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    Bulblets regeneration from in vitro improved leaf of Fritillaria imperialis L. and F. persica L.
    (Elsevier Sci Ltd, 2013) Kizil, Suleyman; Khawar, Khalid Mahmood; Sesiz, Ugur
    [Abstract Not Available]
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    Öğe
    IMPROVED in vitro PROPAGATION OF Hyacinthus orientalis L. USING FRUITS CONTAINING IMMATURE ZYGOTIC EMBRYOS AND TENDER LEAF SHEATH AS EXPLANTS
    (Univ Life Sciences Lublin, 2016) Kizil, Suleyman; Sesiz, Ugur; Khawar, Khalid Mahmood
    Hyacinthus genus is an important group of ornamental plants that bear white, yellow, pink, red or purple coloured flowers. It has about 2000 species spread around the world that are grown commercially. Although, plant occurs naturally in Turkey yet efforts have not been made to adapt it for open field cultivation. There is need to transfer and establish these plants from wild to fields for commercial use through in vitro and ex vitro approaches, that will help local economy profitably. This study reports in vitro culture of Hyacinthus orientalis L. subsp. orientalis; using fruits containing immature zygotic embryos cultured on MS medium containing varying concentrations of Thidiazuron (TDZ) with and without 0.2 mg l(-1) naphthaleneacetic acid (NAA) supplemented with 20 or 40 g l(-1) sucrose. The study also reports induction of bulblets on tender leaf sheaths on MS medium containing different concentrations of benzylaminopurine (BAP) + 0.1 mg l(-1) NAA supplemented with 30 g l(-1) sucrose. The maximum bulblet regeneration (40%) with 31.33 bulblets/explant was noted on MS medium containing 0.15 mg l(-1) TDZ supplemented with 40 g l(-1) sucrose. Whereas, the best bulblet regeneration on tender leaf sheath explants was noted on 1.5 mg l(-1) BAP + 0.1 mg l(-1) NAA with 2.97 bulblets per explant of 0.55 cm bulb diameter and 1.20 leaves per bulblet. These bulblets were cultured singly on MS medium containing 20 mg l(-1) GA(3) (Gibberellic acid) + 50 g l(-1) sucrose and attained a diameter of 0.75-1.00 cm in 30 days time. The bulbs regenerated on both explants were successfully rooted and acclimatised in plant growth chamber using peat moss followed by their transfer to open field conditions.
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    Öğe
    In Vitro Micropropagation of Fritllaria imperialis and F. persica using Different Portions of Leaf as Explant
    (Univ Agriculture, Fac Veterinary Science, 2024) Kizil, Suleyman; Khavar, Khalid Mahmood; Sesiz, Ugur
    Fritillaria imperialis and F. persica are important ornamental and medicinal plant species that grow naturally in large areas of the Middle East, Central, and Western Asia. These are counted as neglected crops and face lack of appropriate agronomic and biotechnological attention in development of propagation technologies. Therefore, the study aimed to micro propagate these two species using new explants of apical, middle, and basal portions of leaf from newly germinated seedlings for direct bulblet regeneration. The results testified the regeneration competency of the three explants of each species on six sets of naphthalene acetic acid (NAA) - benzyl aminopurine (BAP) modified Murashige and Skoog (MS) medium. The maximum number of 2.48 and 2.04 bulblets per explant on F. imperialis and F. persica was induced on basal portions of leaf on MS medium modified with 0.15 mg L-1 NAA+2.0 mg L-1 BAP and 0.1 mg L-1 NAA+2.0 mg L-1 BAP respectively. The regenerated bulblets were rooted on MS medium modified with 0.5 mg L(-1 )NAA and acclimatized in pots containing peat moss. Regardless of the explant types obtained from each species, all explants had the competence to regenerate new bulblets. A comparative analysis of morphological features of in vitro-grown plants with field-grown plants showed similarities. It was concluded that these methodologies could be exploited further for commercial micropropagation of both species without compromising the quality of the mother species.