Yazar "Yavuz, Dilek" seçeneğine göre listele
Listeleniyor 1 - 7 / 7
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Effects of thymoquinone on spinal cord injury in rats(Pisa Univ Press, 2022) Avinca, Oner; Tas, Mahmut; Karakoc, Yenal; Yavuz, Dilek; Deveci, EnginPurpose: Spinal cord injury (SCI) is a condition that causes disturbances in normal sensory, motor, and autonomic functions. During SCI, damages occur such as, contusion, compression, distraction. The aim of this study was to investigate effects of the antioxidative thymoquinone on neuron and glia cells in SCI biochemically, immunohistochemically and ultrastructurally. Methods: Male Sprague-Dawley rats were divided into Control, SCI and SCI + Thymoquinone groups. After T10 -T11 laminectomy was performed, a metal weight of 15 grams was left down the spinal tube for spinal damage. Immediately after the trauma, the muscles and skin incision were sutured. Thymoquinone was given to the rats by gavage as 30mg/kg/21days. Tissues fixed in 10% formaldehyde, embedded in paraffin wax and immunstained with Caspase-9 and phosphorylated signal transducer and activator of transcription 3 (pSTAT-3) antibodies. Remaining were stored at-80oC for biochemistry. Frozen spinal cord tissues were placed in a phosphate buffer solution and homogenized, centrifuged then used to measure malondialdehyde (MDA) levels, glutathione peroxidase (GSH) and Myeloperoxidase (MPO).Results: In the SCI group, MDA, MPO, neuronal degeneration, vascular dilatation, inflammation, apoptotic appearance in the nucleus, loss of membrane and cristae in mitochondria, and dilatation in the endoplasmic reticulum were observed due to degeneration in the neuron structure. In the electron microscopic examination of the trauma + thymoquinone group, the membranes of the nuclei of the glial cells were thick and euchromatin, and mitochondria were shortened in length. In the SCI group, pyknosis and apoptotic changes were observed in neuronal structures and nuclei of glia cells in the substantia grisea and substantia alba region, along with positive Caspase-9 activity. An increase in Caspase-9 activity was observed in endothelial cells in blood vessels. In the SCI + thymoquinone group, Caspase-9 expression was positive in some of the cells in the ependymal canal while the cuboidal cells showed a negative Caspase-9 reaction in the majority. A few degenerated neurons in the substantia grisea region showed a positive reaction with Caspase-9. In SCI group, pSTAT-3 expression was positive in degenerated ependymal cells, neuronal structures, and glia cells. pSTAT-3 expression was positive in the endothelium and surrounding aggregated cells of the enlarged blood vessels. In the SCI+ thymoquinone group, pSTAT-3 expression was negative in most of the bipolar and multipolar neuron structures and glial cells in ependymal cells, enlarged blood vessel endothelial cells.Conclusions: It has been thought that thymoquinone application in spinal cord injuries may be an antioxidant that can be recommended as an alternative treatment in suppressing the apoptosis of neural cells by significantly reducing the inflammation process.Öğe Immunohistochemical and Histopathological Changes in the Skin of Rats After Maneb Application(Sci Printers & Publ Inc, 2014) Sula, Bilal; Ekinci, Cenap; Ucak, Haydar; Ucmak, Derya; Akkurt, Zeynep Meltem; Yavuz, Dilek; Arica, MustafaOBJECTIVE: To determine the immunohistochemical and histo pathological changes in facial skin after exposure to maneb (manganese ethylene bisdithiocarbamate), a fungicidal dithiocarbamate pesticide. STUDY DESIGN: In the experimental group maneb was administered by inhalation to 10 male Wistar albino rats for 5 days each week for 3 weeks. As a biological control, the control group (n=10) received distilled water by spray for the same time period. The experiment was terminated after 3 weeks. Sections of rat facial skin were examined histopathologically. RESULTS: In the experimental group, microscopic examination of facial skin revealed degeneration of the epidermis, detection of mild inflammatory reaction, and vascular dilation in the connective tissue. Hair follicles and degenerative changes were observed in the deeper parts. In the experimental group, dilation of the blood vessels in the dermis and hemorrhage were supported by an increase in CD34 expression. In addition, a reduction in the number of melanocytes (hypopigmentation) was observed in the hair follicles and epidermis, along with a decrease in the expression of CD117. CONCLUSION: Epidermal degeneration, intradermal cell infiltration, vascular changes, and reduction in the number of melanocytes in the follicle and content of cytokeratin in both the epidermis and hair follicle ker-atinocytes were detected after maneb application. These findings may have important implications in the association with main signaling pathways, including keratinocyte proliferation and differentiation. Disruption of these pathways may cause some dermatoses.Öğe Investigation of Blood Natural Killer Cell Activity in Normozoospermic Fertile, Oligozoospermic, Severe-Oligozoospermic, Oligoastenozoospermic, Azoospermic and Idiopathic Individuals(Mardin Artuklu University, 2023) Özmen, M. Ferit; Afşin, Muhamet; Şeker, Uğur; Yavuz, Dilek; Bademkıran, Muhammed Hanifi; Cirit, Ümüt; Yıldırım, İbrahimIntroduction: Infertility is a health problem that negatively affects couples and society in many ways and is becoming increasingly common. It is estimated that 30-40% of these problems are caused by men. It was aimed to determine whether natural killer (Natural Killer: NK) cell activity changes in male individuals with different degrees of infertility problems and in individuals with normal sperm count and concentration (normozoospermia). Methods: NK cell activities were measured from blood samples taken from individuals who were determined to be oligozoospermia, severe-oligozoospermia, oligoastenoozospermia, azoospermia and idiopathic as a result of semen analysis with from individuals diagnosed normozoospermia (n:120). Results: NK cell activity in blood was observed as the lowest in normozoospermia (544.46 pg/ml), but the highest value measured in severe oligozoospermia group (1005.90 pg/ml). On the other hand, NK cell activity was measured 797.60 ± 428.55 pg/ml, 905.34 ± 430.60 pg/ml, 757.66 ± 541.16 pg/ml and 639.44 ± 385.50 pg/ml in oligozoospermia, oligoastenozoospermia, azoospermia and idiopathic groups respectively. While the difference in NK activity between the severe oligozospermia group and the other groups was significant (p0.05). Conclusion: It was determined that NK cell activity was higher in individuals with severe oligozoospermia than normozoospermia, oligozoospermia, oligoastenozoospermia, azoospermia and idiopathic individuals.Öğe Investigation of FAS and IL-6 Expression in Placentas with HELLP Syndrome(Soc Chilena Anatomia, 2022) Otcu, Serap; Atic, Esra Nur; Yavuz, DilekThe aim of our study was to investigate the effect of inflammation in the placenta on the pro-apoptotic development after severe preeclampsia. Placenta tissue samples of 15 HELLP syndrome and 15 healthy 35-38th week-pregnant women were involved in the study. Tissue samples were taken only from the maternal side of the placenta and fixed in 10 % formaldehyde, then blocked in paraffin wax and 4-6 mm-thick sections were cut and stained with Harris Hematoxylcne-Eosin. Antigen retrieval was performed for sections, incubated with FAS antibody and anti-IL-6 antibody. After the application of streptavidin peroxidase followed by AEC chromogen solution, sections were cotmterstained with I laths hematoxylin. Significant thickening of the fibrinoid layer, degeneration and apoptotic change in decidua cells, marked increase in the hyalinized area, degenerative changes in the syncytial regions of the chorionic villus and an increase in syncytial nodes and bridges and IL- expression were observed as positive. FAS expression was positive in the pycnotic nuclei of decidual cells in the maternal region and in the syncytial regions. It was observed that the proapoptotic process increased as a result of severe preeclampsia. It was concluded that the control of cytokine activity and reduction of pro-apoptotic signal during the inflammation process will slow down the development of HELLP syndrome.Öğe Sağlıklı, preeklampsi ve hellp vakalarında plasental sinsityal düğümlerin histolojik yönden karşılaştırılması(2015) Yavuz, DilekÖZET Preeklampsi, perinatal ölümlerin ve sakatlıkların ana nedenlerinden biri olup gebede maternal hipertansiyon, proteinüri, artmış damar hasarı ve geçirgenliği ile karakterize olan multisistemik bir bozukluktur. Çalışmamızda, artan sinsityal düğümlerin ultrastrukturel düzeyde incelenecek olması, söz konusu düğümlerde olası değişikliklerin gerek preeklampsi ve de HELLP vakalarında ortaya konularak, sinsityal düğüm artışının etkisi ortaya konacaktır. Örnek plasenta ve plasenta yataklarının morfolojik ve ultrastrüktürel değisimlerini gözlemlemek için ışık ve elektron mikroskop tekniklerini kullandık. Doku örnekleri 10 preeklampsili, 10 HELLP’li ve 10 kontrol grubunu oluşturan sağlıklı gebeden alındı. Yapılan ışık ve elektron mikroskobi yöntemleri ile preeklampsili hastaların, HELLP’li hastaların ve kontrol grubu gebelerin sonuçları değerlendirildi. Işık ve elektron mikroskop çalışmaları sonucunda plasenta dokularında: Preeklemsi grubu fetal periferik kesitlerde; sinsityotrofoblastlarda sitoplazmik yaygın vakuolizasyonlar ve endoplazmik retikulum sarnıçlarında dilatasyon gözlenirken, kapiller endotel hücrelerinde incelme ve kapillerin temas ettiği sinsityotrofoblastlarda nekrotik görünümün hâkim olduğu gözlendi. Bu gruptaki sinsityotrofoblastlarda mikrovillus sayısının azalması yanı sıra bağ doku ödemi dikkati çeken bulgulardan biriydi. HELLP maternal periferik ve sentral kesitler incelendiğinde: her iki yapıda da sinsityotrofoblastlarda intrasitoplazmik ödem ve dejeneratif vakouller gözlenirken, ayrıca villöz ödem belirgin olarak dikkati çekmekteydi. HELLP fetal periferik kesitlerde, intervillöz alanda yoğun hücresel debris varlığı dikkati çeken en önemli bulguydu. Kontrol grubuyla kıyaslandığında preeklampsi ve HELLP’li plasentalarda sinsityal düğüm sayısında artış ve ultrastrukturel yapıda pek çok histolojik değişiklikler izlendi. Anahtar Kelimeler: Preeklampsi, HELLP, Sinsityal Düğüm, Ultrastrukturel yapı ABSTRACT Preeclampsia is one of the main causes of perinatal deaths and disabilities and is a multisystemic disorder that is characterized by maternal hypertension, proteinuria, increased permeability of blood vessel damage. In our study, the fact that increased syncytial nodes will be examined at ultrastructural level, the effect of the increase in syncytial node will be revealed by putting forward the possible changes in the mentioned nodes both in women with preeclampsia and HELLP cases We used the light and electron microscope techniques to observe morphological and ultrastructural changes in the placenta and placental deposits. Tissue samples were taken from 10 preeclamptic, 10 pregnant with HELLP and 10 healthy pregnant women from the control group. Through light and electron microscopy methods, the results of patients with preeclampsia, control group of pregnant women and patients with HELLP were evaluated. As a result of light and electron microscopy studies, in placental tissues: in Preeclampsia group, while in fetal peripheral sections; we observed cytoplasmic common vacuolisations in syncytiotrophoblasts and dilatation in endoplasmic reticulum cisterns, thinning in capillar endothelial cells and necrotic appearance in syncytiotrophoblasts in contact with capillaries were found to be the dominant view. In this group, reduction in the number of microvilli of syncytiotrophoblasts as well as the connective tissue edema was one of the most remarkable findings. When HELLP maternal peripheral and central cross-sections were analyzed, intracytoplasmic edema and degenerative vacuollar were observed in syncytiotrophoblasts in both structures, and villous edema seemed to be obvious. In HELLP fetal peripheral sections, the presence of dense cellular debris in intervillous space was the most important finding. Compared with the control group, increase was observed in the number of syncytial nodes and histological changes in ultrastructural structure in placentas with preeclampsia and HELLP, Keywords: Preeclampsia, HELLP, Syncytial knot, Ultrastructural Structure.Öğe A Single-Center Study Assessing the Relationship Between Smoking Habits and sperm Parameters in Men with Suspected Infertility(2024) Afşin, Muhamet; Nursal, Ayşe Feyda; Yavuz, Dilek; Akkoç, HasanObjective: Many studies have linked smoking to male infertility. Therefore, we aimed to investigate the effect of smoking on sperm parameters in men with suspected infertility in the Turkish population. We also examined the effect of daily smoking amount and smoking duration on sperm parameters. Material and Method: This study consisted of 1005 men (smokers= 599, non-smokers= 406) with suspected infertility. It evaluated sperm parameters, including, leucocyte count, sperm concentration, total sperm count, motility, and the total progressive motile sperm count in these men. Results: In our study group, 59.60% were smokers and 40.40% were non-smokers. Body mass index (BMI) was higher in non-smoker males. Sperm characteristics were similar in smokers and non-smokers. We evaluated the smoker's group according to the number of cigarettes smoked per day. There was no significant difference in sperm parameters between the group that smoked up to 30 cigarettes a day and the group that smoked more than 30 cigarettes. Then, we examined the smokers in 3 groups according to the duration of smoking: 0-10 years, 11-20 years, and 20 years and over. It was observed that non-progressive motility was the lowest and immotility was the highest in smokers who had been smoking for 20 years or more. Conclusion: This study is the most comprehensive study in Turkey examining the relationship between smoking and sperm parameters to the best of our knowledge. Our results show that the duration of smoking affects sperm functions. Our evidence indicates that men with suspected infertility should quit smoking to optimize their successful conception.Öğe SPERM PARAMETERS' PREDICTIVE VALUE IN INTRAUTERINE INSEMINATION SUCCESS: A SINGLE-CENTER EXPERIENCE(Bilal GÜMÜŞ, 2023) Afşin, Muhamet; Nursal, Ayşe Feyda; Otcu, Serap Mutlu Özçelik; Yavuz, Dilek; Ege, SerhatAlthough intrauterine insemination is a widely used assisted reproductive technique there is no consensus on sperm parameters that affect the probability of pregnancy. Therefore, the purpose of this study was to determine whether semen parameters affect intrauterine insemination success. A total of 403 couples (345 negative pregnancies, and 58 positive pregnancy) that underwent 549 intrauterine insemination treatment cycles for heterogenous indications were included in the study. Clomiphen citrate, letrazole and/or gonadotropins were used for ovarian stimulation in women in this study. The spermiogram tests of the spouse of receiving IUI tretment were examined. Clinical pregnancy occurred in 58 of 549 intrauterine insemination (10.56%). Unexplained infertility, polycystic ovary syndrome, and cervical-tubal factors were higher in positive pregnancy group compared to negative pregnancy group while male factor was more prevalant in negative pregnancy group comparison to positive pregnancy group (p=0.03). There was no statistical difference between women with positive and negative pregnancy in terms of age (? 35 and > 35), body mass index, infertility type, infertility time, endometrial thickness on HCG day, stimulation protocol, cycle numbers, number of insemination, estradiol, prolactin, LH, FSH, and TSH levels (p>0.05). The sperm characteristics in males [ejaculate volum, sperm concentration, total sperm count, motility, immotility, and total progressive motile sperm count] did not significantly differed between positive and negative pregnancy groups (p>0.05). Briefly, the present study found that the conception probability of intrauterine insemination did not correlate with the spermiogram parameters.
