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    Geobacillus subterraneus subsp aromaticivorans subsp nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sirnak, Turkey
    (Microbiol Res Foundation, 2012) Poli, Annarita; Guven, Kemal; Romano, Ida; Pirinccioglu, Hamsi; Guven, Reyhan Gul; Euzeby, Jean Paul Marie; Matpan, Fatma
    A new thermophilic spore-forming strain Ge1(T) was isolated from the Guclukonak hot spring in Sirnak, Turkey. The strain was identified by using a polyphasic taxonomic approach. Strain Ge1(T) was Gram-positive, spore-forming, alkaliphilic rod-shaped, motile, occurring in pairs or filamentous. Growth was observed between 30 and 65 degrees C (optimum 60 degrees C) and at pH 5.5-10.0 (optimum pH 9.0). It was capable of utilizing starch, growth was observed at 0-3% NaCl (w/v) and was positive for catalase and urease. The major cellular fatty acids were iso-C-15:0 and iso-C-17:0, and the predominant lipoquinone found was menaquinone MK7 type. The DNA G+C content of the genomic DNA of strain Ge1(T) was 52.0%. Comparative 16S rRNA gene sequence studies showed that the isolate belonged to the genus Geobacillus. The DNA-DNA hybridization mean values between the representative strain Ge1(T) and the closely related species G. subterraneus, G. thermodenitrificans, G. thermocatenulatus, G. vulcani and G. thermoleovorans were 69.3%, 57%, 37%, 27% and 26%, respectively. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Ge1(T). Based on these results, we propose assigning a novel subspecies of Geobacillus subterraneus, to be named as Geobacillus subterraneus subsp. aromaticivorans subsp. nov. with the type strain Ge1(T) (DSM 23066 (T)= CIP 110341(T)).
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    Öğe
    A novel bio-solid phase extractor for preconcentrations of Hg and Sn in food samples
    (Academic Press INC Elsevier Science, 2022) Kılınç, Ersin; Özdemir, Sadin; Poli, Annarita; Niolaus, Barbara; Romano, Ida; Bekmezci, Muhammed; Şen, Fatih
    An ecofriendly preconcentration method was developed based on the use of Geobacillus galactosidasius sp. nov immobilized on Amberlite XAD-4 as an adsorbent for the preconcentrations of Hg and Sn. SEM-EDX performed for the investigation of surface functionality and morphology. The detailed investigations of factors such as pH of the solution, flow rate, interfering ions and sample volume have been thanks to the optimization of the preconcentration system. The optimum pHs were found as 5.0-7.0 for Hg and Sn and also the optimum flow rates were determined as 2 mL min(-1) for recovery of Hg and Sn. Under the best experimental conditions, limits of detections (LOD) were found as 0.53 ng mL(-1) for Hg and 0.27 ng mL(-1) for Sn. RSDs were calculated as 8.2% for Hg and 6.9% for Sn. The process was validated to use certified references (fish samples). ICP-OES was used to measure the levels of Hg and Sn in various real meal patterns after the devised technique was used. Concentrations of Hg and Sn were quantitively measured on gluten-free biscuit, flour, rice, Tuna fish, meat, chicken meat, potato, chocolate, coffee, tap water, energy drink and mineral water samples with low RSD. The developed method emerges as an innovative technology that will eliminate the low cost and toxic effect.