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Öğe Anti-aging potential and anti-tyrosinase activity of three Hypericum species with focus on phytochemical composition by LC-MS/MS(Elsevier, 2019) Ersoy, Ezgi; Ozkan, Esra Eroglu; Boga, Mehmet; Yilmaz, Mustafa Abdullah; Mat, AfifeHypericum species are one of the most commonly used medicinal plants all over the world and traditional uses of the Hypericum species are mainly characterized by external applications for the treatment of dermatological disorders. The purpose of this study was to investigate the chemical compounds with anti-aging and anti-tyrosinase activities of Hypericum perforatum L., H. calycinum L., and H. confertum Choisy from Turkey and to bring innovation for further studies on developing formulations with Hypericum species for topical applications. In this study, chemical compositions of methanol extracts prepared from flowering aerial parts of the following species collected from Uludag, H. calycinum, H. confertum, and H. perforatum were investigated by LC-MS/MS. Nine metabolites, p-coumaric acid, gallic acid, quinic acid, chlorogenic acid, malic acid, protocatechuic acid, rutin, quercitrin, and isoquercitrin were identified in all analyzed species. To investigate the anti-aging and skin-whitening properties, enzyme inhibition activity of the three species were tested against, collagenase, elastase, hyaluronidase, and tyrosinase enzymes. H. calycinum appeared the most active in all enzyme inhibition activity assays which is mainly due to its rich phenolic content. The methanol extract of H. calycinum showed the highest collagenase, elastase and hyaluronidase inhibitory activities with IC50 values of 51.24 +/- 1.33 mu g/mL, 55.77 +/- 1.32 mu g/mL and 22.17 +/- 0.02 mu g/mL, respectively. Additionally, H. calycinum emerged as the most potential tyrosinase inhibitor among these species with 54.30 +/- 0.49% at 200 mu g/mL concentration. The most abundant compounds determined in H. calycinum methanol extract are, chlorogenic acid (2595.15 +/- 0.179 mu g/g), quercitrin (1805.9 +/- 0.98 mu g/g), quinic acid (885.50 +/- 0.073 mu g/g) and isoquercitrin (346.16 +/- 0.046 mu g/g), respectively. Our study demonstrates that all three extracts, especially H. calycinum extract are potential agents to use in cosmeceuticals for anti-aging and skin-whitening purposes.Öğe Evaluation of in vitro biological activities of three Hypericum species ( H. calycinum, H. confertum , and H. perforatum ) from Turkey(Elsevier, 2020) Ersoy, Ezgi; Ozkan, Esra Eroglu; Boga, Mehmet; Mat, Afife[Abstract Not Available]Öğe Identification and quantification of phenolic and volatile constituents in five different Anatolian thyme species using LC-MS/MS and GC-MS, with biological activities(Elsevier, 2021) Boga, Mehmet; Ozkan, Esra Eroglu; Ersoy, Ezgi; Tuncay, Eyyup; Canturk, Yeter Yesil; Cinar, Ercan; Kara, Emel MataraciBeing widely used in the food industry, thyme is one of the most valuable plants in many aspects, and Turkey is one of the hotspots for thyme industry. Five thyme species, Thymus convolutus (endemic), Thymus fallax, Thymus haussknechtii Velen (endemic), Thymus kotschyanus var. kotschyanus, and Thymus sipyleus from Turkey were subjected in this study. Their essential oils and the extracts of aerial and root parts were obtained. GC-MS analyses showed that camphor was the major compound in T. convolutus (12.7%), and in T. sipyleus (13.1%) essential oils. The major volatile compound of T. fallax was bicyclogermacrene (21.5%), whereas the T. kotschyanus var. kotschyanus essential oil showed a predominance of carvacrol (48.5%), and thymol (22.5%). Carvacrol was also the major compound of T. haussknechtii with 28.2%. LC-MS/MS analyses showed that nicotiflorin (293.28-4201.15 mu g/mL), caffeic (516.41-851.28 mu g/mL), quinic (344.37-4410.94 mu g/mL), rosmarinic (1800.18-9631.71 mu g/mL), and fumaric acid (350.88-682.10 mu g/mL) were detected as the main compounds in the extracts. All extracts exerted strong antioxidant activity, and the highest radical scavenging activity was demonstrated by T. haussknechtii root extract. T. convolutus and T. haussknechtii aerial parts extracts showed significant antimicrobial activity against C. tropicalis at MIC of 19.53 mu g/mL. The extracts were found to be nontoxic against COLO205 and KM12 human colon, and UO-31, and A498 human renal cell lines. Their enzyme inhibitory activities were also evaluated against cholinesterase, tyrosinase and urease enzymes and T. fallax aerial part extract was the most effective with 34.48 +/- 0.60% in antibutyrylcholinesterase activity. These species with strong antioxidant and antifungal properties and no toxicity could be beneficial to be used as preservatives in the food processing industry.Öğe LC-HRMS Profiling of Phytochemicals with Assessment of Antioxidant, Anticholinesterase, and Antimicrobial Potentials of Astragalus Brachystachys DC(Wiley-V C H Verlag Gmbh, 2024) Ersoy, Ezgi; Boga, Mehmet; Kaplan, Alevcan; Kara, Emel Mataraci; Ozkan, Esra Eroglu; Kayiran, Serpil DemirciAstragalus species are ubiquitous in the pharmacopeia of numerous countries, signifying their widespread medicinal applications. T & uuml;rkiye is esteemed as one of the principal epicenters of diversity for this genus with 483 taxa, and many of these plants have been traditionally utilized for diseases including coughing, diabetes, cardiovascular disorders, and aches. Although there is a considerable body of chemical and biological research available on several Astragalus species, studies focusing on Astragalus brachystachys DC are exceedingly rare. In this context, This study provides the first comprehensive report on this medicinal plant the chemical constituents and biological activities of an important medicinal plant, Astragalus brachystachys DC. The aerial part samples were collected from Adana, T & uuml;rkiye, and an ethanol extract was prepared with these parts. The secondary metabolites of the extract were determined by an LC-HRMS analysis. The LC-HRMS analysis showed the presence of 39 different constituents, hyperoside (303.419 +/- 10.50 mu g/g extract), p-coumaric acid (256.975 +/- 8.51 mu g/g extract), and rutin (72.684 +/- 2.23 mu g/g extract) were determined as major compounds in the aerial parts ethanol extract. Attributed to its high total phenolic (58.53 +/- 1.30 mu g PEs/mg extract) and total flavonoid content (29.98 +/- 0.83 mu g QEs/mg extract), the extract demonstrated strong antioxidant activity according to three different assays namely DPPH free (IC50: 33.08 +/- 0.61 mu g/mL), and ABTS cation radical scavenging (IC50: 15.39 +/- 0.72 mu g/mL) and CUPRAC activity (A0.5: 36.25 +/- 0.28 mu g/mL) methods. In vitro assays showed that cholinesterase inhibitory activity results were found to be exceptional with 85.95 +/- 0.52 % inhibition on acetylcholinesterase and 66.32 +/- 1.33 % inhibition on butyrylcholinesterase at 200 mu g/mL. Regarding antimicrobial properties, Astragalus brachystachys DC extract was found to be effective against Enterococcus faecalis with a MIC value of 39.06 mu g/mL.Öğe LC-MS/MS analyses of Ziziphora clinopodioides Lam. from Turkey: Antioxidant, anticholinesterase, antimicrobial and, anticancer activities(Istanbul Univ, Fac Pharmacy, 2020) Ozkan, Esra Eroglu; Boga, Mehmet; Yilmaz, Mustafa Abdullah; Kara, Emel Mataraci; Yesil, YeterBackground and Aims: Ziziphora clinopodioides is one of the wild edible species used in Turkish folk medicine. The study was aimed to investigate the phenolic compounds of the extracts as well as the antioxidant, enzyme inhibitory, antimicrobial activities, and anticancer potential. Methods: LC-MS/MS was used to determine phenolic compounds; antioxidant potential was evaluated by using radical scavenging assays; enzyme inhibition assays were used for anticholinesterase, tyrosinase, and urease activities; antimicrobial activities were performed by microdilution method; and XTT bioassay was used for testing anticancer potential. Results: The LC-MS/MS results indicate that quinic acid (9020.51 +/- 73.97 mu g/g; 14721.04 +/- 120.71 mu g/g, respectively) is the major compound, malic acid (1972.95 +/- 22.29 mu g/g; 2179.04 +/- 24.62 mu g/g, respectively) and rhoifolin (1044.74 +/- 98.31 mu g/g; 3593.31 +/- 338.13 mu g/g, respectively) are the abundant compounds in aerial and root extracts. The antioxidant activity results showed that the aerial parts extract has stronger ABTS cation radical and DPPH free radical scavenging activity than the root extract with 40.90 +/- 0.19 mu g/mL, and 94.27 +/- 0.64 mu g/mL IC50 values, respectively. The extracts showed moderate cupric reducing activity with 1.74 absorbance value at 100 mu g/mL. Only the aerial parts extract exhibited weak tyrosinase inhibition (8.60 +/- 0.87%) compared with kojic acid (95.26 +/- 0.23%) at 200 mu g/mL. No activity was observed in urease and anticholinesterase enzymes. Both extracts exhibited moderate antifungal activity against Candida tropicalis with 39.06 mu g/mL MIC values. The extracts didn't show cytotoxic activity against Renal (A498, UO-31) and Colo (COLO205, KM12) cell lines, also there is no metastatic potential of the extracts against osteosarcoma cell lines (MG63.3, MG63). Conclusion: Z. clinopodioides has rich phytochemical constituents with powerful health benefits, and doesn't have any harmful effect on the body.
