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Öğe The effect of progestagen on the changes of the vaginal flora arising from intravaginal sponge treatment and susceptibility of the vaginal flora to antibiotics in ewes(2008) Yesilmen S.; Ozyurtlu N.; Kucukaslan I.; Altan F.The aim of this study, was to investigate the effect of progestagen on the changes of the vaginal bacterial flora with sponge treatment. Progestagen impregnated sponges (30 mg fluorogestone acetate) were inserted ewes (Group I, n = 12) for 12 days and, sponge without progestagen (blank sponge), served as control groups (Group II, n = 12), were inserted ewes for 12 days during the non-breeding season. Vaginal bacterial counts were evaluated on the vaginal flora samples obtained before the introduction of the sponges, at sponge withdrawal and after 48 h from withdrawal of sponge. The mean value for the colony forming unite (x103 mL-1) were 6.1 and 4.5 on the day of intravaginal sponge insertion and increased to 113.5 and 139.8 at sponge withdrawal (p<0.05), decreased 7.9 and 43.3 after 48 h withdrawal of sponge in Group I and II, respectively (p<0.05). The changes of the vaginal bacterial flora were not different statistically at the time of sponge withdrawal in progestagen and non-progestagen sponge groups. Although, there were not differences between at the time of sponge introduction and withdrawal of sponge in 2 groups, it was found a difference after 48 h removal of sponges with progestagen and without progestagen treatments groups (p<0.05). Amoxicillin/Clavunate, Ampicillin, Oxacillin, Trimethoprim/Sulfamethoxazole 1/19 and Tetracycline were more resistance than the other antibiotics according to results of the antibiotic susceptibility test. Intravaginal sponge treatments increased bacterial counts, but this increase returned normal values at probable estrous time in progestagen impregnated sponge treatment. Number of vaginal bacteria did not return normal values in the non-progestagen sponge treatment group after 48 h removal of sponge, because of ewes in this group naturally could not come into estrus. In this study, it was concluded that progesterone did not affect the number of bacterial counts in the vaginal flora except for changes caused by intravaginal sponge treatment. © Medwell Journals, 2008.Öğe Effects of different intravaginal progesterone releasing devices on estrous synchronization and LH surge in fat-tailed ewes during non-breeding season(2007) Gungor O.; Cenesiz M.; Pancarci S.M.; Yildiz S.; Kaya M.; Kacar C.; Ozyurtlu N.The aims of this study were to compare two methods of estrus synchronization and to evaluate the effectiveness of the PMSG treatment combined with P4 application. Fifty non-lactating seasonal anestrus fat-tailed ewes were randomly assigned into five groups. The controlled internal drug release devices (CIDR) were applied during day 14 in group I and in group II. Progesterone impregnated sponges were applied during day 14 in group III and in group IV. And then 500 IU PMSG was injected in group I and III i.m. intravaginal devices removed. Ewes in group V served as controls. There was no difference between the groups in the peak value of LH and LH surge. Although LH surge was seen in the control group's 5 sheep, none of the control ewes expressed estrus. Different progestagen treatments have no different results when they are evaluated in terms of the success of the estrus synchronization. PMSG application, after P4 treatment, increased the success of the synchronization.Öğe Immunohistochemical detection of estrogen and progesteron receptors in the bovine uterus and their relation to serum sex steroid hormone levels during the follicular and luteal phase(2009) Guney Saruhan B.; Sagsoz H.; Ketani M.A.; Akbalik M.E.; Ozyurtlu N.In this study, we tried to show the expression patterns of the steroid receptors in the bovine endometrium during the follicular and luteal phase. Samples of both uterus and blood were obtained from 30 adult, healty bovine at the moment of slaughter at a local slaughterhouse. Immunohistochemistry was performed by using rabbit polyclonal antibodies against the estrogen receptor (ER) and mouse monoclonal antibodies against the progesterone receptor (PR). In general, most of the uterine cells were stained positive but with a different intensity. During follicular phase, both ER and PR were obviously strong in the epithelia and the myometrium. For the glandular epithelium (GE), all GE cells were stained positive for ER and PR. When we compared both receptors during lutheal phase, a stronger intensity was observed in all compartments for PR, especially in the myometrium and surface-glandular epithelium. To summarize, the results from this study showed that both ER and PR might be regulated by the same mechanisms in some compartments and at specific stages of the oestrous cycle, and that each compartment of the uterus had a different expression of ER and PR which could accord with their different roles in reproductive physiology.Öğe Immunohistochemical localisation of oestrogen and epidermal growth factor receptors of the bitch uterus in the sexual cycle(2010) Ozyurtlu N.; Sagsoz H.; Saruhan B.G.; Zonturlu A.K.; Ketani M.A.; Akbalik M.E.The localisation of oestrogen (ER) and epidermal growth factor receptors (EGFR) in the various cell types of the bitch uterine was determined. In this study, 23 adult, healthy crossbred bitches brought to the clinic for ovariohisterectomy were used. ER and EGFR positive staining was detected in all cell types of the uterus. A distinct staining was seen in the luminal and glandular epithelium; while stromal and myometrial cells showed weak or moderate staining. The endothelial and smooth muscle cells of the vessels in the endometrium and myometrium sometimes appeared positive. No staining was observed in the mesothelium. The results of this study suggested that ER and EGFR were expressed at various levels in different cell types of bitch uterus. In light of the previous studies, and data of the presented investigations, it may be necesssary to elicit the harmonious proliferation and differentiation of epithelial and stromal cells that are considered essential for the preparation of the uterus for implantation.Öğe [Seroprevalance of toxoplasmosis, leishmaiosis and listeriosis in shelter dogs of Diyarbakir, Turkey].(2010) Içen H.; Babür C.; Bademkiran S.; Celebi B.; Simşek A.; Ozyurtlu N.; Özkan A.T.The aim of this study was to determine the prevalence of toxoplasmosis, listeriosis and leishmaniasis in dogs in Diyarbakir region, Turkey. A total of 100 sera were collected from healthy dogs and tested for toxoplasmosis, leishmaniasis and listeriosis by the Sabin-Feldman Dye Test (SFDT), Indirekt Florescence Antikor Test (IFAT) and, Osebold Agglutination Test (OAT), respectively. Among these 100 dogs, 94 (94%) were seropositive for toxoplasmosis and and 17 (17%), for listeriosis. All of them were found to be seronegative for leishmaniasis. No statistically significant differences were observed between male and female dogs in the seroprevalence of toxoplasmosis and listeriosis. As a result, the presence of anti-Toxoplasma gondii and Listeria monocytogenesis specific antibodies in dogs in the region of Diyarbakir was determined.Öğe Synchronization of estrus using FGA and CIDR intravaginal pessaries during the transition period in Awassi ewes(2008) Zonturlu A.K.; Aral F.; Ozyurtlu N.; Yavuzer U.The objective of this study was to investigate the efficiency of synchronization using different progesterone treatments during transition period from the non-breeding to the natural breeding season. Thirty-four non-lactating Awassi ewes were randomly assigned to two groups, treated with fluorogestone acetate (FGA group, n = 19) or controlled internal drug release devices (CIDR group, n = 15) for 12 days. After pessary and sponges removal, all ewes received an intramuscular injection of 300 IU. eCG. There was no significant differences between treatments for ewes in estrus (FGA: 84.2%, CIDR: 86.6%). The onset of estrus from progestagen treatments, FGA and CIDR groups was (mean±SD.) 45.00±1.00 and 36.00±3.40, respectively. Interval from the cessation of treatment to the onset of estrus was significantly (p<0.05) longer in FGA, compared to the CIDR. The duration of the induced estrus period did not differ significantly between in 2 treatment groups. Pregnacy rate did not differ (p>0.05) between FGA (52.63%) and CIDR (60.00%). As a result, Estrus synchronization with FGA and CIDR is found to be similar, although the onset time of estrus varies with the use of FGA or CIDR. © Medwell Journals, 2008.
