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    CD34 expression of chorionic villous in pre-eclamptic placenta: an immunohistochemical and ultrastructural study
    (I R O G Canada, Inc, 2013) Kalkanli, S.; Deveci, E.; Sak, M. E.; Evsen, M. S.; Baran, O.; Ozekinci, S.; Yavuz, D.
    In this study, pre-eclampsia, proteinuria, and edema associated with hypertension in pregnancy were assessed at the Dicle University School of Medicine Department of Obstetrics and Gynecology Clinic. One group included 20 pre-eclamptic pregnant women with gestational age 20-35 weeks of pregnancy and the same in the control group that included; however, 20 normotensive pregnant women. Histochemistry, immunohistochemistry, and electron microscopy techniques were used. Histopathological examination of syncytial nodes and stromal cells were observed in the increase in hyperplasia and hyalinization. The evaluation immnunohistochemical of chorionic villi, placenta, and hematopoietic stem cell markers showed a positive reaction with CD34. Ultrastructural examination showed endoplasmic reticulum dilatation, degeneration of mitochondria in endothelial cells, and capillary vessel edema.
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    Contribution of BAP1 loss and p16 (CDKN2A) deletion analysis to the definitive diagnosis of mesothelioma in effusion cytology
    (Verduci Publisher, 2023) Aydogdu, G.; Ozekinci, S.
    OBJECTIVE: The cytological diagnosis of mesothelioma is a controversial is-sue, and definitive diagnosis often requires ancillary tests. The aim of this study was to investigate the contribution of BRCA1-associated protein (1) (BAP1) loss and p16 (CDKN2A) homozygous deletion (HD) on the early diagnosis of mesothelioma in effusion fluids. MATERIALS AND METHODS: Between 2019-2022, 21 pleural and peritoneal fluid samples di-agnosed with atypical mesothelial proliferation in our institution were included in the study. The slides of the cases that underwent BAP1 immu-nohistochemistry (IHC) were retrieved from the archive and re-examined. Homozygous deletion (HD) of p16 (CDKN2A) was investigated by the fluorescence in situ hybridization (FISH) method in cell blocks of cytology samples. At least 100 atypical mesothelial cells were counted in each case, and the HD threshold value was >10%. RESULTS: The mean age of the cases was 63.47 years (34-90 years), female/male ratio was 3/1. Of the pleural mesothelioma cases, 16 were epithelioid, 2 were biphasic, and 1 were sarco-matoid. Two cases were diagnosed with perito-neal well-differentiated papillary mesothelioma (WDPM). BAP1 loss was observed in 11 (69%) of 16 cases. HD deletion of p16 (CDKN2A) was seen in 11 (58%) patients with FISH. The HD threshold value was 10-20% in 6 of the cases, 30-50% in 3 cases, and above 90% in 2 cases. While HD de-letion was observed in p16 (CDKN2A) in all bi-phasic and sarcomatoid cases (n=3), no deletion was observed in peritoneal WDPM (n=2). Positiv-ity was observed with at least one method in 12 (86%) of 14 pleural mesotheliomas who under-went both BAP1 IHC and p16 (CDKN2A) FISH. Due to technical reasons, the FISH signal could not be obtained in two cell blocks, so no results could be obtained. CONCLUSIONS: Asbestos exposure in ar-eas where mesothelioma is endemic and/or the presence of proliferating mesothelial cells in cy-tological examination are important clues for diagnosis. In controversial cases, BAP1 IHC should be the first step in an ancillary test. Al-though the FISH method applied to cell blocks has cytology-specific limitations and difficul-ties, investigating the p16 (CDKN2A) deletion with FISH in selected cases will contribute to the diagnosis.
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    EXPRESSION OF E-CADHERIN, COX-2, P53 AND BCL-2 IN PROSTATE CARCINOMAS: CORRELATION WITH TUMOR DIFFERENTIATION AND METASTATIC POTENTIAL
    (Taylor & Francis Ltd, 2010) Ozekinci, S.; Uzunlar, A. K.; Senturk, S.; Gedik, Abdullah; Buyukbayram, H.; Mizrak, B.
    Prognostic factors of prostate cancer such as PSA stage Gleason grade and surgical margins have limitations they are inable to indicate metastatic potential The aim of this study was to investigate expression of e-cadherin cox-2 p53 and bcl-2 in prostate carcinomas and to search association with tumor differentiation and metastatic potential Eighty-seven prostate cancer specimens were retreived from tissue blocks embedded in parafin and expression patterns of e-cadherin cox-2 p53 and bcl-2 were investigated by immunoperoxidase method Gleason scoring system was used for tumor grading and expression patterns of these antibodies were compared among different tumor grades Seven cases (8%) had well-differentiated 39(45%) had moderately differentiated and 41 (47%) had poorly differentiated tumors Metastases were present in 31 patients (35%) There was a statistically significant association between tumor differentiation and expression of e-cadherin or bcl-2 (p < 0 01) however no association was found between tumor differentiation and expression of cox-2 or p53 (p > 0 01) There was no association between expressions of e-cadherin cox-2 p53 or bcl-2 and metastasis E-cadherin expression is less frequent in poorly differentiated tumors whereas bcl-2 expression is more frequent However there is no relation with metastatic potential and expression of these markers Expression of cox-2 and p53 is neither related to the degree of differentiation nor to the metastatic potential