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Öğe Conjunctival impression cytology in patients with ectodermal dysplasia(Galenos Yayincilik, 2012) Soker, Sevda; Keklikci, Ugur; Mese, Ayse; Akkus, Murat; Nergiz, YusufEctodermal dysplasia is a group of symptoms affecting tissues and organs of ectodermal origin. In this study, conjunctival impression cytology technique which is a non-invasive method was performed in patients with ectodermal dysplasia to evaluate the affects on conjunctiva. Six eyes of three patients with ectodermal dysplasia who were followed up by the Ophthalmology Clinic of Dicle University Faculty of Medicine and Prosthetic Dentistry Departments were included in the study. Bulbar conjunctiva impression cytology method was applied to the upper surface of the bulbar conjunctiva and the cell groups taken to the surface of cellulose acetate paper were fixed by a solution containing 70% ethyl alcohol, 37% formaldehyde and glicial acetic acid with a ratio of 20:1:1 and later stained by periodic acid-schiff and haematoxylin-eosin. Using impression cytology method a decrease in integrity of the cells, picnotic changes in the nucleus, epithelial cell-like changes and loss of goblet cells were observed in patients with ectodermal dysplasia. The findings of eye dryness in patients with ectodermal dysplasia were supported by the impression cytology technique. Impression cytology can be used as a non-invasive method in ophthalmologic follow-up of patients with ectodermal dysplasia. (Turk Arch Ped 2012; 47: 69-72)Öğe The Demonstration of Changes in Bulbar Conjunctiva Surface Epithelium in the Psoriatic Patients Treated With PUVA(Amer Soc Contemporary Medicine Surgery & Ophthalmology, 2008) Soker, Sevda; Nergiz, Yusuf; Cakmak, Sevin; Bahceci, Selen; Aytekin, SemaWe investigated the effects of disease itself and PUVA treatment on surface epithelium of conjunctiva in psoriatic patients (PP) before PUVA and after PUVA therapy and in 32 healthy volunteers. Squamous metaplasia was detected in PP both before and after PUVA therapy. We concluded that PUVA treatment applied together with preventive measures, would lead to less severe ocular side effects.Öğe The Effects of Electromagnetic Fields on the Ultrastructure of Heart(Soc Chilena Anatomia, 2011) Soker, Sevda; Sert, Cemil; Deniz, Mustafa; il Tekmen, Is; Akkus, Murat; Nergiz, YusufIncreasingly the use and convenience of electrical appliances in our daily lives are the cause of harmful effects caused by electromagnetic fields (EMF). The aim of this study was to research the effect of EMF on the ultrastructure of the heart in EMF exposed rats. In this study 45 male Sprague Dawley rats ranging in weight between 260 and 280 grams were used. The rats were divided into 3 groups, control (n:15), Sham (n:15) and EMF group (n: 15) and exposed for 14 days 3 hours per day; gauss levels at 2.5 were applied to the EMF group, while the sham group in the same environment in Plexiglas cage was kept for 14 days 3 hours per day without magnetic field exposure. Control group at 14/10 hours light dark cycle fed in normal cages for 14 days. After two weeks rats were sacrificed by 50mg/kg of Ketalar anesthesia and heart tissue fixed in 2.5 gluteraldehide. Routine follow up with electron microscopic assessment. Mitochondrial structures and cellular structures observed in all the groups were normal. Myofibrillar loss, dilatation of smooth endoplasmic reticulum, mitochondrial swelling or cristalysis was not observed. Intercalated disc degeneration and apoptosis of nucleus was not observed. Therefore, and as a result of our study we did not observe differences between control and EMF groups.Öğe Effects of Nicotine on Rat Tongue Muc Histopathological and Immunohistochemical Analysis(Sci Printers & Publ Inc, 2016) Eratilla, Veysel; Uysal, Ibrahim; Deveci, Engin; Gunes, Rojdan Ferman; Eratilla, Elif; Yildiz, Ismail; Soker, SevdaOBJECTIVE: To evaluate the histopathological and immunohistochemical effects of systemically administered nicotine on rat tongue mucosa. STUDY DESIGN: Rats were assigned to groups: the experimental group received nicotine ically (nicotine sulphate 2 mg/kg subcutaneously for 28 days), while the rats in the trol grow ere administered physiological saline mL subcutaneously for 28 days). All animals sacrificed at the end of the study, and tongue tissue ere removed and prepared according his histological procedures. Sections were sta toxylin a sin and observed by. Immunorec y of tongue mucosa , 'E-ca Ilagen IV, and VEr by immunhistochemical staining. RESULTS: There w if cant differences in the average histlogical a between the nicotine-treated treated gr Morphological changes, including inflammatory I to infiltration and cellular desquamation, blood dilation, hemorrhage, and epithelial degeneration r. re noted. Further, E-cadherin expression w significantly decreased in the nicotine-treated grow,w the untreated group. The nicotine treat up showed an increase in collagen IV secondary ae and basal cells. CONCLUSION: The increased level of VEGF exprescs n in the nicotine-treated group may have affected endothelial cell apoptosis.Öğe Effects of Polymeric Zinc Propylen-Bis-Dithiocarbamate (Propineb) on Nasal Mucosa in Rats(Soc Chilena Anatomia, 2016) Samanci, Baver; Arslan, Eda; Samanci, Seyla Bolukbasi; Caypinar, Basak; Deveci, Engin; Soker, Sevda; Seker, UgurThe objective of this study was to evaluate the histopathologic and immunohistochemical effects of propineb on rat nasal mucosa. Twenty adult Sprague-Dawley rats weighing 180-220 g, were used as experimental animals. The rats were divided into propineb and control groups. The control group received distilled water with spray at the same time period. The experiment was terminated after three weeks. In each case, sections of the nosewere taken. In experimental group, microscopic examination of nasal respiratory mucosa revealed that degenerative changes in epithelium were observed in sections of propineb-treated group. There were also leukocyte infiltration and vascular dilatation detected in the connective tissue. We detected CD34-immunoreactive mononuclear cells and endothel cells in the lamina propria of propineb group. In propineb group compared to the control group, the respiratory epithelium, goblet and basal cell nuclei were stained positive for PCNA. Propineb inhalation may be irritating to the nasal mucosa.Öğe The Effects of Pomegranate and Carvacrol on Methotrexate-Induced Bone Marrow Toxicity in Rats(Canadian Soc Clinical Investigation, 2014) Sen, Velat; Bozkuri, Mehtap; Soker, Sevda; Ece, Aydin; Gunes, Ali; Uluca, Unal; Soker, MuratPurpose: The aim of this study was to evaluate the e ff ects of pomegranate (PMG) extract and carvacrol (CARV) on methotrexate (MTX)- induced oxidative stress and bone marrow toxicity. Methods: Wistar albino rats (32 rats) were divided into four groups (n=8): Group 1 was control; Group 2 was given a single intraperitoneal injection of methotrexate (20 mg/kg); Group 3 was treated with carvacrol (73 mg/kg i.p.) one day before MTX (20 mg/kg i.p.) injection; and, Group 4 received a single dose of MTX (20 mg/kg i.p) while PMG was administered orally for seven days at 225 mg/kg. After animals were euthanized, blood samples were taken to evaluate hematological parameters and oxidative stress. In addition, the femur was cropped and bone marrow was extracted for examination. Results: White blood cell count, hemoglobin, hematocrit and platelet count were found to be decreased in the MTX group, but these changes were prevented in the groups that received CARV and PMG. Furthermore, decreased bone marrow cellularity was found in the groups treated with MTX, whereas the PMG and CARV groups had cellularity similar to controls. Strikingly, oxidative stress increased in the MTX group, but was ultimately decreased in the rats that received the antioxidants PMG and CARV. Conclusion: Carvacrol and PMG were found to be protective against methotrexateinduced oxidative bone marrow damage. Use of these antioxidants, in combination with chemotherapeutics, may help to reduce some adverse effects of methotrexate.Öğe Effects of Sildenafil on Dental Pulp: Immunohistochemical and Ultrastructural Evaluation(Soc Chilena Anatomia, 2016) Soker, Sevda; Deveci, Engin; Yaman, Ferhan; Atilgan, Serhat; Ipek, Fikret; Uysal, IbrahimSildenafil is a strong peripheral vasodilator and is used to treat cardiovascular and neurosurgery. The purpose of this study was to investigate the immunohistochemical and ultrastructural effects of sildenafil on dental pulp of rats. The study was performed with adult female Wistar-Albino rats. Control group (n=7) were fed on standard laboratory diet until surgery. The study group (n=7) were administered sildenafil orally with orogastric tube 10 mg.kg(-1) once a day for 30 days. Each rat was anesthetized and incisor teeth were removed. This study examined the immunohistochemical and ultrastructural effects of sildenafil on the dental pulp in rats. The relaxation from the vessel, endothelial cell hyperplasia, moderate degeneration of collagen fibers were observed to cause degenerative changes in odontoblast with sildenafil. In the pulp tissue long-term use sildenafil is thought to cause degeneration and new vessel formation.Öğe Effects of Sildenafil on Dental Tissue(Medwell Online, 2011) Yaman, Ferhan; Soker, Sevda; Atilgan, Serhat; Erol, Behcet; Alp, Harun; Agacayak, Serkan Kamil; Gunay, AhmetTo investigate the effects of Sildenafil on dental tissue. The study was performed with adult female Wistar-Albino rats. Control group (n = 7) were fed on standard laboratory diet until surgery. The study group (n = 7) were administered Sildenafil orally with orogastric tube 10 mg kg(-1) once a day for 30 days. Each rat was anesthetized and mandibular bone with incisor teeth and soft tissue were removed. Dental pulp, dentin, periodental ligament, periodental soft tissue and bone were examined histologically. Neovascularization on the dental pulp and gingiva were significantly higher in the study group. Sildenafil can be used as a supporting factor in dental tissue healing.Öğe Electron Microscopic and Histological Evaluation of the Levator Muscle in Patients with Congenital Blepharoptosis(Sci Printers & Publ Inc, 2016) Soker, Sevda; Cakmak, Sevin; Nergiz, Yusuf; Deveci, Engin; Gul, Mehmet; Soker, Murat; Akkus, MuratOBJECTIVE: To examine the levator muscle in patients with congenital blepharoptosis and to investigate the relationship between those findings and age, gender, and degree of blepharoptosis. STUDY DESIGN: The levator muscles of 13 congenital ptosis patients who had undergone levator muscle resection were examined by light and electron microscopy. During the preoperative period the ptosis amount, levator function, tear function, Bell's phenomenon, and jaw-wink phenomenon were evaluated. RESULTS: There were 9 (69.2%) male and 4 (30.8%) female cases, with an average age of 10.61 +/- 4.77 years (range, 4-19). The quality and quantity of the levator muscle fibrils were assessed by histological examination. There was no relationship detected between histological features of levator palpebralis superior muscle and patients' age and gender (p < 0.05). Patients with a weak levator palpebralis superior muscle had fatty degeneration histologically. Higher levator palpebralis superior muscle function correlated with decreased fatty degeneration and increased skeletal muscle fibrils, but these findings were not statistically significant. There was a reduction in the diameter of muscle fibers and nucleus deformation when muscle function was examined in patients under low-magnification electron microscope. There were lipid droplets of various sizes in some sections, and the mitochondria were seen in mitochondrial crystalline structure depending on cytopathies. In some sections of this group there was large mitochondria and edema formation due to an increase in the mitochondrial matrix. CONCLUSION: More ultrastructural studies in larger populations are needed to support the relationship between structure and function of levator palpebralis superior muscle in patients with congenital blepharoptosis.Öğe Intracellular Ca2+ levels in rat ventricle cells exposed to extremely low frequency magnetic field(Taylor & Francis Inc, 2011) Sert, Cemil; Soker, Sevda; Deniz, Mustafa; Nergiz, YusufObjective: Electromagnetic fields can affect intracellular Ca2+ levels. The aim of this study was to determine the changes intracellular Ca2+ concentration in cardiac ventricle cells of rats exposed to 0.25 mT (2.5 Gauss) magnetic field. Methods: Forty-five male rats were introduced to this study. The rats were divided into three groups: control, sham, and experiment. The experimental group was exposed to 0.25 mT extremely low frequency (ELF) magnetic field for 14 days, 3 h/day. The sham group was treated like the experimental group, except for elf-magnetic field exposure. The control group was not subjected to anything and differed from the experimental group and sham group. In the end of experiment, rats were sacrificed, cardiac tissue was removed, and these were fixed in 10% neutral formalin. Then, ventricular cells were stained by Alizarin red staining method. Results: In the light microscopic examinations of control groups, in myofibril structures between groups, changes were not observed. In myofibril regions of the experimental group compared to other groups, increased heterogen Ca2+ accumulations were found. Conclusion: ELF magnetic fields are used in daily life. The results of this study show that intracellular Ca2+ accumulation in cardiac ventricles can increase in rats exposed to ELF magnetic field.Öğe INVESTIGATION OF SFLT-1 AND VEGF EXPRESSION IN NORMOTENSIVE AND PREECLAMPTIC PLACENTA. AN IMMUNOHISTOCHEMICAL STUDY(Rojan GÜMÜŞ, 2020) Sahin, Firat; Akkuş, Murat; Şeker, Uğur; Soker, Sevda; Gokalp-ozkorkmaz, Ebru; Ağaçayak, Elif; Aşır, FıratObjectives: The pathogenesis of preeclampsia is still not clear, but endothelial dysfunction believed to be one of the most encountered problems during placenta development in preeclamptic patients. Both vascular endothelial growth factor (VEGF) and its antagonist, soluble Fms-Like tyrosine kinase-1 (sFlt-1), have roles in vascular function. In this study, we have investigated immunohistochemical expression of VEGF and sFlt-1 in term placenta of normotensive and preeclampsia patients. Methods: Totally twenty term placentas were obtained from pregnant women of which 10 preeclampsia patient and 10 normotensive. Placentas were dissected and tissue samples were subjected to routine tissue processing protocol, then embedded in paraffin blocks. Serial sections were obtained from paraffin blocks and stained with H&E and PAS for routine histopathology. VEGF and sFlt-1 immunohistochemistry was performed to the sections. Results: When compared to control group, severe pathological changes were observed in preeclamptic placentas. Increase in number of syncytial knots and intervillous bridges, hemorrhage in interstitium, dilatation and congestion in villous capillaries, increase in fibrin accumulation in villus stroma, and increase in thickening of basement membrane were very clear. VEGF expression was significantly higher in normotensive placentas compared to preeclamptic. On the other hand, sFlt-1 expression was significantly increased in preeclamptic placentas. Conclusions: When the VEGF and sFlt-1 expression was considered, higher expression of sFlt-1 at preeclampsia, but decrease in VEGF expression, might be related to endothelial dysfunction in preeclampsia. Overall, this study demonstrates, imbalance between VEGF and sFlt-1 is one of the major reason of endothelial dysfunction in preeclamptic placenta.Öğe Investigation of the toxic effects of ropivacaine corneal endothelium by the impression cytological method(Saudi Med J, 2007) Soker, Sevda; Cakmak, Sevin; Olmez, Gonul; Buyukbayram, Huseyin; Nergiz, YusufObjective: To investigate the toxic effects of ropivacaine on corneal endothelium by using the impression cytological method. Methods: The study was performed between October and December 2004 in Dicle University Hospital, Diyarbakir, Turkey. Twenty-four eyes from 12 rats were used for the research. They were divided into 4 groups, each containing 3 different ropivacaine concentrations and a control. Immediately after enucleation, the corneas were excised and the endothelium was exposed to unpreserved ropivacaine 0.01, 0.1, or 1% and balanced salt solutions (BSS) as a control (6 corneas/group) for 20 minutes. The specimens were obtained by impression cytology method and stained with periodic acid shift. Then, they were examined under light microscope. Results: Blurring at cell membrane borders, vacuolization at cell cytoplasm, hydropic degeneration and increase in toxic granulation were observed in the 1% ropivacaine group. Cytoplasmic hydropic degeneration was determined in the 0.1% ropivacaine group. Cell structures were normal and almost identical to the control group in the 0.01% ropivacaine group. Conclusion: In this study, 2 major conclusions were determined. The impression cytology method can be used in examination of corneal endothelium, and exposure of rat corneal endothelium to 0.01% ropivacaine solutions in vitro appears to be nontoxic.Öğe Preventive Effects of Dexmedetomidine on the Liver in a Rat Model of Acid-Induced Acute Lung Injury(Hindawi Ltd, 2014) Sen, Velat; Guzel, Abdulmenap; Sen, Hadice Selimoglu; Ece, Aydin; Uluca, Unal; Soker, Sevda; Dogan, ErdalThe aim of this study was to examine whether dexmedetomidine improves acute liver injury in a rat model. Twenty-eight male Wistar albino rats weighing 300-350 g were allocated randomly to four groups. In group 1, normal saline (NS) was injected into the lungs and rats were allowed to breathe spontaneously. In group 2, rats received standard ventilation (SV) in addition to NS. In group 3, hydrochloric acid was injected into the lungs and rats received SV. In group 4, rats received SV and 100 mu g/kg intraperitoneal dexmedetomidine before intratracheal HCl instillation. Blood samples and liver tissue specimens were examined by biochemical, histopathological, and immunohistochemical methods. Acute lung injury (ALI) was found to be associated with increased malondialdehyde (MDA), total oxidant activity (TOA), oxidative stress index (OSI), and decreased total antioxidant capacity (TAC). Significantly decreased MDA, TOA, and OSI levels and significantly increased TAC levels were found with dexmedetomidine injection in group 4 (P < 0.05). The highest histologic injury scores were detected in group 3. Enhanced hepatic vascular endothelial growth factor (VEGF) expression and reduced CD68 expression were found in dexmedetomidine group compared with the group 3. In conclusion, the presented data provide the first evidence that dexmedetomidine has a protective effect on experimental liver injury induced by ALI.Öğe The Prophylactic Effects of Folic Acid and Vitamin E against Valproic Acid During Fetal Thymus Development: an Ultrastructural Study(Soc Chilena Anatomia, 2011) Baran, Ozlem Pamukcu; Tunik, Selcuk; Akkoc, Hasan; Devieci, Engin; Ayaz, Ercan; Soker, Sevda; Tas, Sevgi KalkanliTo evaluate histopathologic differences in the thymus of Wistar Albino rat fetuses prenatally exposed to valproic acid (VPA), folic acid (FA) and vitamin E (Vit-E). VPA (400 mg/kg), FA (400 mcg/kg) and Vit -E (250 mg/kg) were administered to rats on each of gestation days 8,9 and 10. The fetuses (n:24) were divided into four groups: control, VPA, VPA+Vit-E and VPA+FA groups. On the 20th day of gestation, all pregnant rats were sacrificed and the fetuses were extracted. Thin sections from thymus of live fetuses were stained with uranyl acetate-lead citrate and were examined under transmission electron microscope. The histopathological findings of control group was normal. In VPA group, it showed extensive degenerative changes by VPA were on all tissue compartments when compared to controls. In VPA-FA group, vacuoles, mitochondrial cristalysis and swelling were decreased in cytoplasm. In VPA-Vit-E group, lipid storage and vacuolization were observed. Mitochondrial cristalysis decreased. Our aim in the present study is to analyze histopathological changes which may occur in a high risk experimental model after giving of VPA. In addition, protective roles of the administration of FA and Vit-E are assessed.Öğe Reduced bone mineral density in children and adolescents with hemophilia from east Turkey(Wiley-Blackwell, 2016) Soker, Murat; Aktar, Fesih; En, Velat; Uzel, Hulya; Akpolat, Veysi; Soker, Sevda[Abstract Not Available]Öğe Trolox is more successful than allopurinol to reduce degenerative effects of testicular ischemia/reperfusion injury in rats(Elsevier Sci Ltd, 2020) Seker, Ugur; Nergiz, Yusuf; Aktas, Ayfer; Akkus, Murat; Ozmen, Mehmet Ferit; Uyar, Emre; Soker, SevdaIntroduction Reperfusion surgery following testicular ischemia is a reproductive health threatening status and may result with organ dysfunction in men. The high level of reactive oxygen species (ROS) and cease of blood flow to the testis are the most important reasons of this testicular injury. Until today, numerous experimental studies reported that antioxidants might be efficient to alleviate oxidative stress induced organ dysfunction. For this purpose, in this study, we have investigated the protective effects of xanthine oxidase (XO) inhibitor, allopurinol, and ROS scavenger, trolox, in a comparative perspective in testicular ischemia reperfusion injury subjected rats. Materials and methods Twenty-eight adult male Sprague Dawley rats were divided into four groups of seven animals in each; control, ischemia/reperfusion (I/R), allopurinol and trolox. The rats in control group did not receive any application. Animals in I/R, allopurinol and trolox groups were subjected to 2 h testicular reperfusion injury following 5 h ischemia. Intraperitoneally (i.p.) 1 ml isotonic, 200 mg/kg allopurinol and 50 mg/kg trolox were administered to the animals in these groups 30 min prior reperfusion. At the end of experiment, all animals were sacrificed and blood serum malondialdehyde (MDA) levels were measured. Histological sections were obtained from the testis and stained with hematoxylin and eosin (H&E), proliferating cell nuclear antigen (PCNA) and cleaved caspase-3. Apoptotic index was evaluated with TUNEL Assay. Results Severe morphological degenerations, increased serum MDA, cleaved caspase-3 and TUNEL Assay positivity rate, but reduced PCNA positivity rate was observed in ischemia and reperfusion group. Morphological degenerations, MDA level, apoptotic index and PCNA positive cell rate were slightly alleviated in allopurinol administered animals compared with ischemia and reperfusion group. Protection with trolox was more successful and the results of the analysis were similar to the control group. Discussion Ischemia that leading to testicular torsion is a reproductive health affecting problem and current surgical treatment methods might be insufficient to recover testis. Various types of ROS generating mechanisms in cell are limiting protective potency of allopurinol, and cocktail administration of different ROS inhibitors might be more effective. However, our results indicate that free radical scavenger trolox might be a candidate drug to alleviate degenerative effects of testicular ischemia reperfusion injury. Conclusions This is the first study that demonstrates antioxidant trolox was more successful than XO inhibitor allopurinol to protect testis against ischemia and reperfusion injury in rats. [GRAPHICS]
