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Öğe Effect of extremely low frequency magnetic fields in safety standards on structure of acidophilic and basophilic cells in anterior pituitary gland of rats: An experimental study(2009) Akdag M.Z.; Dasdag S.; Ketani M.; Sagsoz H.After discovery of magnetite in the brain of several mammalian species including humans, it was suggested that the human brain is sensitive to magnetic fields. The present study investigated the effect of 100 ?T and 500 ?T extremely low frequency magnetic fields (ELF-MF), established guidelines by the International Commission on Non-Ionizing Radiation Protection (ICNIRP) for public and occupational magnetic field exposure limits at 50 Hz, on acidophilic and basophilic cells in the anterior pituitary gland of rats. 30 adult male Sprague Dawley rats were separated into three groups in this study (sham group: 10 and two study groups: 20). Rats in the study (experimental) groups separated equally into two groups that is 100 ?T and 500 ?T exposure groups. First experimental group rats exposed to 100 ?T while second experimental group exposed to 500 ?T ELF-MF for 2 hours per day (7 days in a week) during 10 months. For the sham group, the same experimental procedure was applied to the rats (2 hours/day/7 days in a week for 10 months) except ELF-MF exposure. After ELF-MF and sham exposure, the pituitary gland were removed totally to examine the structure of acidophlic and basophilic cells in the anterior pituitary gland of rats as histomorphometric and histochemical. Affinity against dye in acidophilic and basophilic cells of first experimental groups' rat was found lower than sham group. It means that degranulation may occur in sitoplasmic granuls of these cells. Disappearing in the affinity of Periodic Acid-Shchiff (PAS)-Orange-G in acidophilic and basophilic cells of second exposure group has been indicating high disappearing of granuls in these cells. No significant differences were found between sham and exposure groups in terms of the number of acidophilic and basophilic cells in the anterior pituitary gland (p>0.05). Although degranulation observed in acidophilic cells, the results were not found to be significant between experimental groups (p>0,05). However, degranulated basophilic cells were found significant between experimental groups (p<0,05). In conclusion, it was suggested that long-term ELF-MF exposure, which are the limits for public environmental and occupational magnetic field exposure guidelines, can affect affinity of PAS-Orange G in acidophilic and basophilic cells of the anterior pituitary gland in rats.Öğe Effects of propofol on expression ICAM-1 in rabbit gastric endothelial cells(2011) Ketani S.; Kanay B.E.; Sagsoz H.It was examined the dose-dependent effects of propofol on expression intracellular adhesion molecule (ICAM-1) in rabbit gastric endothelial cells. Twenty adult New Zeland albino rabbits were used in this study. One control and three experimantal groups designed. In experimantal groups 0.5, 4.0, 8.0 mg/kg propofol were applied to rabbits by marginal ear vein. One hour after applying propofol, control and experimantal group rabbits were sacrificed and their gaster were removed. The sections were stained with APAAP immunohistochemical staining for evaluation using a light microscope. No inflammatoric reactions were seen in sections of gastric endothelial cells of control and experimental groups.Öğe Immunohistochemical detection of estrogen and progesteron receptors in the bovine uterus and their relation to serum sex steroid hormone levels during the follicular and luteal phase(2009) Guney Saruhan B.; Sagsoz H.; Ketani M.A.; Akbalik M.E.; Ozyurtlu N.In this study, we tried to show the expression patterns of the steroid receptors in the bovine endometrium during the follicular and luteal phase. Samples of both uterus and blood were obtained from 30 adult, healty bovine at the moment of slaughter at a local slaughterhouse. Immunohistochemistry was performed by using rabbit polyclonal antibodies against the estrogen receptor (ER) and mouse monoclonal antibodies against the progesterone receptor (PR). In general, most of the uterine cells were stained positive but with a different intensity. During follicular phase, both ER and PR were obviously strong in the epithelia and the myometrium. For the glandular epithelium (GE), all GE cells were stained positive for ER and PR. When we compared both receptors during lutheal phase, a stronger intensity was observed in all compartments for PR, especially in the myometrium and surface-glandular epithelium. To summarize, the results from this study showed that both ER and PR might be regulated by the same mechanisms in some compartments and at specific stages of the oestrous cycle, and that each compartment of the uterus had a different expression of ER and PR which could accord with their different roles in reproductive physiology.Öğe Immunohistochemical localisation of oestrogen and epidermal growth factor receptors of the bitch uterus in the sexual cycle(2010) Ozyurtlu N.; Sagsoz H.; Saruhan B.G.; Zonturlu A.K.; Ketani M.A.; Akbalik M.E.The localisation of oestrogen (ER) and epidermal growth factor receptors (EGFR) in the various cell types of the bitch uterine was determined. In this study, 23 adult, healthy crossbred bitches brought to the clinic for ovariohisterectomy were used. ER and EGFR positive staining was detected in all cell types of the uterus. A distinct staining was seen in the luminal and glandular epithelium; while stromal and myometrial cells showed weak or moderate staining. The endothelial and smooth muscle cells of the vessels in the endometrium and myometrium sometimes appeared positive. No staining was observed in the mesothelium. The results of this study suggested that ER and EGFR were expressed at various levels in different cell types of bitch uterus. In light of the previous studies, and data of the presented investigations, it may be necesssary to elicit the harmonious proliferation and differentiation of epithelial and stromal cells that are considered essential for the preparation of the uterus for implantation.
