Yazar "Seckin, Kerem Doga" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    Effect of nicotine exposure during gestation on neonatal rat ovaries
    (E-Century Publishing Corp, 2016) Gorkem, Umit; Togrul, Cihan; Seckin, Kerem Doga; Karsli, Mehmet Fatih; Deveci, Engin; Gungor, Tayfun
    The purpose of present study was to investigate effects of nicotine on follicular development in the neonatal rats. In this study, 14 adult Sprague-Dawley rats weighing 230-260 g (+/- 10 g) were used as experimental animal. After detecting pregnancies with vaginal smear method, pregnant rats were two equal groups were separated. The pregnant females were treated with the nicotine for 21 days and litters were sacrificed at first day of birth. The ovaries of litters were processed through routine paraffin embedding method, serially sectioned by microtome, stained with hematoxylin-Eosin and immunohistochemical technique investigated by light microscope. In the ovary of group treated nicotine, Preventing follicular development was observed in granular degeneration and luteal cells. It was also observed in inflammation and bleeding vessels. Immunohistochemical localization of vimentin was restricted to the granulosa cells and stromal area. Nicotine treatment group in granular cells and luteal cells in E-cadherin expression was weak. We concluded that nicotine might affect the cellular junctions in the ovarian follicular development. The high intensity in the staining with vimentin observed in the granulosa cells of the follicles is probably due to functional changes that occur during the process of cystogenesis.
  • [ X ]
    Öğe
    Effects of Potentilla fulgens as a Prophylactic Agent for Ischemia/Reperfusion Injury in the Rat Ovary
    (Sci Printers & Publ Inc, 2015) Togrul, Cihan; Balsak, Deniz; Ekinci, Cenap; Seckin, Kerem Doga; Ekinci, Aysun; Tahaoglu, Ali Emre; Bademkiran, Hanefi
    OBJECTIVE: To investigate the effects of Potentilla fulgens as a prophylactic agent on ischemia/reperfusion (I/R) injury in the rat ovary. STUDY DESIGN: A total of 32 Wistar rats were divided into 4 equal groups: (I) sham, (II) ischemia, (III) ischemia+reperfusion, and (IV) I/R+Potentilla fulgens. In groups I and II, ovary torsion was not performed and no drug was administered. In group III, 1 hour of ischemia and 2 hours of reperfusion were performed and no drug was given. Group IV received 400 mg/kg/day Potentilla fulgens intraperitoneally 5 days before I/R injury. RESULTS: The detorsion group showed preantral ovarian follicles and corpus luteum around the blood vessels and positive expression of vascular endothelial growth factor (VEGF). In the Potentilla fulgens group (IV) the stromal vascular endothelium with weak expression of VEGF was detected in small areas, and the ovarian follicles and the corpus luteum showed negative expression of VEGF. In the detorsion group the theca cells and apoptotic cells in preantral follicles showed positive expression of E-cadherin in the ovarian surface epithelium. Moreover, the E-cadherin expression was found to be positive in terms of follicular development, theca cells, granulosa cells, and corpus luteum. Potentilla fulgens, given after ischemic injury and apoptosis, was seen to decrease the effect of Bcl-2 expression. CONCLUSION: These results provide compelling evidence that the expression of E-cadherin in the ovary is an important component of ovarian function.