce diabetes. Significant differences were found between the D4 and C4 groups in body weight, plasma glucose, ghrelin and angiotensin II, serum and urine creatinine levels. While there was a linear (positive) relationship between plasma ghrelin levels of all rats and urinary creatinine and creatinine clearance and body weight, negative correlations were found between plasma ghrelin and angiotensin II levels and fasting blood glucose levels of all rats. During the progression of diabetes and the development of diabetes-related nephropathy, plasma angiotensin and serum creatinine levels increased, while plasma ghrelin levels decreased over time. Therefore, it was concluded that changes in plasma ghrelin and angiotensin II levels in diabetic rats may be associated with the pathogenesis of diabetic nephropathy. © 2021 Parlar Scientific Publications. All rights reserved.Öğe Total anti-oxidant capacity and oxidative stress in dairy cattle and their associations with dystocia(2007) Yokus B.; Bademkiran S.; Cakir D.U.The aim of study was to investigate pre-partum and postpartum changes in the total anti-oxidant capacity (TAC), total peroxide (TPX) and oxidative stress index (OSI) of cows with dystocia compared with healthy animals. The possible relationships between these markers and relevant blood parameters were also investigated. Examinations were performed in a herd of 200 Holstein cows, aged 3-6 years. Blood samples were taken both during the 7th month of pregnancy and within the first 15 minutes postpartum. After calving, the cows were divided into two groups according to the type of birth: Group 1 dystocia (n=16) and (Group 2) normal partum (n=21). Additionally, cows in the dystocia group were divided into three subgroups, according to the reason for the dystocia: absolute birth weight (the high birth weight of calf), twin pregnancy and presentation. The results indicated that there were no changes in oxidative stress either in dystocia or during normal parturition eutoci. Oxidative stress may be decreased using an antioxidant supplement during the pre-partum period. The study also showed that oxidative stress does not affect dystocia. It may also be concluded that additional antioxidant supplementation may be required in absolute birth weight and twin pregnancy to reduce TPX levels. Analyses of urea, uric acid, and creatinine, as well as the enzyme activities of lactate dehydrogenase, alanine and aspartate transferase pre-partum do not appear to be useful for predicting dystocia. However, decreasing serum total protein may be a parameter to be used in diagnosing pathological conditions in pregnancy - particularly during late pregnancy.
