Yazar "Tekin A." seçeneğine göre listele

Listeleniyor 1 - 11 / 11
  • [ X ]
    Öğe
    [Acinetobacter baumannii: a rare cause of deep neck infection]
    (2012) Kınış V.; Bakır S.; Ozbay M.; Yorgancılar E.; Topçu I.; Tekin A.
    [No abstract available]
  • [ X ]
    Öğe
    Anti-tuberculosis drug resistance in southeast of turkey
    (Acta Medica Mediterranea, 2013) Dal T.; Özcan N.; Tekin R.; Tekin A.; Çelen M.K.; Özekinci T.
    Objective: Tuberculosis is a globally prevalent life-threatening infectious disease. In this study we aimed to evaluate antibiotic suscebtibility rates of Mycobacterium tuberculosis complex strains. Materials and methods: A total of 150 culture-positive samples were included in. Among culture positive samples 86 were Mycobacterium tuberculosis complex and 64 were non-tuberculous mycobacterium. Of Mycobacterium tuberculosis complex isolated samples 45 were Ziehl-Neelsen positive. Of 64 non-tuberculous mycobacterium isolated samples 15 were Ziehl-Neelsen positive. Mean age of the patients with tuberculosis was 37.74±20.53 and non-tuberculous mycobacterium isolated patients was 46.80±23.32. Antibiotic susceptibility testing was performed for the four first-line anti-tuberculosis drugs by BACTEC MGIT-960 instrument (Becton Dickinson). Of M. tuberculosis strains 41 % was resistant to at least one or more of the drugs (isoniasid 27.9%, ethambutol 8.13%, streptomycin 12.79 %, rifampicine 9.3 %). Of the strains 5.8 % was resistant to two, 3.4 % to three and 3.4 % to four drugs. Conclusion: Mycobacterium tuberculosis complex and drug resistance of this microorganism continued to be a problem for our country and non-tuberculous mycobacterium species may become a problem in the future. We thought that a strong and costeffective tuberculosis control programme contributes to reduce the incidence of drug resistance in the community.
  • [ X ]
    Öğe
    Assessment of methicillin and clindamycin resistance patterns in staphylococcus aureus isolated from a tertiary hospital in Turkey
    (2013) Tekin A.; Dal T.; Deveci O.; Tekin R.; Atmaca S.; Dayan S.
    In order to detect the methicillin susceptibility and determine the prevalence of constitutive and inducible clindamycin resistance in Staphylococcus aureus strains, a total of 254 S. aureus isolates, including 139 (54.7%) methicillin-sensitive and 115 (45.3%) methicillin-resistant, were tested for clindamycin and erythromycin by Kirby-Bauer's disk diffusion method. The disk diffusion induction test or D-test was performed on erythromycin-resistant and clindamycin-sensitive strains using the disk diffusion method. The erythromycin-resistant and clindamycin-sensitive strains with a D-shaped zone around the clindamycin disk were considered positive for inducible clindamycin resistance. If a strain was found to be both erythromycin-resistant and clindamycin-resistant, it was considered to have constitutive clindamycin resistance. Overall, ten (3.9%) strains, including two methicillin-resistant S. aureus and eight methicillin-sensitive S. aureus, were found to be erythromycin-resistant and clindamycin-sensitive according to the disk diffusion method and all of these strains showed inducible clindamycin resistance by the D-test method. Constitutive clindamycin resistance was detected in 100 of all MRSA strains. In conclusion, the presence of inducible clindamycin-resistant strains may lead to clindamycin treatment failure in patients with S. aureus infection. The D-test method should be used for antimicrobial susceptibility testing of clindamycin.
  • [ X ]
    Öğe
    Carbapenem resistance in Acinetobacter baumannii: Where is it heading?
    (2013) Deveci O.; Dal T.; Tekin R.; Bozkurt F.; Tekin A.; Dayan S.
    In the context of the substantial increase in antibiotic resistance rates of Acinetobacter baumannii, we aimed to evaluate the susceptibility rate changes of A. baumannii strains for carbapenems. A. baumannii strains isolated from patients diagnosed with healthcare-associated infections between 2007-2010 were included. A total of 127 A. baumannii strains [53 (42%) from the intensive care unit and 74 (58%) from the non-intensive care unit] were isolated. Conventional methods and an automated microbiology system were used for identification. Susceptibility testing was studied by Kirby-Bauer disk diffusion method. In 2007, five of 26 strains, in 2008, 18 of 31, in 2009, 10 of 35, in 2010, and 20 of 35 were obtained from intensive care unit patients. The susceptibility rate for imipenem was 50% in 2007 but 20% in 2010, while for meropenem it was 55% in 2007 but 12% in 2010. Prevention and control of antibiotic resistance among Acinetobacter species needs antibiotic usage restrictions and infection control precautions.
  • [ X ]
    Öğe
    A case of acute hemorrhagic cystitis caused by salmonella paratyphi a in a pediatric patient
    (2012) Yula E.; Deveci O.; Toka-Ozer T.; Tekin A.; Inci M.; Karakus A.
    It is a very rare medical condition that Urinary tract infection (UTI) caused by Salmonella species. Seven-year-old boy admitted to our hospital with complaint of lower abdominal pain, burning and pain during urination (dysuria), nausea and increased fever. The patient had normal vital signs but abdominal examination revealed bilateral suprapubic tenderness. In the laboratory, it was found the amount of hemoglobin 12.9 g/dL, red blood cell count 4.8 million/mm3, white blood cell count 11.800/mm3, platelet count 275.000/mm3, level of C-reactive protein 30.2 mg/L, serologically S. paratyphi A "O" antibody (1/160) and S. paratyphi A "H" antibody (1/320) positivity. Urine examination showed gross hematuria and leukocyte esterase was positive. Urine culture was performed and isolate obtained urine culture was identified with conventional methods. Result of urine culture was reported as Salmonella species and isolate was determined as Salmonella paratyphi A by using anti-sera during the advanced identification. Results of radiological imaging were found normal. The patient was diagnosed as acute hemorrhagic cystitis caused by S. paratyphi A and received ceftriaxone treatment for seven days and had a full recovery. We conclude that in case of acute hemorrhagic cystitis, S. paratyphi A should be considered as causative agent in endemic areas.
  • [ X ]
    Öğe
    The distribution according to the species of Gram-negative bacteria isolated from hospitalized patients's urine specimens and their antimicrobial susceptibility
    (2012) Dal T.; Tekin A.; Tekin R.; Deveci O.; Can S.; Özekinci T.; Dayan S.
    Objective: In this retrospective study, we aimed to determine the distribution according to the species of Gram-negative bacteria in isolates obtained from urine cultures of hospitalized patients with urinary tract infections and to detect their antimicrobial susceptibility pattern for contribute to empirical treatment approach to urinary tract infections in our hospital. Methods: Between the dates of January 2006 and September 2011, a total of 3,548 Gram-negative isolates obtained from urine cultures of hospitalized patients with urinary tract infection were identified by conventional methods and the BD PhoenixTM 100 (Becton Dickinson, MD, USA) fully automated microbiology system. Antimicrobial susceptibility testing of isolates was performed by Kirby- Bauer's disk diffusion method according to the Clinical and Laboratory Standarts Institute (CLSI) criteria. In addition, extended-spectrum beta-lactamase (ESBL) production of predominant urinary pathogenic bacteria was detected by the double-disk synergy method. Results: In this study, a significantly higher incidence of urinary tract infection was observed in females compared with males; 2,245 (63.3%) and 1,303 (36.7%), respectively. Escherichia coli was the predominant pathogenic bacterium and accounted for 2,341 (65.8%) of 3,548 hospitalized patients with urinary tract infections. In addition, Klebsiella pneumoniae accounted for 679 (19.1%), Acinetobacter spp. for 177 (5%), Enterobacter spp. for 176 (5%), Pseudomonas aeruginosa for 142 (4%), and other Gram-negative bacteria for 38 (1.1%). Antimicrobial susceptibility rates of Gram-negative bacteria isolated from urine cultures for ceftazidime, trimethoprim-sulfamethoxazole, ciprofloxacin, piperacillin-tazobactam, cefoperazonesulbactam, amikacin, imipenem, meropenem were detected as 34.5%, 38%, 42%, 50%, 70%, 87%, 90%, and 92%, respectively. In addition, the rates of ESBLproducing Escherichia coli and Klebsiella pneumoniae were detected as 38% and 36%, respectively. Conclusion: The present study and the other studies show that in the near future it is possible our therapeutic options may become more limited than ever due to the development of high-level resistance against broad-spectrum antibiotics such as cephalosporins, carbapenems and fluoroquinolones. Due to the highlevel resistance to ceftazidime, ciprofloxacin and trimethoprim-sulfamethoxazole, we recommend that these antibiotics should not be used for the empirical treatment of urinary tract infections.
  • [ X ]
    Öğe
    [Fascioliasis and brucellosis in same patient].
    (2014) Deveci O.; Aslan E.; Tekin A.; Toka Özer T.; Tekin R.; Bozkurt F.; Çetinçakmak M.G.
    Brucellosis is a zoonotic infectious disease that can affect many organs and systems and leads to very different clinical circumstances. Brucellosis is rare in association with various infectious agents. Fascioliasis is a zoonotic disease caused by Fasciola hepatica, popularly referred to as a large leaf-shaped liver fluke. This case is a 39-year-old male patient, and his complaints began a week ago, which were chills, fever, abdominal pain, nausea, vomiting, weakness, sweating, and widespread pain. The patient was considered brucellosis in the preliminary diagnosis. Rose Bengal test and Wright test (1/640) were detected as positive. Due to patients having elevated liver enzymes, abdominal ultrasound was taken. A liver lesion was seen with abdominal ultrasound. So, abdominal computed tomography (CT) was taken. The CT result report came in the form that at the left lobe of the liver segment 2, largely necrosis that showed no contrast enhancement, approximately 61x63 mm in size (compatible with fascioliasis) is viewed. The patient's IHA test results, required for fascioliasis, were detected as 1/320 positive. Especially for zoonotic diseases in areas with high endemicity, it should be considered that more than one infectious agent can be present together in high-risk patients.
  • [ X ]
    Öğe
    In vitro antimicrobial susceptibility pattern among stenotrophomonas maltophilia strains isolated from clinical specimens
    (Duzce University Medical School, 2014) Tekin A.; Dal T.; Tekin R.; Deveci Ö.; Demirkaya S.; Mete M.; Dayan S.
    Aim: In this study, the determination of in vitro antimicrobial susceptibility rates among Stenotrophomonas maltophilia strains isolated from clinical specimens was aimed. Method: A total of 60 S. maltophilia strains isolated from various clinical specimens sent to Dicle University Hospital clinical microbiology laboratory between January 2006 and September 2011 were included retrospectively in this study. Urine samples were inoculated onto 5% sheep blood agar and Eosin-Methylene Blue (EMB) agar media, quantitatively; other clinical samples were inoculated, qualitatively. Identification of S. maltophilia isolates was performed by conventional methods and fully automated microbiology system. Antimicrobial susceptibility tests were performed by Kirby-Bauer’s disk diffusion method and by broth dilution. Results: A total of 60 S. maltophilia strains isolated from clinical specimens as 24 urine, 15 blood, 11 sputum, four synovial fluids, three cerebrospinal fluids, two wound swabs and one middle ear materials. The resistance rates against antibiotics were detected as 85% (n=51) for piperacillin-tazobactam, 75% (n=45) for levofloxacin, 73% (n=44) for ciprofloxacin, 57% (n=34) for trimethoprim-sulfamethoxazole, 5% (n=3) for meropenem and imipenem, and 0% (n=0) for amikacin. Conclusion: In this study, it was detected that piperacillin-tazobactam can be used as an alternative option other than trimethoprim-sulfamethoxazole, for the treatment of infections due to S. maltophilia at least in our region. © 2012 Düzce Medical Journal.
  • [ X ]
    Öğe
    In vitro efficiency of ertapenem among escherichia coli strains isolated from clinical specimens
    (2013) Tekin A.; Deveci Ö.; Dal T.; Tekin R.; Bacalan F.; Akpolat N.
    In this study, in vitro efficiency of ertapenem and extanded-spectrum beta-lactamase (ESBL) producing among Escherichia coli strains isolated from clinical specimens were investigated. 334 E. coli strains isolated from clinical specimens that sent to Dicle University Hospital clinical microbiology laboratory between December 2010 and August 2011 were included in this study. Identification of isolates was performed by conventional methods and a fully automated microbiology system. Antimicrobial susceptibility testing of strains was performed by Kirby- Bauer's disc diffusion method and E-test method. Double-disk synergy method was used for the detection of ESBL-producing. 334 E. coli strains were isolated from clinical specimens as 233 (69.8%) urine, 55 (16.4%) blood, 32 (9.6%) wound swabs and 14 (4.2%) others. In 177 (53%) from 334 E. coli strains were determined the ESBL activity. Ertapenem sensitivity rate among strains was determined as 93.4%. Although there was no resistance to ertapenem in ESBL-non-producing strains, ertapenem resistance rate was found as 12.4% in ESBL-producing strains. The high resistance rate for ertapenem in ESBL-producing strains according to the ESBL-non-producing strains was found to be statistically significant (p<0.001). In the light of present study, we think as the better option of ertapenem which has got a high sensitivity rate in the treatment with antimicrobial agents of infections caused by ESBL-producing E. coli strains at least in our hospital.
  • [ X ]
    Öğe
    Is chryseobacterium indologenes a shunt-lover bacterium? a case report and review of the literature
    (2013) Ozcan N.; Dal T.; Tekin A.; Kelekci S.; Can S.; Ezin O.; Kandemir I.
    Chryseobacterium indologenes is a non-fermentative Gram-negative bacillus formerly belonging to the Flavobacterium genus. It is widely found in water and soil, also on wet surfaces of the hospital environment. It rarely causes infections and is usually associated with altered immune status or indwelling devices. We present a case of ventriculoperitoneal shunt infection caused by C. indologenes in a premature pediatric patient. A sixmonth-old male infant with congenital hydrocephalus and ventriculoperitoneal shunt was admitted with complaints of irritability, high fever and projectile vomiting. He was diagnosed as suffering from meningitis based on the clinical symptoms and laboratory findings of cerebrospinal fluid. The ventriculoperitoneal shunt was externalized and cerebrospinal fluid samples were sent for bacterial cultures. The isolated bacterium was identified as C. indologenes by conventional methods and the BD Phoenix™ 100 (Becton Dickinson, MD, USA) fully automated microbiology system. Antimicrobial susceptibility testing was performed by the microdilution method and Kirby-Bauer's disk diffusion method according to the Clinical and Laboratory Standards Institute guidelines. The isolate was found susceptible to ciprofloxacin, levofloxacin, cefoperazone and trimethoprim-sulfamethoxazole, while it was resistant to amikacin, aztreonam, cefepime, ceftazidime, gentamicin, imipenem and ticarcillinclavulanic acid. The treatment was started with trimethoprim-sulfamethoxazole and cefoperazone-sulbactam The ventriculoperitoneal shunt was then removed. The patient was fully healed after two weeks and discharged. Central nervous system infection is a rare form of C. indologenes infections. The case presented herein may make a useful contribution to the existing literature.
  • [ X ]
    Öğe
    Molecular typing of nosocomial enterococci by pulsed-field gel electrophoresis
    (Refik Saydam National Public Health Agency (RSNPHA), 2015) Güldemir D.; Karagöz A.; Dal T.; Tekin A.; Özekinci T.; Durmaz R.
    Objective: Enterococci are the second most common cause of nosocomial urinary tract and wound infections, also third most common cause of nosocomial bacteremia. Currently, especially vancomycin-resistant enterococci (VRE) are one of the significant pathogens that cause nosocomial infections and increase mortality, morbidity, and healthcare costs. Therefore prevention and control of the nosocomial VRE outbreaks and epidemiological analysis of the infection are important. Pulsed-Field Gel Electrophoresis (PFGE) is accepted as a "gold standard" method in the molecular epidemiological analysis of enterococcal infections. The aims of this study are to determine the clonal relationship among the nosocomial enterococcal isolates and the rate of cross-contamination between them. Method: Thirty-six Enterococcus strains isolated from hospitalized patients with nosocomial infection in different clinics of Dicle University Hospital between November 2010 and June 2012 were included in this study. A total of 36 isolates were obtained from various clinical samples including urine (n=18), blood (n=6), bronchoalveolar lavage (BAL) fluid (n=5), wound biopsy sample (n=5), vaginal smear (n=1) and catheter tip (n=1). Nine of the thirty-six isolates were VRE. Isolation and identification of the isolates were conducted in the bacteriology laboratories of Dicle University Medical Faculty, Department of Medical Microbiology. The conventional methods and BD PhoenixTM 100 (Becton Dickinson, MD, USA) fully automated microbiology system were used for the identification. Antimicrobial susceptibilities of enterococcal strains were determined by a fully automated microbiology system according to the Clinical and Laboratory Standards Institute (CLSI). In addition, vancomycin susceptibilities of the isolates were performed by E-test method. Staphylococcus aureus ATCC 25923 strain was used for quality control. PFGE was studied for determining of rate of cross-contamination. PFGE was performed in National Molecular Microbiology Referance Laboratory, Public Health Institution of Turkey (PHIT). DNA restriction fragments were obtained by cutting bacterial DNA with the SmaI enzyme. DNA restriction fragments were used by CHEF DR II (Bio-Rad Laboratories, Nazareth, Belgium) system. PFGE results were evaluated by Bionumerics software system (version 6.01; Applied Maths, Sint-Martens-Latem, Belgium). Results: DNA patterns of the isolates were obtained by PFGE, and dendrogram of DNA patterns were made. Comparison of DNA patterns obtained by PFGE showed that 26 E. faecium strains were divided into four different clusters and one major group, 10 E. faecalis strains were three clusters and one major group. Twenty-six Enterococcus faecium isolates were involved in a joint cluster (97% similarity). The cluster rate was found to be 77% (20/26), number of E. faecium strains in each cluster ranged from 2 to 14 strains. Conclusion: In this study, cross-contamination was highlighted among enterococcal strains causing nosocomial infections in Dicle University Hospital between November 2010 and June 2012. Our data revealed that more effective infection control programs should be implemented to prevent cross-transmission.