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Öğe Effects of ganoderma lucidum and melatonin on sperm in rats exposed to electromagnetic field(Ijrr-Iranian Journal Radiation Res, 2021) Ozmen, M. F.; Seker, U.; Yavas, M. C.; Cirit, U.; Akdag, M. Z.Background: The aim of this study was to investigate the effect of the electromagnetic field (EMF) generated by 10 kV (50 Hz) high voltage line on the epididymal sperm characteristics and the protective effects of ganoderma lucidum (GL) and melatonin (M) in EMF exposed male rats. Materials and Methods: In this study, 64 Wistar rats were divided into 8 groups (n= 8). Rats in 26-day and 52-day experiment groups were exposed to EMF for 8 hours daily during 26 and 52 days respectively. Rats in EMF+GL-26 and EMF+GL-52 groups were treated with GL by oral gavage 20 mg/kg daily and rats in EMF+M-26 and EMF+M-52 groups received M 10 mg/kg daily during experiments. At the end of experiments, all rats were sacrificed and epididymal sperm concentration, motility and morphology were evaluated. Results: In the 26-day experiment, sperm motility of the control group were significantly lower than all groups (P<0.05). In the 52-day experiment, the EMF+GL52 group had the lowest tail and total morphologic defect rates and the differences between this group and EMF52 and EMF+M52 groups were significant. Conclusion: Our data demonstrated that; (1) EMF exposure can have different effects on sperm quality according to duration of exposure, (2) treatment of GL in rats exposed to EMF for 26 or 52 days increased sperm concentration and reversed negative effect of EMF on sperm morphology, respectively and (3) treatment of M in rats exposed to EMF for 26 or 52 days had negative effects on sperm concentration and sperm morphology, respectively.Öğe THE IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL EVALUATION OF PERICYTES IN HUMAN FULL TERM PLACENTAS OF GESTASYONAL DIABETES MELLITUS(Carbone Editore, 2013) Deveci, E.; Soker, S.; Turgut, A.; Aktas, A.; Ayaz, E.; Sak, S.; Seker, U.Pericytes, vessel wall plays a stabilizing role in the regulation of blood flow in the microcirculation. The purpose of this study is based on non-diabetic pregnancies complicated by gestational diabetes pregnancies to investigate the morphological structure of pericyte cells. In this study, as a control group human placental tissues from normotensive pregnancies was collected from diabetic wome at 28-35 weeks of gestation. Pericytes with smooth alpha-actin positive cells, endothelial cells, and painted like a belt was surrounded. Pericytes, capillary plexus and endothelial cells of large vessels in the mesenchyme around the middle shows desmin positive reaction. Placental micro vessels examined by transmission electron microscopy showed many pericytes. Placentas of gestational diabetes group, heterochromatin nucleus hypertrophy, dilatation of endoplasmic reticulum, mitochondria cristae in length, shortening was observed thickening of the filamentary structure. The contractile function of the barrier formed by endothelial cells, pericytes and can increase the contractions were consideredÖğe Investigation of the protective effects of melatonin, amifostine (WR-2721), and N-acetylcysteine on radiotherapy-induced uterine tissue injury in rats(Ijrr-Iranian Journal Radiation Res, 2020) Seker, U.; Aktas, A.; Nergiz, Y.; Zincircioglu, S. B.; Ketani, M. A.Background: The aim of this study was to investigate the protective potency of melatonin, amifostine (WR-2721), and N-acetylcysteine (NAC) when administered intraperitoneally (i.p.) 15 min before 10-Gy single-fraction radiotherapy. Materials and Methods: In this study, 35 female Sprague Dawley rats were divided into five groups of seven rats each. The rats in the control group did not receive any treatments. Rats in the radiotherapy, melatonin, amifostine, and NAC groups underwent abdomino-pelvic irradiation with 10-Gy single fraction gamma (gamma) irradiation. Melatonin 50 mg/kg, amifostine 200 mg/kg, and NAC 500 mg/kg were i.p. administered to the rats 15 min before irradiation. Animals were sacrificed 48 h after irradiation. Uterus samples were collected and, routine histopathological tissue processing was performed. Sections from tissue samples were stained with H&E and analyzed with the terminal deoxynucleotidyl transferase dUTP nick end labelling method (TUNEL assay). Results: Severe morphological degenerations and increases in the apoptotic index (AI) were observed in the radiotherapy group. Tissue protection and AI reduction were observed in the amifostine and NAC groups. Melatonin was more effective than amifostine and NAC. Morphological damage was almost completely repaired, and the AI of the melatonin group was quite similar to that of the control group. Conclusion: This experiment failed to determine a more successful administration technique of amifostine. The protective effects of amifostine and NAC were similar. Melatonin was more successful than these two drugs, and might be an alternative to amifostine when time, dose, or adverse effect constraints are encountered.Öğe Morphological Changes Caused by Streptozotocin-Induced Diabetes in the Healthy Gingiva of Rats(Johann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh, 2016) Dag, A.; Firat, E. T.; Uysal, E.; Ketani, M. A.; Seker, U.Background and Objective: Epidemiologic and clinical studies have indicated that diabetes is a risk factor for periodontal disease progression. The aim of the present study was to evaluate the morphological changes of gingiva in streptozotocin diabetic rats. Material and Methods: 30 male Wistar rats that weighed 250-300g were used in this study. The animals were randomly divided into 2 groups, one with streptozotocin-induced diabetes and another one with healthy (non-diabetic) animals. All rats were sacrificed after 21 days, and their maxillary first molars with surrounding tissues were observed morphological analyses. Results: In this study, it was observed that the epithelial thickness was greater in the diabetes group, compared to the control group. The statistical comparison of the diabetes and control groups for the thickness of each of the layers of the epithelium demonstrated that the thickness of the keratinized (corneum), granular and basal layers had significantly increased in the diabetic animals. Furthermore, the diabetes group displayed a decrease in the height of the connective tissue papillae, which was found to be statistically insignificant. Another important finding detected in the diabetes group was the congestion of the gingival capillaries, which showed that blood circulation is impaired in diabetes cases. Conclusion: On the basis of the results obtained in this study, it was concluded that streptozotocin-induced diabetes may increase predisposition to periodontal disease by causing morphological changes in the periodontal tissues.
