Yazar "Ullah, Zain" seçeneğine göre listele

Listeleniyor 1 - 5 / 5
  • [ X ]
    Öğe
    Heavy Metals, Trace and Major Elements in 16 Wild Mushroom Species Determined by ICP-MS
    (Atomic Spectroscopy Press Ltd, 2018) Tel-Cayan, Gulsen; Ullah, Zain; Ozturk, Mehmet; Yabanli, Murat; Aydin, Firat; Duru, Mehmet Emin
    Eight minerals (Na, Mg, Ca, V, Mn, Fe, Zn, Se) and eight heavy metals (Al, Cr, Ni, As, Sr, Co, Cu, Pb) were studied in 16 wild mushroom species: Agarlats blsporus, Agaricus bitorquis, Agaricus essettei, Armtllaria tabescens, Craterellus cornucoptoides, Ilebeloma eburneum, Hebeloma fragilipes, Hypboloma fasciculare, Lepista nuda, Leacoagaricus leucotbites, Melanoleuca gramtnicola, Ompbalotus oleartus, Pbelltnus torulosus, Ramaria flava, Russula foetens, and Trametes ventcolor collected from Usak, Turkey, using ICP-MS. The mineral content of the mushroom samples was determined by ICP-MS and ranged from 46-1717 mg/kg for Na, 122-1121 mg/kg for Mg, 15-316 mg/kg for Ca, 0.09-1.04 mg/kg for V, 3.60-76.40 mg/kg for Mn, 17.3-395 mg/kg for Fe, 3.4-70.7 mg/kg for Zn, 0.03-0.56 mg/kg for Se. The heavy metals contents in the mushroom species were between 108-575 mg/kg for Al, 0.08-0.61 mg/kg for Cr, 0.18-3.95 mg/kg for Ni, 0.19-4.43 mg/kg for As, 2.41-14.9 mg/kg for Sr, 0.02-3.44 mg/kg for Co, 0.17-9.89 mg/kg for Cu, 0.05-3.33 mg/kg for Pb. All values of the inedible mushrooms, except the Cr and Pb concentrations of Lencoagarlcus leucothttes, were within the permitted WHO limits. Thus, the mushrooms analyzed are safe for human consumption.
  • [ X ]
    Öğe
    In vitro antioxidant, anticholinesterase, tyrosinase activity studies, and LC-MS/MS simultaneous determination of 37 bioactive compounds in Indigofera heterantha
    (Elsevier, 2022) Ullah, Zain; Ali, Saqib; Hussain, Atarad; Ozturk, Mehmet; ERTAS, Abdulselam; Alamzeb, Muhammad; Rashid, Mamoon Ur
    The flowering plant Indigofera heterantha generally known as Indigo of Himalayan, was extracted with nhexane, ethyl acetate, and methanol by using Soxhlet apparatus. The extracts were studied for their antioxidants and enzyme inhibition activities. Against DPPH free radical-scavenging activity methanol extract was found moderately active (IC50: 158.41 +/- 1.1.66 mu g/mL). The ethyl acetate and n-hexane extracts exhibited the best ABTS radical scavenging activity (IC50: 31.32 +/- 2.50 and 52.0 +/- 0.89 mu g/mL respectively). Against the metal chelating activity, n-hexane extract was active IC50: 52.36 +/- 0.95 mu g/mL. The n-hexane and ethyl acetate extracts demonstrated the best b-carotene antioxidant activity (IC50: 22.32 +/- 0.84 and 60.66 +/- 0.35 mu g/mL respectively). Against acetylcholinesterase (AChE), ethyl acetate and methanol extracts were moderate active (IC50: 133.58 +/- 0.870, and 137.97 +/- 0.97 mu g/mL respectively). The ethyl acetate extract showed Butyrylcholinesterase (BChE) inhibitory activity with IC50 value of 121.9 +/- 1.13 mu g/mL. Against tyrosinase activity, ethyl acetate and n-hexane extracts activity with an IC50 value of 79.25 +/- 0.15 and 131.48 +/- 0.88 mu g/mL respectively. The phenolic contents of the methanolic extract of I. heterantha were also determined by using LC-MS/MS. The major constituents were Quinic acid (57,333.04 mu g/g), malic acid (1135.16 mu g/g), gallic acid (201.13 mu g/g), isoquercitrin (113.63 mu g/g), Rutin (92.97 mu g/g), and Salicylic acid (40.45 mu g/g). Keeping in view the overall biological activities of I. heterantha, it might be used in food industries and pharmaceuticals as a potential functional food ingredient.(c) 2022 SAAB. Published by Elsevier B.V. All rights reserved.
  • Küçük Resim
    Öğe
    In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical fingerprints of five Moroccan seaweeds
    (Elsevier B.V., 2020) Grina, Fatiha; Ullah, Zain; Kaplaner, Erhan; Moujahid, Abderrahman; Eddoha, Rabiaa; Nasser, Boubker; Ertaş, Abdulselam; Öztürk, Mehmet
    The coastlines of Morocco have an abundant resource of diverse seaweeds but the bioactivity of many of these seaweeds is still unexplored. The aim of this study was to contribute to the evaluation of the antioxidant potential, using different in vitro systems and searching some fingerprints of phenolic constituents based on LC-MS/MS method of five seaweeds species obtained from Moroccan Atlantic Ocean: Bifurcaria bifurcata, Cystoseira humilis, Cystoseira stricta, Fucus spiralis and Gelidium sesquipedale. The total phenolic compounds, the antioxidant activity, anticholinesterase, tyrosinase inhibitory, urease inhibitory activities and the organic compounds were determined. The results showed that Fucus spiralis possessed the highest phenolic and flavonoid contents compared to the others. In addition, Fucus spiralis (IC50: 13.25 ± 0.9 µg/mL) and Cystoseira stricta (IC50: 13.58 ± 1.2 µg/mL) exhibited higher lipid peroxidation inhibitory activity. The highest1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50: 47.23 ± 3.8 µg/mL), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) dia-mmonium salt (ABTS) (IC50: 5.95 ± 0.6 µg/mL), and Cupric reducing antioxidant capacity (CUPRAC) (55.24 ± 0.04 µg trolox equivalents/mg) activity was measured in Fucus spiralis. Regarding metal chelating activity, Bifurcaria bifurcata was the best (IC50: 45.22 ± 0.9 µg/mL) compared to the others. The highest acetylcholinesterase (IC50: 28.92 ± 1.8 µg/mL), butyrylcholinesterase (IC50: 11.72 ± 2.8 µg/mL), tyrosinase (IC50: 6.19 ± 0.4 µg/mL) and urease (IC50: 10.96 ± 1.3 µg/mL) inhibitory activities was found in Fucus spiralis. According to LC-MS/MS results, quinic acid, malic acid, fumaric acid, gallic acid, protocatechuic acid, 4-OH-benzoic acid, hesperidin, were detected in ethanol extracts of the five Moroccan seaweeds. This study demonstrated that seaweeds, particularly Fucus spiralis and Cystoseira stricta could be a promising source of antioxidants and phytochemicals which can be used in cosmetics, pharmaceutical and as nutritional food supplement in food industries to deleterious free radical induced stress, disorder and diseases.
  • Küçük Resim
    Öğe
    Insight into isolation and elucidation of cytotoxic ergostanoids from the mushroom Sarcosphaera crassa (Santi) Pouzar: An edible mushroom
    (Elsevier Science INC., 2022) Ullah, Zain; Öztürk, Mehmet; Ertaş, Abdulselam; Wahab, Atia-tul; Ben Mansour, Riadh; Choudhry, M. Iqbal
    Sarcosphaera crassa is a mushroom consumed in Europe and Anatolia after being cooked well. The cytotoxic activity of the extracts of unbaked S. crassa against MCF7, HT29, HeLa cancer cell lines and toxicity against PDF fibroblast healthy cell lines were studied using MTT assay. Acetone and methanol extracts of the mushroom exhibited significant cytotoxic activity. Further investigation of cytotoxic extracts afforded two new fatty acid sterols (1-2), a new ergosterol glycoside (4), and seven known compounds, including a fatty acid sterol (3), a steroid glycoside (5), two ergostanoids (6-7) and three sugars (8-10). These compounds were identified as brassicasteryl heptadecanoate (1), brassicasteryl palmitoleate (2), brassicasteryl linoleate (3), brassicasterol beta-?-xylofuranoside (4), brassicasterol beta-?-glucoside (5), brassicasterol (6), ergosterol-endoperoxide (7), mannitol (8), erythritol (9) and turanose (10). Among them, 7 exhibited a moderate cytotoxic activity against HeLa (IC50: 70.1 +/-& nbsp; 2.0 mu g/mL) and high activity against HT29 (IC50: 38.8 +/- 0.9 mu g/mL), and MCF7 (IC50: 62.9 +/- 1.3 mu g/mL) cancer cell lines. Compounds 4, 5, and 6 also exhibited significant cytotoxic activity against HT29 and MCF7. Moreover, all compounds exhibited weak toxicity against PDF healthy cell lines. This study indicates the potential use of Sarcosphaera crassa as a natural source of cytotoxic ergostanoids, which can be considered a dietary supplement for cancer prevention.& nbsp;
  • Küçük Resim
    Öğe
    Lc-ms/ms simultaneous determination of 37 bioactive compounds in bunium crassifolium batt. And its biological activities
    (Marmara University, 2021) Souilah, Nabila; Bendif, Hamdi; Ullah, Zain; Hamel, Tarek; Djarri, Lakhdar; Öztürk, Mehmet; Ertaş, Abdulselam
    Bunium crassifolium Batt. is an extremely rare species growing in Algeria. Its leaf or aerial part is used for flavoring and garnishing food. The aim of the current study was to determine 37 bioactive phytochemicals and biological evaluation of the methanol and methanol:water (70:30) extracts of B. crassifolium for the first time. A total of 10 phenolic acids and 8 flavonoids have been identified in the extracts of the aerial parts by LC–MS/MS. Chlorogenic, gallic and ferulic acids were the most abundant phenolic acids detected, while rhoifolin, quercitrin and rutin were the most abundant flavonoids. In addition, 3 non-phenolic organic acids (fumaric acids, quinic and malic) were detected and among them quinic and malic acids were the most abundant. Antioxidant activity was evaluted by six methods and the extracts showed significant activity. The total phenolic and flavonoid contents were determined and methanol:water (70:30) extract showed higher values which entails its greater antioxidant capacity. In addition, anticholinesterase (AChE and BChE) activity was evaluated for both extracts using Ellman method. Methanol extract showed a better anti-acetylcholinesterase activity than methanol:water (70:30) one. While, both extracts showed a weak anti-butyrylcholinesterase activity. Furthermore, the anti-tyrosinase activity was tested and the methanol:water (70:30) extract was promising and more potent. In conclusion, B. crassifolium could be used in food industries and pharmaceutical as a potential functional food ingredient.