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    Blooming stages of Turkish hyssop (Hyssopus officinalis L.) affect essential oil composition
    (Taylor & Francis As, 2008) Kizil, Suleyman; Toncer, Ozlem; Ipek, Arif; Arslan, Neset; Saglam, Sevil; Khawar, Khalid Mahmood
    Hyssop (Hyssopus officinalis L.), native to the Caucasus, North Western Iran, Turkish North Eastern Black Sea region, and Southern Anatolia, is a highly valued medicinal plant. The experiment was conducted to find the effect of harvesting at different blooming stages of the plant on fresh and dry herbage yield, dry leaf yield, essential oil content, and essential oil components. In total, twenty-nine components were identified in hyssop essential oil by GC/MS. Isopinocamphone was the dominating component (47.9 51.4%) in the all analysed oil samples. The results clearly demonstrated that oil contents are seriously affected by the environmental conditions and stage of blooming, with the highest oil yield and oil contents at the post-blooming stage.
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    Öğe
    DIRECT BULBLET REGENERATION FROM STERNBERGIA FISCHERIANA (HERB.) RUPR. BULB SCALE EXPLANTS
    (Sejani Publ, 2014) Kizil, Suleyman; Khawar, Khalid Mahmood; Altuntas, Cigdem; Saglam, Sevil
    Attractive golden yellow flowered Sternbergia fischeriana (Herb.) Rupr. multiplies very slow under natural conditions. The study reports multiplication of plants using 0.5, 1.0, and 1.5 cm long bulb scale explants with two, three, four, and five scales attached by a thin base plate segment. Any concentration of 2,4-D 1.0, 2.0, 3.0, 4.0, and 5.0 mg l(-1) in MS medium was ineffective to induce bulblet regeneration on any explant at 15 degrees +/- 1 degrees C. Discursive induction of one or two 0.1 cm diameter bulblets was noted at 24 +/- 1 degrees C on 0.5 cm long two-scale explants. Variable regeneration was observed on 0.5, 1.0, and 1.5 cm long two-scale explants on MS basal medium containing combination of BAP and 0.2 mg l(-1) NAA. Maximum number of 5.0 +/- 0.5 bulblets per 0.5 cm long two-scale bulb explant was obtained on MS medium containing 8.5 mg l(-1) BAP plus 0.20 mg l(-1) NAA. The rooting as affected by the size of bulblet was achieved on MS medium containing 0.75 mg l(-1) NAA. The highest rooting was recorded on 0.47 cm diameter bulblets with 4.3 +/- 0.9 roots per bulblet and 3.7 +/- 0.4 cm long roots.