Yazar "Tilkat, E" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    In vitro propagation of khinjuk pistachio (Pistacia khinjuk stocks) through seedling apical shoot tip culture
    (Sejani Publ, 2005) Tilkat, E; Isikalan, C; Onay, A
    An efficient method was developed for in vitro propagation of Pistacia khinjuk (khinjuk pistachio). Shoot cultures were established from aseptically germinated seedlings of khinjuk pistachio. Murashige and Skoog (MS) medium with Gamborg's vitamins containing 30 g P sucrose, 100 mg l(-1) I-ascorbic acid, 1 mg l(-1) BA and 7 g l(-1) agar resulted in multiple shoot initiation at the rate of 7.25 +/- 0.95 shoots per explant in 28 days of culture. Efficient rooting was achieved in MS medium supplemented with 0.5 mg l(-1) IBA. The in vitro raised plants were acclimatized in a growth room and transplanted to the field successfully. The method described will be useful for rapid multiplication of khinjuk pistachio for commercial exploitation.
  • [ X ]
    Öğe
    Somatic embryogenesis of pistachio from female flowers
    (Taylor & Francis Ltd, 2004) Onay, A; Pirinç, V; Tilkat, E; Aktürk, Z; Yildirim, H
    Induction of somatic embryogenesis from the flower buds of pistachio (Pistacia vera L.) was studied with respect to growth regulators. Sixty-five percent of cultures formed embryogenic calli when placed on modified Murashige and Skoog medium containing 1 mg l(-1) benzylaminopurine. In contrast, thidiazuron and a-naphthalene acetic acid reduced embryogenesis. Some of the somatic embryos had fused hypocotyls or multiple cotyledons. Abscisic acid was not necessary for maturation. Fewer somatic embryos germinated with 0.5 mg l(-1) abscisic acid, but more converted into plantlets. Separate, morphologically normal, somatic embryos germinated on semi-solid germination medium, and developed into plantlets. Cytological analysis revealed a chromosome number of 2n = 2x = 30, similar to seedlings. The protocol described in this paper, for the induction of somatic embryos indirectly from female flowers of pistachio, represents a first step towards the production of clonal stocks.