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Öğe Acinetobacter mesopotamicussp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey(Springer, 2020) Acer, Omer; Guven, Kemal; Poli, Annarita; Di Donato, Paola; Leone, Luigi; Buono, Lorena; Guven, Reyhan GulA new petroleum-degrading bacterium, designated strain GC2(T), was isolated from Bozkus 1 petroleum station in Diyarbakir, located in the southeast of Turkey. Cells were Gram-negative staining, aerobic, coccoid-rods, non-motile, non-spore-forming. The bacterium was found to degrade 100% ofn-alkanes ranging from C11 to C34 presented in the 1% crude oil after incubation of 7 days. The membrane phospholipids were 1,2 diacylglycero-3-phosphorylethanolamine (PEA), phosphatidylglycerol (PG), dipalmitoyl-sn-glycerol 1- phosphocholine (PC1), 1,2 dipalmitoyl-sn-glycero-3-phosphocholine monohydrate (PC3), cardiolipin also called diphosphatidylglycerol (CL) and l-alpha- phosphatidic acid, dipalmitoyl (AP); predominant respiratory ubiquinone was Q-8 and C16:0, C18:1 omega 9c and C16:1 were the major cellular fatty acids. The 16S rRNA sequence analysis revealed that the strain GC2(T)was a member of genusAcinetobacterand was most closely related toAcinetobacter lwoffiiDSM 2403( T)(99.79%),Acinetobacter pseudolwoffiiANC 5318( T)(98.83%) andAcinetobacter harbinensisHITLi 7( T)(98.14%). TherpoBandgyrBgene sequence analysis confirmed that the strain GC2(T)was a member of genusAcinetobacterand that the closest relative wasAcinetobacter lwoffiiDSM 2403( T)(99.08% and 100% similarity, respectively). DNA-DNA hybridization values between GC2(T)and its closest relatives ranged from 65.6% (withA. lwoffii) to 5.1% (withA. venetianus). The whole genome sequence of strain GC2(T)was obtained. The DNA G + C content of this strain was determined to be 42.9 mol %. ANI indexes, in silico estimations of DDH values and wet lab DDH values demonstrated that strain GC2(T)represents an independent genomospecies. On the basis of phenotypic characteristics, chemotaxonomic, phylogenetic data and DNA-DNA hybridization and whole genome analysis, we propose to assign strain GC2(T)as a new species of the genusAcinetobacter, for which the nameAcinetobacter mesopotamicussp. nov. is proposed. The type strain of this species is GC2(T)(DSM 26953 T = JCM 31073 T). The whole genome of strain GC2(T)has been deposited at DDBJ/ENA/GenBank under the accession JAALFF010000000.Öğe Anoxybacillus kamchatkensis subsp asaccharedens subsp nov., a thermophilic bacterium isolated from a hot spring in Batman(Microbiol Res Foundation, 2008) Gul-Guven, Reyhan; Guven, Kemal; Poli, Annarita; Nicolaus, BarbaraA new thermophilic spore-forming strain KG8(T) Was isolated from the mud of Taslidere hot spring in Batman. Strain KG8(T) was aerobe, Gram-positive, rod-shaped, motile, occurring in pairs or filamentous. Growth was observed from 35-65 degrees C (optimum 55 degrees C) and at pH 5.5-9.5 (optimum pH 7.5). It was capable of utilizing starch, growth was observed until 3% NaCl (w/v) and it was positive for nitrate reduction. On the basis of 16S rRNA gene sequence similarity, strain KG8(T) was shown to be related most closely to Anoxybacillus species. Chemotaxonomic data (major isoprenoid quinone-menaquinone-7; major fatty acid-iso-C15:0 and iso-C17:0) supported the affiliation of strain KG8(T) to the genus Anoxybacillus. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain KG8(T). Based on these results we propose assigning a novel subspecies of Anoxybacillus kamchatkensis, to be named Anoxybacillus kamchatkensis subsp. asaccharedens subsp. nov. with the type strain KG8(T) (DSM 18475(T)=CIP 109280(T)).Öğe Biosorption of Cd, Cu, Ni, Mn and Zn from aqueous solutions by thermophilic bacteria, Geobacillus toebii sub.sp decanicus and Geobacillus thermoleovorans sub.sp stromboliensis: Equilibrium, kinetic and thermodynamic studies(Elsevier Science Sa, 2009) Ozdemir, Sadin; Kilinc, Ersin; Poli, Annarita; Nicolaus, Barbara; Guven, KemalBiosorption of each of the ions Cd2+, Cu2+, Ni2+, Zn2+ and Mn2+ on Geobacillus toebii sub.sp. decanicus (G1) and Geobacillus thermoleovorans sub.sp. stromboliensis (G2) in a batch stirred system was investigated. The equilibrium adsorptive quantity was determined to be a function of the solution pH, contact time, biomass concentration, initial metal concentrations and temperature. The results obtained from biosorption experiments are used to understand the driving forces that govern the interaction between metal ions and a biosorbent. The experimental results were fitted well to the Scatchard plot, Langmuir, Freundlich, Dubinin-Radushkevich (D-R) isotherms. According to the parameters of the Langmuir isotherms, the maximum biosorption capacities of Cd2+, Cu2+, Ni2+, Zn2+ and Mn2+ for G2 were 38.8, 41.5, 42, 29 and 23.2 mg/g, respectively, while 29.2, 48.5, 21, 21.1 and 13.9 mg/g for G1, respectively. The mean free energy values evaluated from the D-R model indicated that the biosorptions of studied heavy metal ions onto bacteria were taken place by physical interaction. The biosorption mechanisms of studied metal ions on to the biosorbents were analysed by FT-IR spectra of G1 and G2 with and without metal ions. The first order and second order coefficients were obtained at 298, 308, 318 and 343 K. The experimental results were used to calculate the thermodynamic parameters. (C) 2009 Elsevier B.V. All rights reserved.Öğe Biosorption of Heavy Metals (Cd2+, Cu2+, Co2+, and Mn2+) by Thermophilic Bacteria, Geobacillus thermantarcticus and Anoxybacillus amylolyticus: Equilibrium and Kinetic Studies(Taylor & Francis Inc, 2013) Ozdemir, Sadin; Kilinc, Ersin; Poli, Annarita; Nicolaus, BarbaraTwo strains of thermophilic bacteria, Geobacillus thermantarcticus and Anoxybacillus amylolyticus, were employed to investigate the biosorption of heavy metals including Cd2+, Cu2+, Co2+, and Mn2+ ions. The effects of different biosorption parameters such as pH (2.010.0), initial metal concentrations (10.0300.0mg L-1), amount of biomass (0.2510g L-1), temperature (3080 degrees C), and contact time (15120min) were investigated. Concentrations of metal ions were determined by using an inductively coupled plasma optical emission spectrometry (ICP-OES). Optimum pHs for Cd2+, Cu2+, Co2+, and Mn2+ biosorption by Geobacillus thermantarcticus were found to be 4.0, 4.0, 5.0, and 6.0, respectively. For Anoxybacillus amylolyticus, the optimum pHs for Cd2+, Cu2+, Co2+, and Mn2+ biosorption were found to be 5.0, 4.0, 5.0, and 6.0, respectively. The Cd2+, Cu2+, Co2+, and Mn2+ removals at 50mg L-1 in 60min by 50mg dried cells of Geobacillus thermantarcticus were 85.4%, 46.3%, 43.6%, and 65.1%, respectively, whereas 74.1%, 39.8%, 35.1%, and 36.6%, respectively, for Anoxybacillus amylolyticus. The optimum temperatures for heavy metal biosorption were near the optimum growth temperatures for both strains. Scatchard plot analysis was employed to obtain more compact information about the interaction between metal ions and biosorbents. The plot results were further studied to determine if they fit Langmuir and Freundlich models.Öğe Cd, Cu, Ni, Mn and Zn resistance and bioaccumulation by thermophilic bacteria, Geobacillus toebii subsp decanicus and Geobacillus thermoleovorans subsp stromboliensis(Springer, 2012) Ozdemir, Sadin; Kilinc, Ersin; Poli, Annarita; Nicolaus, Barbara; Guven, KemalBioaccumulation and heavy metal resistance of Cd2+, Cu2+, Ni2+, Zn2+ and Mn2+ ions by thermophilic Geobacillus toebii subsp. decanicus and Geobacillus thermoleovorans subsp. stromboliensis were investigated. The metal resistance from the most resistant to the most sensitive was found as Mn > Ni > Cu > Zn > Cd for both Geobacillus thermoleovorans subsp. stromboliensis and Geobacillus toebii subsp. decanicus. It was determined that the highest metal bioaccumulation was performed by Geobacillus toebii subsp. decanicus for Zn (36,496 mu g/g dry weight cell), and the lowest metal bioaccumulation was performed by Geobacillus toebii subsp. decanicus for Ni (660.3 mu g/g dry weight cell). Moreover, the dead cells were found to biosorbe more metal in their membranes compared to the live cells. In the presence of 7.32 mg/l Cd concentration, the levels of Cd absorbed in live and dead cell membranes were found as 17.44 and 46.2 mg/g membrane, respectively.Öğe Geobacillus galactosidasius sp nov., a new thermophilic galactosidase-producing bacterium isolated from compost(Elsevier Gmbh, 2011) Poli, Annarita; Laezza, Giusi; Gul-Guven, Reyhan; Orlando, Pierangelo; Nicolaus, BarbaraTwo thermophilic spore-forming strains, with optimum growth temperature at 70 degrees C, were isolated from compost of the Experimental System of Composting (Teora, Avellino, Italy). A phylogenetic analysis based on 16S rRNA gene sequences showed that these organisms represented a new species of the genus Geobacillus. Based on polyphasic taxonomic data the strains represented a novel species for which the name Geobacillus galactosidasius sp. nov. is proposed. The type strain is CF1B(T) (=ATCC BAA-1450(T) = DSM 18751(T)). (C) 2011 Elsevier GmbH. All rights reserved.Öğe Geobacillus subterraneus subsp aromaticivorans subsp nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sirnak, Turkey(Microbiol Res Foundation, 2012) Poli, Annarita; Guven, Kemal; Romano, Ida; Pirinccioglu, Hamsi; Guven, Reyhan Gul; Euzeby, Jean Paul Marie; Matpan, FatmaA new thermophilic spore-forming strain Ge1(T) was isolated from the Guclukonak hot spring in Sirnak, Turkey. The strain was identified by using a polyphasic taxonomic approach. Strain Ge1(T) was Gram-positive, spore-forming, alkaliphilic rod-shaped, motile, occurring in pairs or filamentous. Growth was observed between 30 and 65 degrees C (optimum 60 degrees C) and at pH 5.5-10.0 (optimum pH 9.0). It was capable of utilizing starch, growth was observed at 0-3% NaCl (w/v) and was positive for catalase and urease. The major cellular fatty acids were iso-C-15:0 and iso-C-17:0, and the predominant lipoquinone found was menaquinone MK7 type. The DNA G+C content of the genomic DNA of strain Ge1(T) was 52.0%. Comparative 16S rRNA gene sequence studies showed that the isolate belonged to the genus Geobacillus. The DNA-DNA hybridization mean values between the representative strain Ge1(T) and the closely related species G. subterraneus, G. thermodenitrificans, G. thermocatenulatus, G. vulcani and G. thermoleovorans were 69.3%, 57%, 37%, 27% and 26%, respectively. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Ge1(T). Based on these results, we propose assigning a novel subspecies of Geobacillus subterraneus, to be named as Geobacillus subterraneus subsp. aromaticivorans subsp. nov. with the type strain Ge1(T) (DSM 23066 (T)= CIP 110341(T)).Öğe A novel bio-solid phase extractor for preconcentrations of Hg and Sn in food samples(Academic Press INC Elsevier Science, 2022) Kılınç, Ersin; Özdemir, Sadin; Poli, Annarita; Niolaus, Barbara; Romano, Ida; Bekmezci, Muhammed; Şen, FatihAn ecofriendly preconcentration method was developed based on the use of Geobacillus galactosidasius sp. nov immobilized on Amberlite XAD-4 as an adsorbent for the preconcentrations of Hg and Sn. SEM-EDX performed for the investigation of surface functionality and morphology. The detailed investigations of factors such as pH of the solution, flow rate, interfering ions and sample volume have been thanks to the optimization of the preconcentration system. The optimum pHs were found as 5.0-7.0 for Hg and Sn and also the optimum flow rates were determined as 2 mL min(-1) for recovery of Hg and Sn. Under the best experimental conditions, limits of detections (LOD) were found as 0.53 ng mL(-1) for Hg and 0.27 ng mL(-1) for Sn. RSDs were calculated as 8.2% for Hg and 6.9% for Sn. The process was validated to use certified references (fish samples). ICP-OES was used to measure the levels of Hg and Sn in various real meal patterns after the devised technique was used. Concentrations of Hg and Sn were quantitively measured on gluten-free biscuit, flour, rice, Tuna fish, meat, chicken meat, potato, chocolate, coffee, tap water, energy drink and mineral water samples with low RSD. The developed method emerges as an innovative technology that will eliminate the low cost and toxic effect.Öğe Purification and some properties of a ?-galactosidase from the thermoacidophilic Alicyclobacillus acidocaldarius subsp rittmannii isolated from Antarctica(Elsevier Science Inc, 2007) Gul-Guven, Reyhan; Guven, Kemal; Poli, Annarita; Nicolaus, BarbaraAn intracellular beta-galactosidase from a thermoacidophilic A licyclobacillus acidocaldarius subsp. rittmannii was purified by procedures including precipitation with ammonium sulphate, gel permeation, ion-exchange and affinity chromatography and finally by preparative electrophoresis and some properties of the purified enzyme were determined. The homogenous enzyme had a specific activity of 113 U/mg protein, with a fold purification of 163 and a yield of 8%. The Km and kcat values for ONPG were determined as 8.9 mM and 1074 min(-1), respectively in the purified beta-galactosidase from A. acidocaldarius subspecies rittmannii. The bacteria produce thermostable P-galactosidase (EC 3.2.1.23) activity, which exhibits its optimum at the neutral pH region. The pH and temperature optima for the purified enzyme are 6.0 and 65 degrees C, respectively (10 min assay). beta-Galactosidase specific activities of crude extracts obtained from bacterial cells grown in the presence and absence of lactose over a period of time (6-40 h) showed that beta-galactosidase synthesis seems to be constitutive and increases by increasing time up to 40 h of cultivation. beta-Galactosidase activity in the bacteria growing on the medium without lactose was 0.4 U/mg protein and increased up to 0.6 U/mg protein in the cells growing on the medium with lactose at 24 h (an increase by about 33% of its constitutive value), while it was 0.072 and 0.48 U/mg protein, respectively at 12 h (an increase by about 85% of its constitutive value). IPTG was also found to increase beta-galactosidase activity over a short period of time. The molecular mass of the purified enzyme determined by gel filtration on FPLC and SDS-PAGE was 165 and 76 kDa, respectively. The beta-galactosidase from Alicyclobacillus acidocaldarius subsp. rittmannii is most likely to belong to the glycoside hydrolyse family 42. (C) 2006 Elsevier Inc. All rights reserved.Öğe Resistance and bioaccumulation of Cd2+, Cu2+, Co2+ and Mn2+ by thermophilic bacteria, Geobacillus thermantarcticus and Anoxybacillus amylolyticus(Springer, 2013) Ozdemir, Sadin; Kilinc, Ersin; Nicolaus, Barbara; Poli, AnnaritaIn this study, bioaccumulation and heavy metal resistance of Cd2+, Cu2+, Co2+ and Mn2+ ions by thermophilic Geobacillus thermantarcticus and Anoxybacillus amylolyticus was investigated. The bacteria, in an order with respect to metal resistance from the most resistant to the most sensitive, was found to be Mn2+ > Co2+ > Cu2+ > Cd2+ for both G. thermantarcticus and A. amylolyticus. It was determined that the highest metal bioaccumulation was performed by A. amylolyticus in Mn2+ (28,566 mu g/g dry weight), and the lowest metal bioaccumulation was performed by A. amylolyticus in Co2+ (327.3 mu g/g dry weight). The highest Cd2+ capacities of dried cell membrane was found to be 36.07 and 39.55 mg/g membrane for G. thermantarticus and A. amylolyticus, respectively, and the highest Cd2+ capacities of wet cell membrane was found to be 14.36 and 12.39 mg/g membrane for G. thermantarcticus and A. amylolyticus, respectively.
