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    Ace gene polymorphism and of MTHFR parameters effects on migraine
    (Elsevier Science Bv, 2015) Aluclu, M.; Unan, F.; Tekes, S.
    [Abstract Not Available]
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    Angiotensin-converting enzyme gene insertion/deletion polymorphism with metabolic syndrome in Turkish patients
    (Springer, 2013) Simsek, S.; Tekes, S.; Turkyilmaz, A.; Tuzcu, A. K.; Kilic, F.; Culcu, N. N.; Isik, B.
    Background: The ACE gene has received substantial attention in recent years as candidate for a variety of diseases. The most common polymorphism in ACE gene is the Insertion/Deletion (I/D, rs4646994) polymorphism located on intron 16. Aim: We investigated the association between metabolic syndrome (MS) and the insertion (I) -deletion (D) polymorphisms in the angiotensin converting enzyme (ACE) gene in south-east of Turkey. Subjects and methods: One hundred and sixty subjects, with 101 cases of MS and 59 age-and gender-matched healthy controls were included in the study. Results: The frequency of ACE I/D polymorphism was found to be 49.5% for DD, 36.6% for ID, and 13.9% for II in the MSstudy group and 44.1% for DD, 42.4% for ID and 13.5% for II in the control group. Allele frequencies were found to be 0.68% for D and 0.32% for I allele in the study group with MS and 0.65% for D, 0.35% for I allele in the control group. The I/D polymorphism of the ACE gene, DD, ID, and II genotypes occurred with similar frequencies in the study group with MS and the control group with no significant differences (p > 0.05). On applying one-way analysis of variance to different ACE gene polymorphic groups in patients with MS were not significantly associated to ACE gene polymorphism and waist circumference, systolic blood pressure, diastolic blood pressure, fasting blood glucose, HDL, and LDL (p > 0.05). Conclusions: Further studies of patients in larger numbers and of different ethnic backgrounds may be necessary to elucidate the association between the ACE I/D gene polymorphism and MS.
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    CHRONIC OBSTRUCTIVE PULMONARY DISEASE AND PARAOXONASE-1 192 AND 55 GENE POLYMORPHISMS
    (Taylor & Francis Ltd, 2010) Tekes, S.; Isik, B.; Yildiz, T.; Simsek, S.; Isik, M. R.; Budak, T.
    Chronic obstructive pulmonary disease (COPD) is a leading cause of chronic morbidity and mortality. The oxidative stress is increased in COPD patients. Paraoxonase (PON1) in the lung may have a role to protect from oxidative stress. We have investigated a possible relationship between PON1 55 and PON1 192 gene polymorphisms in COPD patients and control subjects. A Total of 62 inpatients of COPD, 45 non-smokers and 35 smokers without COPD were included in the study The serum levels of PON1 were measured. The PON1 genotypes were determined by PCR amplification of the region containing the polymorphism followed by restriction enzyme digestion. The serum levels of PON1 were significantly low in the COPD patients group (p<0.001). There were no statistical differences between the COPD and control groups for PON1 55 polymorphism. The PON1 192 QQ and QR genotypes occurred with similar frequencies in the COPD and control groups with no significant differences while a significant difference was found between the PON1 192 RR allele frequencies (p<0.05) of all groups. PON1 192 gene polymorphism may be considered associated with COPD. PON1 polymorphisms and low PON1 activity levels might be considered as an independent risk factor for COPD.
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    Cytogenetic and Y chromosome microdeletion screening studies in infertile males with Oligozoospermia and Azoospermia in Southeast Turkey
    (Springer/Plenum Publishers, 2008) Balkan, M.; Tekes, S.; Gedik, A.
    In view of the genetic risks for the next generation, the importance of careful evaluation of karyotypes and AZF microdeletions in male infertility prior to assisted reproduction is evident. In the present study, it is aimed to investigate the frequency and types of both major chromosomal abnormalities by using standard cytogenetic methods and Y chromosome microdeletions of infertile males with azoospermia and oligozoospermia to give appropriate genetic counseling before assisted reproduction techniques in southeast Turkey. A total of 80 infertile males (52 were azoospermic, 25 oligospermic and 3 asthenospermic) were studied for the cytogenetic evaluation and molecular AZF screening program prior to use of assisted reproduction techniques. A detailed history was taken for each man. Karyotyping was performed on peripheral blood lymphocytes according to standard methods. Polymerase chain reaction (PCR) amplification by using 15 Y-specific sequence-tagged sites of AZF region was performed to screen the microdeletions in the AZF region of Y chromosome. Of 80 cases, 71 had normal karyotype (46,XY). The total prevalence of chromosomal abnormalities was found to be 11.2% (9/80), including seven patients with Klinefelter syndromes and two patients with balanced autosomal rearrangements. All of the patients with Klinefelter Syndrome had azoospermia, but carriers with translocation had oligospermia. The deletions of Y chromosome were seen in one patient (1.3%) with features of normal karyotype and azoospermia. Microdeletions were seen in the AZFc and AZFd regions. Neither AZFa nor AZFb microdeletions were detected. The occurrence of chromosomal anomalies and Y chromosome microdeletions among infertile males strongly suggests the need for routine genetic testing and counseling prior to employment of assisted reproduction techniques.
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    The insertion/deletion polymorphism in the ACE gene and chronic obstructive pulmonary disease
    (Funpec-Editora, 2013) Simsek, S.; Tekes, S.; Oral, D.; Turkyilmaz, A.; Isik, B.; Isik, M. R.; Akkoc, H.
    An insertion/deletion (I/D) polymorphism was identified in intron 16 of the gene encoding the human angiotensin I-converting enzyme (ACE), a candidate gene for chronic obstructive pulmonary disease (COPD). We investigated the relationship between this polymorphism in the ACE gene and the risk of developing COPD. Sixty-six COPD in-patients and 40 non-smoking control individuals were recruited for this study. The distribution of ACE genotypes in these individuals was studied. The frequencies of ACE genotypes were found to be 47.0% for DD, 30.3% for ID, and 22.7% for II in the COPD group and 32.5% for DD, 47.5% for ID, and 20.0% for II in the control group. The allele frequencies were found to be 0.62% for the D allele and 0.38% for the I allele in the COPD group and 0.56% for the D allele and 0.44% for the I allele in the control group. A significant difference was found between I and D allele frequencies (P < 0.05) of the study and control groups. Our results suggest that this ACE polymorphism may be associated with the development of COPD.
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    P53 gene exon 4 RFLP polymorphism and relation with severity of disease in patients with coronary artery disease
    (Blackwell Publishing, 2006) Cakir, D. U.; Yokus, B.; Tekes, S.; Iltimur, K.; Mete, N.; Uysal, E.
    [Abstract Not Available]
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    Relation between angiotensin-converting enzyme I/D gene polymorphisms and modified shock index in patients with a first acute anterior myocardial infarction
    (Wiley-Blackwell, 2016) Ozturk, C.; Ozturk, O.; Ozturk, U.; Tekes, S.
    [Abstract Not Available]
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    The role of nitric oxide synthase (eNOS) gene polymorphism in coronary artery diseases and relation with severity of disease
    (Blackwell Publishing, 2006) Tekes, S.; Cakir, D. U.; Yokus, B.; Mete, N.; Iltimur, K.
    [Abstract Not Available]
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    Role of p53 codon 72 polymorphism in chromosomal aberrations and mitotic index in patients with chronic hepatitis B
    (Assoc Bras Divulg Cientifica, 2012) Akbas, H.; Yalcin, K.; Isi, H.; Tekes, S.; Atay, A. E.; Akkus, Z.; Budak, T.
    Polymorphisms of the p53 gene, which participates in DNA repair, can affect the functioning of the p53 protein. The Arg and Pro variants in p53 codon 72 were shown to have different regulation properties of p53-dependent DNA repair target genes that can affect various levels of cytogenetic aberrations in chronic hepatitis B patients. The present study aimed to examine the frequency of chromosomal aberrations and the mitotic index in patients with chronic hepatitis B and their possible association with p53 gene exon 4 codon 72 Arg72Pro (Ex4+ 119 G>C; rs1042522) polymorphism. Fifty-eight patients with chronic hepatitis B and 30 healthy individuals were genotyped in terms of the p53 gene codon 72 Arg72Pro polymorphism by PCR-RFLP. A 72-h cell culture was performed on the same individuals and evaluated in terms of chromosomal aberrations and mitotic index. A high frequency of chromosomal aberrations and low mitotic index were detected in the patient group compared to the control group. A higher frequency of chromosomal aberrations was detected in both the patient and the control groups with a homozygous proline genotype (13 patients, 3 control subjects) compared to patients and controls with other genotypes [Arg/Pro (38 patients, 20 control subjects) and Arg/Arg (7 patients, 7 control subjects)]. We observed an increased frequency of cytogenetic aberrations in patients with chronic hepatitis B. In addition, a higher frequency of cytogenetic aberrations was observed in p53 variants having the homozygous proline genotype compared to variants having other genotypes both in patients and healthy individuals.
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    THE TUMOR NECROSIS FACTOR-A (TNF-A) GENE-308 G/A POLYMORPHISM AND THE TUMOR NECROSIS FACTOR-RELATED APOPTOSIS-INDUCING LIGAND (TRAIL) GENE POLYMORPHISMS IN BEHCET'S DISEASE
    (Diagnosis Press Ltd, 2010) Isi, H.; Erdal, M. E.; Akdeniz, S.; Oral, D.; Ay, O. I.; Tekes, S.; Sula, B.
    Behcet's disease (BD) is a chronic, multisystemic inflammatory disease. The specific etiology of BD remains elusive, but the interaction between infectious-agent exposure and genetic factors may have a role. In this report, we aim to investigate the possible association between pathogenesis of the BD and TNF-alpha gene 308A/G (rs1800629) polymorphism and the TRAIL gene (Arg141His, G422A (rs6557634), Thr209Arg, C626G (rs20575) and Glu228Ala, A683C (rs20576)) polymorphisms in people from southeast of Turkey. The study population consisted of 55 BD patients and 80 healthy subjects. All samples were collected and studied between July 2009 and January 2010. Polymorphisms were detected by polymerase chain reaction restriction fragment length-polymorphism (PCR-RFLP) analysis. The patients and healthy control groups were similar with respect to their ages and sex characteristic. Statistically, there was not significant difference between the BD patients and healthy control groups in terms of TRAIL Arg141His, G422A (rs6557634) polymorphism, TRAIL Thr209Arg, C626G (rs20575), TRAIL Glu228Ala, A683C (rs20576) and TNF-alpha-308 G/A (rs1800629) polymorphisms. We could not detect statistically significant difference between the BD patients and healthy control groups according to TNF-a-308 G/A (rs1800629), TRAIL Arg141His, G422A (rs6557634), TRAIL Thr209Arg, C626G (rs20575) and TRAIL Glu228Ala, A683C (rs20576) gene polymorphism.