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Öğe Accumulation and histopathological effects of cadmium on the great pond snail Lymnaea stagnalis Linnaeus, 1758 (Gastropoda: Pulmonata)(Elsevier, 2020) Karakas, Serpil Burcun; Otludil, BirgulToxic metal ions are an important stress factor for a living organism. In this study, accumulation and histopathological changes in foot, mantle and hepatopancreas of great pond snail, Lymnaea stagnalis exposed to different Cadmium (Cd) concentrations in laboratory conditions were investigated. Great pond snails were exposed to sublethal concentrations of 7.92 mu g/L, 15.85 mu g/L, 31.7 mu g/L and 63.4 mu g/L Cd. At the end of 7, 14, 21 and 28 days, snail foot, mantle and hepatopancreas were removed to investigate and determine Cd accumulation and histopathological alterations by light microscopy. Cd levels determined in hepatopancreas were higher than those measured from the foot and the mantle of studied specimens. A positive correlation was found between Cd levels in tissues and exposure days. As a result of Cd application, we observed increase in the number of mucosit, pigment and protein cells and desquamation in the epithelium in the foot, atrophy in muscle fibrils, connective tissue cells and increase in the lipid vacuoles in the mantle, increase in the lipid vacuoles and amoebocyte in the hepatopancreas. The severity of the alterations resulting from Cd increased with dose-time dependent.Öğe Effect of Copper Sulphate (CuSO4) on Freshwater Snail, Physa acuta Draparnaud, 1805: A Histopathological Evaluation(Springer, 2020) Otludil, Birgul; Ayaz, SabahatIn this study, freshwater snail (Physa acuta) was investigated to determine histopathological effects of CuSO4 on digestive gland, foot, mantle and ovotestis under laboratory conditions. The snails were exposed to different sublethal concentrations of CuSO4 (0.05 mg/L, 0.1 mg/L and 0.2 mg/L) periods of 10, 20 and 30 days. The relationship between CuSO4 concentration and mortality rate in snails was calculated as Y = 8.8 + 125.14X, R-2 = 0.9444. The histopathological examinations revealed that CuSO4 caused significant histopathological changes in all the tissues of the snail. The severity of these lesions in tissues increased with increasing CuSO4 concentration and duration of exposure. The results showed that freshwater snail, Physa acuta can be considered to be a suitable bioindicator to demonstrate the toxic effect of copper in aquatic environments.Öğe An examination on composition of spermatozoa obtained from pre-operative and post-operative varicocele patients(Inst Animal Reproduction Food Research, 2018) Afsin, Muhamet; Otludil, Birgul; Dede, Onur; Akkus, MuratVaricocele is one of the main and surgically repairable causes of male infertility, which arises from dilatation and torsion of the testicular veins in the plexus pampiniformis. In this study, we examined semen samples from 40 patients diagnosed with varicocele between the ages of 15 and 30 years, according to WHO criteria (pre-operatively, and at 3, 6, and 12 months post-operatively). The mean spermatozoa concentration was found to be 45.25 +/- 34.83 million/ml pre-operatively, while the mean post-operative concentration was 48.85 +/- 35.73 million/ml at three months, 51.72 +/- 32.82 million/ml at six months, and 49.63 +/- 28.05 million/ml at 12 months (P > 0.05). The mean rate of A + B motile spermatozoa was 35.5 +/- 14.71% pre-operatively, 42.65 +/- 16.80% at three months, 43 +/- 13.52%at six months and 44 +/- 14.76 percent at 12 months post-operatively (P < 0.05). The mean Kruger morphology score was 3.15 +/- 3.0% pre-operatively, and 3.20 +/- 2.83% at three months, 2.97 +/- 2.61%at six months and 3.27 +/- 2.50%at 12 months post-operatively (P > 0.05). The nucleus, acrosomal cap, mitochondrial structure and microtubules of the tail of the spermatozoa were examined under electron microscopy. The mean DNA fragmentation index (DFI%) of the spermatozoa was 20.57 +/- 4.60% pre-operatively, and post-operatively at 17.27 +/- 3.65% at three months, 15.5 +/- 3.23% at six months and 15.3 +/- 3.63% at 12 months (P < 0.001). The findings suggest that despite the increased count and motility, as well as the improved DNA fractures observed post-operatively in the spermatozoa of varicocele patients, the morphology rates remain intact.Öğe Healing effect on cadmium toxicity of EDTA in Lymnaea stagnalis(Elsevier Sci Ltd, 2011) Karakas, Serpil Burcun; Otludil, Birgul[Abstract Not Available]
